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Biomolecules stations
Biomolecules stations

... 7. Answer question #9 on your sheet. 8. Now begin to fold your protein according to the chemical properties of the sidechains. Remember all of these chemical properties affect the protein at the same time. a. Fold your protein so that all of the hydrophobic sidechains are buried on the inside of you ...
Course details
Course details

... • 2-color was originally designed due to problems in making reproducible arrays - e.g. the ratio on a spot is more reproducible than the absolute intensity if the spot size/concentration changes from array-to-array. • With 2-colors, you don’t necessarily get twice as much data since it is typically ...
Foundations of Biology
Foundations of Biology

... of a group of genes (i.e., heat shock proteins) A single gene may be regulated by a number of independent transcription factors (i.e., metallothionein) Eukaryotic regulation does not seem to involve repression To achieve high levels of expression, several different transcription factors binding to d ...
PIR-International Protein Sequence Database
PIR-International Protein Sequence Database

... of annotation, a minimal level of redundancy and a high level of integration with other databases. ...
Identification of amino acid sequence by X
Identification of amino acid sequence by X

... example of such a case is the elucidation from a crystallographic structure of the post-translational chromophore (Fig. 2) formation in the green fluorescent protein, GFP (Ormo et al., 1996; Yang et al., 1996; Palm et al., 1997). It is also possible to deduce the nature of chemical modifications by ...
Research
Research

... in nearly every biology and biochemistry textbook. At the time, this discovery was thought to explain all of biology, health, and disease. However, only focusing on the expression of a single gene or protein neglects the multi-faceted consequences of that expression on metabolism, as well as overall ...
Core promoter
Core promoter

... were required for the timed regulation of developmental events.  Since then, hundreds of microRNAs have been identified in almost all metazoan genomes, including worms, flies, plants and mammals.  MicroRNAs have diverse expression patterns and might regulate various developmental and physiological ...
Intragenic Suppression of a Capsid Assembly-Defective
Intragenic Suppression of a Capsid Assembly-Defective

... Phage/plasmid crosses: Crosses to move gene 9 alleles between P22 phage and plasmid replicons were performed as described in SCHWARZ and BERGET(1989a). pJS28 is a pBR322-derived plasmidwhich carries the tailspike gene under the control of the lacUV5 promoter. It also contains the origin of replicati ...
A Survey of Recent Work on Evolutionary Approaches to the Protein
A Survey of Recent Work on Evolutionary Approaches to the Protein

... high density of side-chains in the protein interior, sidechain conformations are determined by packing considerations, which involve interactions of many side-chains at a time. But homology modeling is not the only reason this problem is of interest. An ecient solution to this problem is essential ...
A dietary supplement is intended to provide nutrients that may
A dietary supplement is intended to provide nutrients that may

... and recovery times. In addition, recent studies have also shown that creatine improves brain function and reduces mental fatigue.Unlike steroids or other performance-enhancing drugs, creatine can be found naturally in many common foods such as herring, tuna, salmon, and beef. Creatine increases what ...
Quorum sensing by peptide pheromones and two-component
Quorum sensing by peptide pheromones and two-component

... of the bioluminescence genes. In many other Gram-negative species, similar autoinducing circuits have been identified that utilize similar components (AHL, LuxI and LuxR homologues) and play a role in cell-density-dependent regulation of cellular physiology (for a review see Salmond et al ., 1995). ...
Novel In Vitro Method for Screening Inhibitors of Protein Translation
Novel In Vitro Method for Screening Inhibitors of Protein Translation

... expression in these experiments could potentially serve as internal control making sure the IVT expression system is functional. In Panel C, instead of having two separate mRNA’s as in Panel B, a single dual-luciferase mRNA containing both Tluc and RFF on the same construct was expressed. Again, m7G ...
2.4 Proteins
2.4 Proteins

... Essential Idea • Proteins have a very wide range of functions in living organisms. ...
Protein Modeling Challenge Science Olympiad Trial Event
Protein Modeling Challenge Science Olympiad Trial Event

... RasMol is a great program and may be continued to be used by Science Olympiad Teams. However, since there have not been updates to RasMol to allow for it to operate easily on Macs, Jmol will be the computer visualization program to be used in the future. The commands that you have used in RasMol are ...
CHMI 2227E Biochemistry I
CHMI 2227E Biochemistry I

... Example of protein purification Apoptosis: a form of cell death Current Opinion in Cell Biology 2004, 16:663–669 ...
secstruct_PT
secstruct_PT

... example, in drug design) and biotechnology (for example, in the design of novel enzymes) ...
Ammonium transport in Escherichia coli: localization and nucleotide
Ammonium transport in Escherichia coli: localization and nucleotide

... subclone. The Amt-complementing insert of pJH 1A (Jayakumar et ul., 1989) was subcloned into the pBluescript KS(+) vector as a 3-4 kb HindIIILBamHI fragment. The resulting plasmid (pKSAMT) was digested with Sac1 and BamHI, or KpnI and HindIII. Each linearized plasmid was then subjected to exonucleas ...
Reproductive Tract Infections
Reproductive Tract Infections

... 160kDa protein we encountered difficulties in identifying the cDNA due to very weak reaction. In addition, sequence of these positive clones were found to be associated with partial sequence of host genome. This may be due to inadvertent recombination betweet the cDNA sequence of the protein and the ...
The Building Blocks Teacher Key
The Building Blocks Teacher Key

... Rice and beans combine to make a complementary protein. If toast and peanuts were combined, they would also make a complementary protein. Rice and beans are complementary proteins because they are each missing different amino acids. When combined, the missing amino acids are found in the other to ma ...
i PRODUCTION OF NATURAL PROTEIN USING CHICKEN
i PRODUCTION OF NATURAL PROTEIN USING CHICKEN

... dissolves in ethanol and methanol, though it exhibits lower solubility in these solvents than does potassium hydroxide. Molten sodium hydroxide is also a strong base, but the high temperature required limits applications. It is insoluble in ether and other non-polar solvents. A sodium hydroxide solu ...
Aphelenchoides besseyi
Aphelenchoides besseyi

... many other crops. Fatty acid and retinoid binding protein (FAR) is a specific protein in nematodes and is related to development, reproduction, infection to the host, and disruption of plant defense reactions, so the inhibition of FAR function is the potential approach to control A. besseyi. The ful ...
ADP-ribosyltransferases: plastic tools for inactivating protein and
ADP-ribosyltransferases: plastic tools for inactivating protein and

... Some ADPRTs exhibit constitutive activity, others require proteolytic cleavage, reduction of a disulphide bond, or association with other proteins for activation. For example, diphtheria toxin is made as an inactive multidomain precursor protein (Wilson and Collier, 1992). The receptor-binding domai ...
2005 MCB 3020 Study Objectives, Part 2
2005 MCB 3020 Study Objectives, Part 2

... prokaryotic intrinsic terminators (but not rho-dependent terminators). Know that the Pribnow box is another name for the prokaryotic –10 consensus sequence. • Be sure you can distinguish between components/events that occur in transcription versus translation. For ex., RNA polymerase binds to the pr ...
book ppt - Castle High School
book ppt - Castle High School

... In the disease β-thalassemia, a mutation may occur at an intron consensus sequence in the β-globin gene—the premRNA can not be spliced correctly. Non-functional β-globin mRNA is produced, which shows how mutations are used to elucidate cause-and-effect relationships. Alternative splicing results in ...
Chapter 10 DNA to Protein
Chapter 10 DNA to Protein

... In the disease β-thalassemia, a mutation may occur at an intron consensus sequence in the β-globin gene—the premRNA can not be spliced correctly. Non-functional β-globin mRNA is produced, which shows how mutations are used to elucidate cause-and-effect relationships. Alternative splicing results in ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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