GeneChip Hybridization
... Probe sets: The DNA oligo probe is attached to the GeneChip via a silane bond ...
... Probe sets: The DNA oligo probe is attached to the GeneChip via a silane bond ...
pGLO Transformation Lab Introduction to Transformation In this lab
... transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, you will learn about the process of moving genes from one organism to another with the a ...
... transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, you will learn about the process of moving genes from one organism to another with the a ...
DNA Lesson Plan - Penn Arts and Sciences
... other nucleotides to form the phosphate sugar backbone: figure 5b and 5c. Figure 5c and figure 6 show the 5’ and 3’ ends of the helix, stress this orientation for RNA transcription. Figure 6 ends the review of DNA structure as it positions all of the structures into the helix. Students should see th ...
... other nucleotides to form the phosphate sugar backbone: figure 5b and 5c. Figure 5c and figure 6 show the 5’ and 3’ ends of the helix, stress this orientation for RNA transcription. Figure 6 ends the review of DNA structure as it positions all of the structures into the helix. Students should see th ...
A question of taste
... been found. As a comparison, the mouse genome contains 33 bitter taste genes but only three pseudogenes. The presence of multiple taste receptor genes that are obviously related shows that several gene duplication events have taken place. Different animals live in very different environments and the ...
... been found. As a comparison, the mouse genome contains 33 bitter taste genes but only three pseudogenes. The presence of multiple taste receptor genes that are obviously related shows that several gene duplication events have taken place. Different animals live in very different environments and the ...
Identification of Virgibacillus species using 16S rRNA gene Sequence
... A comparison of the 16s rRNA gene sequence of the test strain with the non -redundant collection (Genbank, DDBJ, EMBL & PDB) of sequences was performed using BLAST . A number of sequences of the genus Virgibacillus aligned with 16S rRNA gene sequence of test strain. We then developed a multiple sequ ...
... A comparison of the 16s rRNA gene sequence of the test strain with the non -redundant collection (Genbank, DDBJ, EMBL & PDB) of sequences was performed using BLAST . A number of sequences of the genus Virgibacillus aligned with 16S rRNA gene sequence of test strain. We then developed a multiple sequ ...
Review-examII-2010
... ___________ strand is synthesized continuously while the (c) _________ strand is synthesized discontinuously. On the strand synthesized discontinuously, the short pieces are called (d) ____________ fragments. An RNA primer for each of the fragments is synthesized by an enzyme called (e) __________, ...
... ___________ strand is synthesized continuously while the (c) _________ strand is synthesized discontinuously. On the strand synthesized discontinuously, the short pieces are called (d) ____________ fragments. An RNA primer for each of the fragments is synthesized by an enzyme called (e) __________, ...
Attenuation regulation of amino acid biosynthetic operons in
... 3.1. Isoleucine, leucine, and valine biosynthesis Orthologs of the branched-chain amino acids (ILV) genes in genomes of c-, b- and a-proteobacteria were identified by similarity search. Positional gene clusters corresponding to possible ILV operons are shown in Table 2. Then, the LLLM program was app ...
... 3.1. Isoleucine, leucine, and valine biosynthesis Orthologs of the branched-chain amino acids (ILV) genes in genomes of c-, b- and a-proteobacteria were identified by similarity search. Positional gene clusters corresponding to possible ILV operons are shown in Table 2. Then, the LLLM program was app ...
Laboratory 11
... and environmental samples. Some methods are very complicated while others are quite simple. Your choice of technique depends on the specific sample that you are working with and your requirements for the quality of DNA extracted. All of the methods include: a step for breaking open (lysing) the cell ...
... and environmental samples. Some methods are very complicated while others are quite simple. Your choice of technique depends on the specific sample that you are working with and your requirements for the quality of DNA extracted. All of the methods include: a step for breaking open (lysing) the cell ...
Job listing details - University of Florida
... long-term career goals; date of availability to begin postdoc research/technician 2) Curriculum vitae (including publications and awards/honors) 3) List of at least three references Dr. Mingyi Xie was trained in the laboratory of Dr. Joan Steitz at Yale University (2010-2016), where he documented tw ...
... long-term career goals; date of availability to begin postdoc research/technician 2) Curriculum vitae (including publications and awards/honors) 3) List of at least three references Dr. Mingyi Xie was trained in the laboratory of Dr. Joan Steitz at Yale University (2010-2016), where he documented tw ...
The evolutionary history of the stearoyl
... duplicated members in expected regions of the NKparalogon. The HNRNPD/HNRPDL pair has a paralogue mapping to Hsa5 at 4 Mb from the NK-like homeobox gene MSX2. However, the most striking case is represented by the SEC31 gene family, which is composed of two members: SEC31A and SEC31B. SEC31A maps nex ...
... duplicated members in expected regions of the NKparalogon. The HNRNPD/HNRPDL pair has a paralogue mapping to Hsa5 at 4 Mb from the NK-like homeobox gene MSX2. However, the most striking case is represented by the SEC31 gene family, which is composed of two members: SEC31A and SEC31B. SEC31A maps nex ...
Electra Vector System™
... almost any cloning or expression vector to function in the Electra System. Easy – 1 tube, 5 minute reaction Universal – any ORF cloned into any Electra vector. Quickly move ORFs from MOTHER vector to multiple DAUGHTER vectors Scarless – Always in frame with no nucleotide scars IP-Free – No l ...
... almost any cloning or expression vector to function in the Electra System. Easy – 1 tube, 5 minute reaction Universal – any ORF cloned into any Electra vector. Quickly move ORFs from MOTHER vector to multiple DAUGHTER vectors Scarless – Always in frame with no nucleotide scars IP-Free – No l ...
mRNA
... as a template upon which a complementary strand of RNA is assembled from nucleotides • In contrast with DNA replication, only part of one DNA strand, not the whole molecule, is used as a template for transcription © Cengage Learning 2015 ...
... as a template upon which a complementary strand of RNA is assembled from nucleotides • In contrast with DNA replication, only part of one DNA strand, not the whole molecule, is used as a template for transcription © Cengage Learning 2015 ...
PCR-based gene synthesis to produce recombinant proteins for
... Scheme 2of error correction using coupled methods of site directed mutagenesis and homologous recombination Scheme of error correction using coupled methods of site directed mutagenesis and homologous recombination. Assembled synthetic gene fragments subcloned into a plasmid vector are subjected ini ...
... Scheme 2of error correction using coupled methods of site directed mutagenesis and homologous recombination Scheme of error correction using coupled methods of site directed mutagenesis and homologous recombination. Assembled synthetic gene fragments subcloned into a plasmid vector are subjected ini ...
MGI-Jan2007 - Gene Ontology Consortium
... Alex also prepared a major revision to the Cell Ontology covering hematopoietic cell types in concert with the work on the GO immunology terms. This revision included 80 new cell types, as well as the provision of definitions for many existing cell types, is_a links to root for all hemopoietic cell ...
... Alex also prepared a major revision to the Cell Ontology covering hematopoietic cell types in concert with the work on the GO immunology terms. This revision included 80 new cell types, as well as the provision of definitions for many existing cell types, is_a links to root for all hemopoietic cell ...
Biotechnology toolkit part 2
... Exons are the coding parts of a gene and introns are the non-coding parts of a gene which are removed before translation (splicing). About 90% of the human genome has no known function and mainly consists on intron. Exons that code for the amino acid sequence in essential proteins vary little, sinc ...
... Exons are the coding parts of a gene and introns are the non-coding parts of a gene which are removed before translation (splicing). About 90% of the human genome has no known function and mainly consists on intron. Exons that code for the amino acid sequence in essential proteins vary little, sinc ...
Automated Targeted Locus Amplification for Targeted
... rapid targeted complete sequencing of the BRCA1 gene from the human Coriell 12878 cell-line. The entire workflow and automation protocol for the generation of TLA templates and subsequent target locus amplification is identical for the amplification of any gene(s) of interest, only a different set o ...
... rapid targeted complete sequencing of the BRCA1 gene from the human Coriell 12878 cell-line. The entire workflow and automation protocol for the generation of TLA templates and subsequent target locus amplification is identical for the amplification of any gene(s) of interest, only a different set o ...
Automated Targeted Locus Amplification (TLA) Technology for
... rapid targeted complete sequencing of the BRCA1 gene from the human Coriell 12878 cell-line. The entire workflow and automation protocol for the generation of TLA templates and subsequent target locus amplification is identical for the amplification of any gene(s) of interest, only a different set o ...
... rapid targeted complete sequencing of the BRCA1 gene from the human Coriell 12878 cell-line. The entire workflow and automation protocol for the generation of TLA templates and subsequent target locus amplification is identical for the amplification of any gene(s) of interest, only a different set o ...
ORS 2017 Annual Meeting Poster No.1864
... synovial joints, including cartilage fibrillation, synovial reaction, osteophyte formation, and subchondral bone changes [1]. The present study aimed to investigate whether NFAT1 deficiency affects cellular function, tissue metabolism, and development of intervertebral disc degeneration (IDD) in mic ...
... synovial joints, including cartilage fibrillation, synovial reaction, osteophyte formation, and subchondral bone changes [1]. The present study aimed to investigate whether NFAT1 deficiency affects cellular function, tissue metabolism, and development of intervertebral disc degeneration (IDD) in mic ...
Protein Synthesis
... • Takes places in the nucleus of the cell The process by which the information from DNA is transferred to RNA. DNA uncoils and unzips. • The exposed DNA bases are matched up with RNA bases in the nucleus to form mRNA. ...
... • Takes places in the nucleus of the cell The process by which the information from DNA is transferred to RNA. DNA uncoils and unzips. • The exposed DNA bases are matched up with RNA bases in the nucleus to form mRNA. ...
Preparation of MyoD mRNA for the differentiation of stem cells into
... In the lab, the mRNA will be transcribed using in vitro transcription of the MyoD DNA template. In vitro transcription can use several different RNA polymerases, such as T7 and SP6. In order for the RNA polymerases to transcribe the DNA, a polymerase specific promoter region has to be present on the ...
... In the lab, the mRNA will be transcribed using in vitro transcription of the MyoD DNA template. In vitro transcription can use several different RNA polymerases, such as T7 and SP6. In order for the RNA polymerases to transcribe the DNA, a polymerase specific promoter region has to be present on the ...
Cloning Vectors A cloning vector is a DNA molecule that can carry
... transfected cells can be identified by plating on a medium containing the antibiotic. Those cells which did not take up the cosmid would be unable to grow. Unlike plasmids, they can also be packaged in phage capsids, which allow the foreign genes to be transferred into or between cells by transducti ...
... transfected cells can be identified by plating on a medium containing the antibiotic. Those cells which did not take up the cosmid would be unable to grow. Unlike plasmids, they can also be packaged in phage capsids, which allow the foreign genes to be transferred into or between cells by transducti ...
Can pseudocomplementary peptide nucleic acid nucleases
... advantages when used in vivo (Figure.2B). Because the backbone of PNAs is neutral, complementary PNA/DNA binds tighter than a comparable DNA/DNA duplex due to the lack of charge repulsion between the two sequences. Single base mismatches in PNA/DNA duplex are more destabilized than equivalent one in ...
... advantages when used in vivo (Figure.2B). Because the backbone of PNAs is neutral, complementary PNA/DNA binds tighter than a comparable DNA/DNA duplex due to the lack of charge repulsion between the two sequences. Single base mismatches in PNA/DNA duplex are more destabilized than equivalent one in ...
Lecture 2
... R.Franklin, English biophysicist and X-ray crystallographer, took X-ray diffraction photomicrographs of crystalline DNA extract, the key to the puzzle. Franklin’s colleague Maurice Wilkins shared her data, without her knowledge, with J.Watson and F.Crick. It was Watson and Crick who put all the piec ...
... R.Franklin, English biophysicist and X-ray crystallographer, took X-ray diffraction photomicrographs of crystalline DNA extract, the key to the puzzle. Franklin’s colleague Maurice Wilkins shared her data, without her knowledge, with J.Watson and F.Crick. It was Watson and Crick who put all the piec ...
DNA amplification 2
... original starter DNA sample. Similarly, absence of a band may indicate that the target sequence was not present in the original starter DNA sample. In this way, PCR can be used in combination with other techniques to not just simply amplify DNA (which, in essence, is all it does!) but also to detect ...
... original starter DNA sample. Similarly, absence of a band may indicate that the target sequence was not present in the original starter DNA sample. In this way, PCR can be used in combination with other techniques to not just simply amplify DNA (which, in essence, is all it does!) but also to detect ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.