Lecture 2 - CSB@Pitt
... Most widely used genome sequence is filtered for repeats • hg38.fa.gz - "Soft-masked" assembly sequence in one file. Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. • hg38.fa.masked.gz - "Hard-ma ...
... Most widely used genome sequence is filtered for repeats • hg38.fa.gz - "Soft-masked" assembly sequence in one file. Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. • hg38.fa.masked.gz - "Hard-ma ...
Pfu DNA Polymerase - G
... thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This mea ...
... thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This mea ...
File
... The fluid material of the nucleus is surrounded by the familiar double membrane called the nuclear envelope. DNA, chromatin and chromosomes can be found inside the nucleus. Chromatin is DNA that is combined with proteins, including histone and non-histone proteins. Chromatin is coiled into larger, h ...
... The fluid material of the nucleus is surrounded by the familiar double membrane called the nuclear envelope. DNA, chromatin and chromosomes can be found inside the nucleus. Chromatin is DNA that is combined with proteins, including histone and non-histone proteins. Chromatin is coiled into larger, h ...
molecular biology
... While majority of the enzymes have highly specific recognition sequence, some of these may be relatively less specific. For example, the recognition sequence of an enzyme may have a purine (A or G) at a place or it may have a pyrimidine (C or T), some times it may be any of the four bases. Further, ...
... While majority of the enzymes have highly specific recognition sequence, some of these may be relatively less specific. For example, the recognition sequence of an enzyme may have a purine (A or G) at a place or it may have a pyrimidine (C or T), some times it may be any of the four bases. Further, ...
Genetic Code, RNA and Protein Synthesis
... transcription. With one or two deletions, the portion of the gene transcribed past the point of the deletion was nonsense. With three deletions, correct gene transcription was restored. This is known as frame-shift alterations of the genetic code. They also looked at the possibility that each code w ...
... transcription. With one or two deletions, the portion of the gene transcribed past the point of the deletion was nonsense. With three deletions, correct gene transcription was restored. This is known as frame-shift alterations of the genetic code. They also looked at the possibility that each code w ...
Biotechnology - GriffinScienceGCM
... Gel Electrophoresis and Southern Blotting • One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis • This technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size, electrical charge, and other properties • A current is applied that cause ...
... Gel Electrophoresis and Southern Blotting • One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis • This technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size, electrical charge, and other properties • A current is applied that cause ...
The Play is the thing… - Biology Learning Center
... • Transcription – 1 strand => new RNA – => new protein ...
... • Transcription – 1 strand => new RNA – => new protein ...
Microbial Genetics (Kroening)
... and for practical applications (biotechnology) in diverse areas of life sciences. Microbiology and microbial genetics are now in the exciting era of “genomic” and “post-genomic” analysis. Complete genome sequences (genetic blueprints) are now being solved at astonishing rates and these hold remarkab ...
... and for practical applications (biotechnology) in diverse areas of life sciences. Microbiology and microbial genetics are now in the exciting era of “genomic” and “post-genomic” analysis. Complete genome sequences (genetic blueprints) are now being solved at astonishing rates and these hold remarkab ...
Drug Target Discovery by Genome Analysis
... Probabilistic methods are best, but can be slow and difficult to use Rigorous are good when used on a small subset of sequences, but too slow to search large sequence database Heuristic methods are the best place to start ...
... Probabilistic methods are best, but can be slow and difficult to use Rigorous are good when used on a small subset of sequences, but too slow to search large sequence database Heuristic methods are the best place to start ...
Many transcription factors recognize DNA shape
... enhancers identifies non-coding variants most likely 239 Predicted HAR Enhancers to affect gene regulation and the targeted genes. functional genomics data - Massive integration of Heart (28) enables cell type specific predictions - Many enhancer-like regions are minimally active and not consistentl ...
... enhancers identifies non-coding variants most likely 239 Predicted HAR Enhancers to affect gene regulation and the targeted genes. functional genomics data - Massive integration of Heart (28) enables cell type specific predictions - Many enhancer-like regions are minimally active and not consistentl ...
Supplementary material S1, S2 (doc 572K)
... between 1 and 3 mM) and TSA (IC50 values between 1 and 3 nM) between the ER- positive breast carcinoma cell line MCF-7, and the ER- negative cell lines MDA MB231 and HeLa. Moreover, at 40 hours after the addition of VPA, around 25% of cells were found to have a DNA content less than in Go/G1 (figu ...
... between 1 and 3 mM) and TSA (IC50 values between 1 and 3 nM) between the ER- positive breast carcinoma cell line MCF-7, and the ER- negative cell lines MDA MB231 and HeLa. Moreover, at 40 hours after the addition of VPA, around 25% of cells were found to have a DNA content less than in Go/G1 (figu ...
Antisense RNA
... • 2. The siRNA-Dicer complex recruits additional components to form an RNA-Induced Silencing Complex (RISC). The siRNA unwinds. • 3. The unwound siRNA base pairs with complementary mRNA, thus guiding the RNAi machinery to the target mRNA. • 4. The target mRNA is effectively cleaved and subsequently ...
... • 2. The siRNA-Dicer complex recruits additional components to form an RNA-Induced Silencing Complex (RISC). The siRNA unwinds. • 3. The unwound siRNA base pairs with complementary mRNA, thus guiding the RNAi machinery to the target mRNA. • 4. The target mRNA is effectively cleaved and subsequently ...
Text Book of Molecular Biology
... chromatin are histones. They are small basic proteins which bind tightly to DNA. There are five families of histones:H1 ,H2A,H2B,H3 and H4. H2A,H2B,H3 and H4 are known as core histones. Two molecules of each families of core histones : (H2A)2 (H2B)2(H3)2(H4)2 , make up the octameric histone core. P ...
... chromatin are histones. They are small basic proteins which bind tightly to DNA. There are five families of histones:H1 ,H2A,H2B,H3 and H4. H2A,H2B,H3 and H4 are known as core histones. Two molecules of each families of core histones : (H2A)2 (H2B)2(H3)2(H4)2 , make up the octameric histone core. P ...
Annotation of Drosophila
... Phase: Number of bases between the complete codon and the splice site Donor phase: Number of bases between the end of the last complete codon and the splice donor site (GT/GC) Acceptor phase: Number of bases between the splice acceptor site (AG) and the start of the first complete codon ...
... Phase: Number of bases between the complete codon and the splice site Donor phase: Number of bases between the end of the last complete codon and the splice donor site (GT/GC) Acceptor phase: Number of bases between the splice acceptor site (AG) and the start of the first complete codon ...
Proteogenomics - The Fenyo Lab
... heavily on the quality of the protein sequence database (DB) • DBs with missing peptide sequences will fail to identify the corresponding peptides • DBs that are too large will have low sensitivity • Ideal DB is complete and small, containing all proteins in the sample and no irrelevant sequences ...
... heavily on the quality of the protein sequence database (DB) • DBs with missing peptide sequences will fail to identify the corresponding peptides • DBs that are too large will have low sensitivity • Ideal DB is complete and small, containing all proteins in the sample and no irrelevant sequences ...
Document
... • You can either (1) align the sequences at the DNA level and then translate to protein sequences, or (2) translate the DNA sequences to protein sequences and then get the alignment. • Try both. Which one gives better results? ...
... • You can either (1) align the sequences at the DNA level and then translate to protein sequences, or (2) translate the DNA sequences to protein sequences and then get the alignment. • Try both. Which one gives better results? ...
Chapter 11 Nucleic Acids Nucleotides
... • Very compact and folded • E. coli DNA is 1.6 mm long, but the E. coli cell is only 0.002 mm long ...
... • Very compact and folded • E. coli DNA is 1.6 mm long, but the E. coli cell is only 0.002 mm long ...
12_Lecture_Presentation - Cornerstone Charter Academy
... 12.17 Genomics is the scientific study of whole genomes Genomics is the study of an organism’s complete set of genes and their interactions – Initial studies focused on prokaryotic genomes – Many eukaryotic genomes have since been investigated ...
... 12.17 Genomics is the scientific study of whole genomes Genomics is the study of an organism’s complete set of genes and their interactions – Initial studies focused on prokaryotic genomes – Many eukaryotic genomes have since been investigated ...
Nucleic Acids Research
... gene, and a 238 bp fragment containing the 3' noncoding region of this gene. When the 38 hybridizing recombinant phage were analyzed with the 2 smaller probes, 9 hybridized to the coding region probe and 29 hybridized to the A-T rich 3' noncoding region probe. The coding region isolates were further ...
... gene, and a 238 bp fragment containing the 3' noncoding region of this gene. When the 38 hybridizing recombinant phage were analyzed with the 2 smaller probes, 9 hybridized to the coding region probe and 29 hybridized to the A-T rich 3' noncoding region probe. The coding region isolates were further ...
Lecture 35 - University of Virginia, Department of Computer Science
... Jacob and Monod, 1959 • Not so simple: cells in an organism have the same DNA, but do different things – Structural genes: make proteins that make us – Regulator genes: control rate of transcription of other genes The genome contains not only a series of blue-prints, but a coordinated program of pr ...
... Jacob and Monod, 1959 • Not so simple: cells in an organism have the same DNA, but do different things – Structural genes: make proteins that make us – Regulator genes: control rate of transcription of other genes The genome contains not only a series of blue-prints, but a coordinated program of pr ...
The Genetic Code
... Section 13.10: Consensus Sequences Consensus sequences – DNA sequences homologous in different genes of same organism ...
... Section 13.10: Consensus Sequences Consensus sequences – DNA sequences homologous in different genes of same organism ...
Genetically Modified Food: A Review on Mechanism of
... Agrobacterium tumefaciens [3]: Plant transformation mediated by Agrobacterium tumefaciens Virulent Strain of Agrobacterium tumefaciens & Agrobacterium rhizo genes contains a large plasmid known as Ti plasmid. These bacteria possess the ability to transfer T-DNA, Causing the Crown of Gall Disease & G ...
... Agrobacterium tumefaciens [3]: Plant transformation mediated by Agrobacterium tumefaciens Virulent Strain of Agrobacterium tumefaciens & Agrobacterium rhizo genes contains a large plasmid known as Ti plasmid. These bacteria possess the ability to transfer T-DNA, Causing the Crown of Gall Disease & G ...
41. Specific terms of reference for the NCR for drug
... Each National Reference Centre (NRC) must meet both the general and the specific terms of reference. In the specific terms of reference, the NRC tasks dedicated to each selected pathogen or group of pathogens are described. It aims to guarantee the knowledge, the know-how and the epidemiological sur ...
... Each National Reference Centre (NRC) must meet both the general and the specific terms of reference. In the specific terms of reference, the NRC tasks dedicated to each selected pathogen or group of pathogens are described. It aims to guarantee the knowledge, the know-how and the epidemiological sur ...
G - Computational Bioscience Program
... regions in non-homologous proteins These regions (sometimes called domains) often have shared structure and/or function Example: Zinc-finger DNA binding motif How to define them? Consensus sequence Regular expression Profile (probability for each amino acid at each position) ...
... regions in non-homologous proteins These regions (sometimes called domains) often have shared structure and/or function Example: Zinc-finger DNA binding motif How to define them? Consensus sequence Regular expression Profile (probability for each amino acid at each position) ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.