LabM3bioinformatics
... As the proteins with similar functions contain homologus amino acid sequences that corresponds to important functional domains in the three dimensional structure of the proteins, so the function of a protein that is not been isolated often can be predicted based on the homology of its gene or cDNA w ...
... As the proteins with similar functions contain homologus amino acid sequences that corresponds to important functional domains in the three dimensional structure of the proteins, so the function of a protein that is not been isolated often can be predicted based on the homology of its gene or cDNA w ...
Gene Section LOXL4 (lysyl oxidase-like 4) Atlas of Genetics and Cytogenetics
... Note: LOXL4 (lysyl oxidase-like 4) is a member of the lysyl oxidase (LOX) family. Its C-terminal region is conserved in all five members of this copper-dependent amine oxidase family and includes a copper-binding site, lysyl and tyrosine residues that form the lysyltyrosine-quinone cofactor (LTQ) an ...
... Note: LOXL4 (lysyl oxidase-like 4) is a member of the lysyl oxidase (LOX) family. Its C-terminal region is conserved in all five members of this copper-dependent amine oxidase family and includes a copper-binding site, lysyl and tyrosine residues that form the lysyltyrosine-quinone cofactor (LTQ) an ...
Exam 2
... a. DNA-dependent DNA polymerase. b. DNA-dependent RNA polymerase. c. RNA-dependent DNA polymerase. d. RNA-dependent RNA polymerase. e. reverse transcriptase. ...
... a. DNA-dependent DNA polymerase. b. DNA-dependent RNA polymerase. c. RNA-dependent DNA polymerase. d. RNA-dependent RNA polymerase. e. reverse transcriptase. ...
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... 17. Humans first applied genetics to the domestication of plants (wheat, peas, etc.) and animals (dogs, goats, etc.) between approximately 10,000 and 12,000 years ago. (T) ...
... 17. Humans first applied genetics to the domestication of plants (wheat, peas, etc.) and animals (dogs, goats, etc.) between approximately 10,000 and 12,000 years ago. (T) ...
Template for PowerPoint Use
... Often species-specific and cell-type specific, with no clinical symptoms ...
... Often species-specific and cell-type specific, with no clinical symptoms ...
DNA notes
... •This is still a wide open area of research, but it is becoming clear that there are several ways to do this. Most of the molecular work has been done using plasmids (they are partition into daughter cells too) and we will look at that closely when we discuss plasmids later. •For now look at the sys ...
... •This is still a wide open area of research, but it is becoming clear that there are several ways to do this. Most of the molecular work has been done using plasmids (they are partition into daughter cells too) and we will look at that closely when we discuss plasmids later. •For now look at the sys ...
Finding Genes in Eukaryotes
... of introns and exons, and the analysis must detect the intron/exon boundaries and assemble the exons into a contiguous coding sequence. There are two basic approaches to detecting which ORFs are actually coding regions, i.e., genes. These approaches either rely on detecting SIGNALS or detecting CONT ...
... of introns and exons, and the analysis must detect the intron/exon boundaries and assemble the exons into a contiguous coding sequence. There are two basic approaches to detecting which ORFs are actually coding regions, i.e., genes. These approaches either rely on detecting SIGNALS or detecting CONT ...
Ch. 13.4: DNA Applications
... 1. Why does gene expression need to be regulated? (Are all genes expressed present in a cell expressed? Why or why not?) 2. How does gene regulation in prokaryotes differ from regulation in eukaryotes? a. Prokaryotic Gene Expression Describe the control mechanism of the Lac operon (or operon syste ...
... 1. Why does gene expression need to be regulated? (Are all genes expressed present in a cell expressed? Why or why not?) 2. How does gene regulation in prokaryotes differ from regulation in eukaryotes? a. Prokaryotic Gene Expression Describe the control mechanism of the Lac operon (or operon syste ...
3/27
... • Goal: to measure RNA levels of all genes in genome • RNA levels vary with the following: – Cell type – Developmental stage – External stimuli ...
... • Goal: to measure RNA levels of all genes in genome • RNA levels vary with the following: – Cell type – Developmental stage – External stimuli ...
Finding Genes in Eukaryotes
... Usually the primary challenge that follows the sequencing of anything from a small segment of DNA to a complete genome is to establish where the various functional elements such as genes, promoters, terminators etc., lie in the sequence. This module concentrates on the identification of regions of D ...
... Usually the primary challenge that follows the sequencing of anything from a small segment of DNA to a complete genome is to establish where the various functional elements such as genes, promoters, terminators etc., lie in the sequence. This module concentrates on the identification of regions of D ...
Gene Section DDX43 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 43) Atlas of Genetics and Cytogenetics
... 55% similarity with the human p68 protein, an ATPdependent RNA helicase that is a member of the DEAD-box proteins. Four motifs that are present in members of the DEAD box family are conserved in the HAGE protein. However, ATPase and helicase activities of HAGE were not demonstrated. ...
... 55% similarity with the human p68 protein, an ATPdependent RNA helicase that is a member of the DEAD-box proteins. Four motifs that are present in members of the DEAD box family are conserved in the HAGE protein. However, ATPase and helicase activities of HAGE were not demonstrated. ...
Biology Topic 3
... Originally developed by bacteria for defense against viruses, restriction enzymes cut DNA only at specific sequences, allowing two different DNA strands to be cut with the same restriction enzyme and reattached. DNA fragments from another organism are then cleaved by the same restriction enzyme as ...
... Originally developed by bacteria for defense against viruses, restriction enzymes cut DNA only at specific sequences, allowing two different DNA strands to be cut with the same restriction enzyme and reattached. DNA fragments from another organism are then cleaved by the same restriction enzyme as ...
Genetic Engineering
... Transformation The components of the mixture of recombinant and other plasmid molecules formed by ligation must now be isolated from one another and replicated (cloned) by transfer into a host organism. By far the most common hosts for simple cloning experiments are strains of E. coli which have spe ...
... Transformation The components of the mixture of recombinant and other plasmid molecules formed by ligation must now be isolated from one another and replicated (cloned) by transfer into a host organism. By far the most common hosts for simple cloning experiments are strains of E. coli which have spe ...
Viruses Lecture 1
... Viruses are most numerous “microorganisms” on the planet. Infect ALL types of cellular organisms All viruses are the same (strategically)! Viruses package their genome into a particle which is then infectious The viral genome has all of the information that it needs to attach and enter the host, dec ...
... Viruses are most numerous “microorganisms” on the planet. Infect ALL types of cellular organisms All viruses are the same (strategically)! Viruses package their genome into a particle which is then infectious The viral genome has all of the information that it needs to attach and enter the host, dec ...
RNA Phage
... - do not code for any proteins - do not require helper virus - infected tissue contains no virus-like particles - replicated by host cell machinery (but RNA!!!) When compare strains and natural recombinants: - find conserved functional domains ...
... - do not code for any proteins - do not require helper virus - infected tissue contains no virus-like particles - replicated by host cell machinery (but RNA!!!) When compare strains and natural recombinants: - find conserved functional domains ...
Chapter 6 Notes
... • Diploid number (2n): body cells (all cells excluding sperm and egg) have two sets of chromosomes. The diploid number for humans is 46 (2 x 23). Humans inherit one set of 23 from their male parent and one set of 23 from their female parent. • Haploid number (n): gametes (sperm and egg cells) have o ...
... • Diploid number (2n): body cells (all cells excluding sperm and egg) have two sets of chromosomes. The diploid number for humans is 46 (2 x 23). Humans inherit one set of 23 from their male parent and one set of 23 from their female parent. • Haploid number (n): gametes (sperm and egg cells) have o ...
What is trans-acting factor?
... repression that can spread along chromatin, switching off multiple genes without the need for each to bear binding sites for specific repressor. Insulator elements can block this spreading, so insulators protect genes from both indiscriminate activation and repression。 ...
... repression that can spread along chromatin, switching off multiple genes without the need for each to bear binding sites for specific repressor. Insulator elements can block this spreading, so insulators protect genes from both indiscriminate activation and repression。 ...
PDF
... potential. By analysing REST-depleted ES cells, the researchers demonstrate that neural determinants, such as MASH1 and neurogenin 1 and 2, remain appropriately repressed, and that the multi-lineage potential and dominant reprogramming capacity of ES cells do not depend on REST. However, a group of ...
... potential. By analysing REST-depleted ES cells, the researchers demonstrate that neural determinants, such as MASH1 and neurogenin 1 and 2, remain appropriately repressed, and that the multi-lineage potential and dominant reprogramming capacity of ES cells do not depend on REST. However, a group of ...
Bioproduction of recombinant protein
... Here, we present the results of our bioproduction models. Firstly, we determine the best conditions for CHO-S cell transduction (Rate of transduced cells and fluorescent intensity level) using concentrated GFP lentiviral suspension. Secondly, protein of interest is produced using cDNA-His tagged-exp ...
... Here, we present the results of our bioproduction models. Firstly, we determine the best conditions for CHO-S cell transduction (Rate of transduced cells and fluorescent intensity level) using concentrated GFP lentiviral suspension. Secondly, protein of interest is produced using cDNA-His tagged-exp ...
Lambda Vectors and their replication
... • Phage can alternate between lysogenic (non-productive) and lytic (productive) growth cycles. ...
... • Phage can alternate between lysogenic (non-productive) and lytic (productive) growth cycles. ...
II. Changes in chromosome number
... 1. Homozygosity for a deletion is often, but not always, lethal 2. Heterozygosity for a deletion is often detrimental 3. Heterozygosity for deletions affects mapping distances 4. Deletions in heterozygotes can “uncover” genes 5. Using deletions to locate genes B. Duplications add material to the gen ...
... 1. Homozygosity for a deletion is often, but not always, lethal 2. Heterozygosity for a deletion is often detrimental 3. Heterozygosity for deletions affects mapping distances 4. Deletions in heterozygotes can “uncover” genes 5. Using deletions to locate genes B. Duplications add material to the gen ...
Gene and Chromosome
... childhood, however some, such as Huntington's disease, can escape detection until the patient is well into adulthood. The basic aspects of a genetic disorder rests on the inheritance of genetic material. With an in depth family history, it is possible to anticipate possible disorders in children whi ...
... childhood, however some, such as Huntington's disease, can escape detection until the patient is well into adulthood. The basic aspects of a genetic disorder rests on the inheritance of genetic material. With an in depth family history, it is possible to anticipate possible disorders in children whi ...
bioinformatics
... The study of the origin & descent of spp.and their change over time. New insight to molecular basis of disease. Investigating the function of homologs of a disease gene. Homology:two genes sharing a common evolut.history. Finding evolut.relationships between diff.forms of life. Closely related orgni ...
... The study of the origin & descent of spp.and their change over time. New insight to molecular basis of disease. Investigating the function of homologs of a disease gene. Homology:two genes sharing a common evolut.history. Finding evolut.relationships between diff.forms of life. Closely related orgni ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.