Biotechnology - Department of Plant Biology
... To use cloning to produce multiple copies of a gene, it is first necessary to identify and isolate an initial sample of that gene. There are several strategies to do this; the choice depends on the information available. One set of methods starts with a purified protein, whereas another set starts w ...
... To use cloning to produce multiple copies of a gene, it is first necessary to identify and isolate an initial sample of that gene. There are several strategies to do this; the choice depends on the information available. One set of methods starts with a purified protein, whereas another set starts w ...
Supplementary Information (doc 68K)
... and then incubated for 16 hours at 37 0C and 5% CO2. Subsequently, cells were washed three times in PBS / 2% FCS and stained with an anti-HLA-A*0201-FITC antibody (BB7.2, BD Biosciences) to determine increase in HLA expression by flow cytometry on a FACS Calibur (BD Biosciences). Influenza peptide ( ...
... and then incubated for 16 hours at 37 0C and 5% CO2. Subsequently, cells were washed three times in PBS / 2% FCS and stained with an anti-HLA-A*0201-FITC antibody (BB7.2, BD Biosciences) to determine increase in HLA expression by flow cytometry on a FACS Calibur (BD Biosciences). Influenza peptide ( ...
Anti-TXLNB antibody ab133202 Product datasheet 2 Images Overview
... Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We invest ...
... Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We invest ...
Leukaemia Section del(11)(q23q23) MLL/ARHGEF12 Atlas of Genetics and Cytogenetics in Oncology and Haematology
... domain: methyltransferase; methylates H3, including histones in the HOX area for allowing chromatin to be open to transcription. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus: a p300/320 Nterm protein called MLL-N, and a p180 C-term protein, called MLL-C. The FYRN and a FRY ...
... domain: methyltransferase; methylates H3, including histones in the HOX area for allowing chromatin to be open to transcription. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus: a p300/320 Nterm protein called MLL-N, and a p180 C-term protein, called MLL-C. The FYRN and a FRY ...
wk10-ManjHIV
... OVERVIEW OF HIV-1 infection 3. Integration - viral DNA joins host DNA 4. Transcription- making multiple viral RNAs 5.Translation – producing viral proteins. 6. Viral Protease- cleaving viral proteins. When viral RNA is translated into protein, that protein is assembled in a long chain that includes ...
... OVERVIEW OF HIV-1 infection 3. Integration - viral DNA joins host DNA 4. Transcription- making multiple viral RNAs 5.Translation – producing viral proteins. 6. Viral Protease- cleaving viral proteins. When viral RNA is translated into protein, that protein is assembled in a long chain that includes ...
SECTION D What Does DNA Do?
... protein might change drastically. A gene, which is a functional unit of DNA, carries coded information indicating the precise sequence in which amino acids should be strung together to make one particular kind of protein that will play one particular role in the life of the cell. ...
... protein might change drastically. A gene, which is a functional unit of DNA, carries coded information indicating the precise sequence in which amino acids should be strung together to make one particular kind of protein that will play one particular role in the life of the cell. ...
File
... 1. In transcription, a section of DNA unwinds and nucleotides on DNA form base pairs with nucleotides of messenger RNA, creating an strand of mRNA. 2. This segment of mRNA then leaves the cell nucleus, headed for a ribosome in the cell’s cytoplasm, where ...
... 1. In transcription, a section of DNA unwinds and nucleotides on DNA form base pairs with nucleotides of messenger RNA, creating an strand of mRNA. 2. This segment of mRNA then leaves the cell nucleus, headed for a ribosome in the cell’s cytoplasm, where ...
SMU-DDE-Assignments-Scheme of Evaluation PROGRAM msc
... • When the cells are starved for glucose, the cell produces a pool of cAMP molecules. These cAMP molecules bind to proteins called Catabolite activator proteins (CAP) also called as cAMP receptor proteins or CRPs. • This CAP cAMP complex then binds to a site near the lac operon’s promoter called CAP ...
... • When the cells are starved for glucose, the cell produces a pool of cAMP molecules. These cAMP molecules bind to proteins called Catabolite activator proteins (CAP) also called as cAMP receptor proteins or CRPs. • This CAP cAMP complex then binds to a site near the lac operon’s promoter called CAP ...
Foundations of Biology
... Some of the general methods used to control expression in prokaryotes are used in eukaryotes, but nothing resembling operons is known Eukaryotic genes are controlled individually and each gene has specific control sequences preceding the transcription start site In addition to controlling transcript ...
... Some of the general methods used to control expression in prokaryotes are used in eukaryotes, but nothing resembling operons is known Eukaryotic genes are controlled individually and each gene has specific control sequences preceding the transcription start site In addition to controlling transcript ...
Repressilator
... So, what is it? • An oscillatory network • A genetic construction with three genes, each one regulates the next • Repressor depending regulation negative feedback • “a 3-element negative feedback transcriptional loop” • “tide producing machine” ...
... So, what is it? • An oscillatory network • A genetic construction with three genes, each one regulates the next • Repressor depending regulation negative feedback • “a 3-element negative feedback transcriptional loop” • “tide producing machine” ...
Transcription Initiation
... Some of the general methods used to control expression in prokaryotes are used in eukaryotes, but nothing resembling operons is known Eukaryotic genes are controlled individually and each gene has specific control sequences preceding the transcription start site In addition to controlling transcript ...
... Some of the general methods used to control expression in prokaryotes are used in eukaryotes, but nothing resembling operons is known Eukaryotic genes are controlled individually and each gene has specific control sequences preceding the transcription start site In addition to controlling transcript ...
Transcription and Translation
... (RER) – makes proteins that leave the cell (insulin, hormones, enzymes) ...
... (RER) – makes proteins that leave the cell (insulin, hormones, enzymes) ...
Describe the structure/ function of blood cell types
... Hematopoiesis involves cytokine signaling ...
... Hematopoiesis involves cytokine signaling ...
The Cell, 5e
... • Stromal cells secrete growth factors • Cytokines signal via membrane receptors ...
... • Stromal cells secrete growth factors • Cytokines signal via membrane receptors ...
Influence of insertion site of the avian influenza virus haemagglutinin
... analysis. Total RNA was isolated from the virus-infected CEFs, separated in denaturing agarose gels, transferred to nylon membranes and hybridized with 32P-labelled antisense RNA specific for AIV H5, AIV N1 or NDV P. The signals were quantified by radioluminography using a FLA3000 scanner (Fujifilm) ...
... analysis. Total RNA was isolated from the virus-infected CEFs, separated in denaturing agarose gels, transferred to nylon membranes and hybridized with 32P-labelled antisense RNA specific for AIV H5, AIV N1 or NDV P. The signals were quantified by radioluminography using a FLA3000 scanner (Fujifilm) ...
Genetic_Research_Lesson9_Slides_Single_Sequence_NWABR
... Circle #1: Example of a series of the same nucleotide (many T’s in a row). Notice the highest peaks are visible at each position. Circle #2: Example of an ambiguous base call. Notice the T (Red) at position 57 (highlighted in blue) is just below a green peak (A) at the same position. Look at the poo ...
... Circle #1: Example of a series of the same nucleotide (many T’s in a row). Notice the highest peaks are visible at each position. Circle #2: Example of an ambiguous base call. Notice the T (Red) at position 57 (highlighted in blue) is just below a green peak (A) at the same position. Look at the poo ...
A phage library and two cosmid libraries were
... The six V R regions located on the phage and cosmid clones of Fig. 1 were sequenced and compared to each other and to the sequence of the mouse V_ probe which was used to isolate the phages Ch1 and Ch2 (Fig. 2 ) . The sequence of VI turned out to be identical to the sequence of HK102 which had been ...
... The six V R regions located on the phage and cosmid clones of Fig. 1 were sequenced and compared to each other and to the sequence of the mouse V_ probe which was used to isolate the phages Ch1 and Ch2 (Fig. 2 ) . The sequence of VI turned out to be identical to the sequence of HK102 which had been ...
Transcription – Gene regulation
... - the motif might not be well conserved between promoters - the sequences used to search for the motif do not necessarily represent the complete promoter - genes with promoters to be analyzed are in many cases grouped together by a clustering algorithm which has its own limitations. ...
... - the motif might not be well conserved between promoters - the sequences used to search for the motif do not necessarily represent the complete promoter - genes with promoters to be analyzed are in many cases grouped together by a clustering algorithm which has its own limitations. ...
GO: The Gene Ontology
... Additional points • A gene product can have several functions, cellular locations and be involved in many processes • Annotation of a gene product to one ontology is independent from its annotation to other ontologies • Annotations are only to terms reflecting a normal activity or location • Usage ...
... Additional points • A gene product can have several functions, cellular locations and be involved in many processes • Annotation of a gene product to one ontology is independent from its annotation to other ontologies • Annotations are only to terms reflecting a normal activity or location • Usage ...
Flow of genetic information DNA --> RNA -
... Unfinished HTG sequences containing contigs greater than 2 kb are assigned an accession number and deposited in the HTG division. A typical HTG record might consist of all the first pass sequence data generated from a single cosmid, BAC, YAC, or P1 clone which together comprise more than 2 kb and co ...
... Unfinished HTG sequences containing contigs greater than 2 kb are assigned an accession number and deposited in the HTG division. A typical HTG record might consist of all the first pass sequence data generated from a single cosmid, BAC, YAC, or P1 clone which together comprise more than 2 kb and co ...
Tools for transcription factor research
... vectors, for in vivo analysis. Our in vitro assays for transcription factor (TF) profiling include our protein/DNA (PD) arrays that can be used to monitor TF expression levels when cells are perturbed by various stimuli or as they are subjected to a change in their physiological state. This approa ...
... vectors, for in vivo analysis. Our in vitro assays for transcription factor (TF) profiling include our protein/DNA (PD) arrays that can be used to monitor TF expression levels when cells are perturbed by various stimuli or as they are subjected to a change in their physiological state. This approa ...
D - mbg
... UNIQUELY AMONG CONDENSING ENZYMES, 3-‐HYDROXY-‐3-‐METHYLGLUTARYL (HMG)–COA SYNTHASE (HMGS) CATALYZES THE FORMATION OF A CARBON–CARBON BOND BY ACTIVATING THE METHYL GROUP OF AN ACETYLATED CYSTEINE. THIS REACTION IS ...
... UNIQUELY AMONG CONDENSING ENZYMES, 3-‐HYDROXY-‐3-‐METHYLGLUTARYL (HMG)–COA SYNTHASE (HMGS) CATALYZES THE FORMATION OF A CARBON–CARBON BOND BY ACTIVATING THE METHYL GROUP OF AN ACETYLATED CYSTEINE. THIS REACTION IS ...
X - Genaxxon bioscience
... Synonyms: CDF, HILDA, D-Factor, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. ...
... Synonyms: CDF, HILDA, D-Factor, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. ...
View PDF - CiteSeerX
... MCGGAII/L and cannot be assigned to any of the three previously de¢ned ERF classes [8]. This N-terminal motif is only found in ERF genes, including AtEBP from Arabidopsis [23] and OsEBP-89 from rice [20]. LeERF2, therefore, de¢nes the new ERF class IV. Though the function of the MCGGAII/L motif has ...
... MCGGAII/L and cannot be assigned to any of the three previously de¢ned ERF classes [8]. This N-terminal motif is only found in ERF genes, including AtEBP from Arabidopsis [23] and OsEBP-89 from rice [20]. LeERF2, therefore, de¢nes the new ERF class IV. Though the function of the MCGGAII/L motif has ...
haemoglobin abnormalities
... tested. Antenatal diagnosis is offered if both are affected as there is a risk of a severe fetal Hb defect, particularly β-thalasaemia major. Fetal DNA analysis can be carried out using amniotic fluid, chorionic villus or fetal blood samples. Abortion is offered if the fetus is found to be affecte ...
... tested. Antenatal diagnosis is offered if both are affected as there is a risk of a severe fetal Hb defect, particularly β-thalasaemia major. Fetal DNA analysis can be carried out using amniotic fluid, chorionic villus or fetal blood samples. Abortion is offered if the fetus is found to be affecte ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.