Applied and Environmental Microbiology
... coding regions of both genes. Besides duplication, there could be other explanations for the presence of pcsA; for example, lateral gene transfer between bacteria could have occurred and through recombination mechanisms with the chromosomal gene, the high degree of nucleotide similarity could have b ...
... coding regions of both genes. Besides duplication, there could be other explanations for the presence of pcsA; for example, lateral gene transfer between bacteria could have occurred and through recombination mechanisms with the chromosomal gene, the high degree of nucleotide similarity could have b ...
RNA Class: The Classification
... a new variety which is virus-safe, or suppress the gene expression like cance r. Long non coding RNA The revolution of sequencers changed the world of lncRNA. More and more evidence shows that the dysfunction long non-coding RNA is related to many diseases such as HD, liver cancer and so on. You can ...
... a new variety which is virus-safe, or suppress the gene expression like cance r. Long non coding RNA The revolution of sequencers changed the world of lncRNA. More and more evidence shows that the dysfunction long non-coding RNA is related to many diseases such as HD, liver cancer and so on. You can ...
Vitamin supplementation by gut symbionts ensures metabolic
... symbiotic D. fasciatus reared on their natural diet of cottonseeds. The scatter plot depicts the distribution of aposymbiotic/symbiotic log2 intensity ratio (M-value) versus the log2 average intensity (A-value). Each dot represents a single gene in comparison. The red line indicates M ¼ 0. The upper ...
... symbiotic D. fasciatus reared on their natural diet of cottonseeds. The scatter plot depicts the distribution of aposymbiotic/symbiotic log2 intensity ratio (M-value) versus the log2 average intensity (A-value). Each dot represents a single gene in comparison. The red line indicates M ¼ 0. The upper ...
Maize Metabolic Network Construction and Transcriptome Analysis
... gene products, and metabolites that regulate the development of cellular components, cells, tissues, organs, and physiological manifestations of the biochemical networks in response to various extrinsic and intrinsic signals. Understanding maize metabolism at a systems level requires a multifaceted ...
... gene products, and metabolites that regulate the development of cellular components, cells, tissues, organs, and physiological manifestations of the biochemical networks in response to various extrinsic and intrinsic signals. Understanding maize metabolism at a systems level requires a multifaceted ...
PhoB by Phosphate Stress and Controlled by
... Sequence analysis of the S. meliloti ackA locus. Using transposon Tn5B22 as a promoter probe, we previously identified ackA as one of several S. meliloti genes that are up-regulated in response to Pi stress (52). Identification of the interrupted ackA gene in mutant strain RmMSU4 was accomplished by ...
... Sequence analysis of the S. meliloti ackA locus. Using transposon Tn5B22 as a promoter probe, we previously identified ackA as one of several S. meliloti genes that are up-regulated in response to Pi stress (52). Identification of the interrupted ackA gene in mutant strain RmMSU4 was accomplished by ...
Molecular Basis of Polymorphisms of Human Complement
... types have been characterized (5) by variations in their relative electrophoretic mobilities. An additional common structural polymorphism was identified by Koch and Behrendt (6) based on the reactivity of human C3 with a mouse mAb (HAV 4-1) that detected a genetic variation not associated with any ...
... types have been characterized (5) by variations in their relative electrophoretic mobilities. An additional common structural polymorphism was identified by Koch and Behrendt (6) based on the reactivity of human C3 with a mouse mAb (HAV 4-1) that detected a genetic variation not associated with any ...
Exploring the genomic traits of fungus
... 1: Figure S3A; Additional file 2: Table S2). The T3SSs play crucial role in the virulence of plant and human pathogens [21]. However, their functions in nonpathogenic bacteria are still poorly understood; there are indications that mutation of T3SSs in a plant-growth promoting bacteria P. fluorescen ...
... 1: Figure S3A; Additional file 2: Table S2). The T3SSs play crucial role in the virulence of plant and human pathogens [21]. However, their functions in nonpathogenic bacteria are still poorly understood; there are indications that mutation of T3SSs in a plant-growth promoting bacteria P. fluorescen ...
- Wiley Online Library
... by two distinct promoters. In maize, the longer transcript encodes a transit peptide that targets the enzyme to the chloroplast; the shorter transcript lacks the transit peptide and is localized to the cytosol (Glackin & Grula, 1990; Sheen, 1991). The same gene structure is conserved in a broad rang ...
... by two distinct promoters. In maize, the longer transcript encodes a transit peptide that targets the enzyme to the chloroplast; the shorter transcript lacks the transit peptide and is localized to the cytosol (Glackin & Grula, 1990; Sheen, 1991). The same gene structure is conserved in a broad rang ...
Expression of floral identity genes in Clianthus
... ABC model has been shown to be applicable to a wide range of species (Ng and Yanofsky, 2001; Becker and Theissen, 2003), simultaneous investigation of the expression of these genes during an entire annual cycle in natural conditions is limited to a few studies, including that of Sophora tetraptera, ...
... ABC model has been shown to be applicable to a wide range of species (Ng and Yanofsky, 2001; Becker and Theissen, 2003), simultaneous investigation of the expression of these genes during an entire annual cycle in natural conditions is limited to a few studies, including that of Sophora tetraptera, ...
Data for two plasmid isolation techniques, the rapid alkaline extraction... Nucleic Acids Res. 7: 1513-1523) and the rapid boiling technique...
... Neurospora transformants were observed with both types of plasmid DNA minipreps (Table I). The alkaline extraction preparations proved to be the most effective for transformation. However, in all cases the number of transformants obtained was substantially lower (one-fifth to one-twentieth) than the ...
... Neurospora transformants were observed with both types of plasmid DNA minipreps (Table I). The alkaline extraction preparations proved to be the most effective for transformation. However, in all cases the number of transformants obtained was substantially lower (one-fifth to one-twentieth) than the ...
Molecular cloning and nucleotide sequence of another variant of the
... Nucleotide sequence analysis. Restriction fragments of the 4.5 kb HindIII-Hind111 insert (containing the slt gene) in recombinant plasmid pVGTlO (see Fig. l), were subcloned into M13 mp18 and mp19 replicative-form vectors and into pUC19 and pUC18 plasmids for single- and double-stranded DNA sequenci ...
... Nucleotide sequence analysis. Restriction fragments of the 4.5 kb HindIII-Hind111 insert (containing the slt gene) in recombinant plasmid pVGTlO (see Fig. l), were subcloned into M13 mp18 and mp19 replicative-form vectors and into pUC19 and pUC18 plasmids for single- and double-stranded DNA sequenci ...
Acquisition of 1,000 eubacterial genes physiologically transformed a
... Archaebacterial halophiles (Haloarchaea) are oxygen-respiring heterotrophs that derive from methanogens—strictly anaerobic, hydrogen-dependent autotrophs. Haloarchaeal genomes are known to have acquired, via lateral gene transfer (LGT), several genes from eubacteria, but it is yet unknown how many g ...
... Archaebacterial halophiles (Haloarchaea) are oxygen-respiring heterotrophs that derive from methanogens—strictly anaerobic, hydrogen-dependent autotrophs. Haloarchaeal genomes are known to have acquired, via lateral gene transfer (LGT), several genes from eubacteria, but it is yet unknown how many g ...
The home stretch, a first analysis of the nearly completed genome of
... shotgun-sequencing of the entire genome and The University of Texas-Houston Medical School, which carried out a targeted sequencing strategy of CII. Here we describe our current understanding of the genome when data from both of these groups are combined. Previous work had suggested that the two chr ...
... shotgun-sequencing of the entire genome and The University of Texas-Houston Medical School, which carried out a targeted sequencing strategy of CII. Here we describe our current understanding of the genome when data from both of these groups are combined. Previous work had suggested that the two chr ...
Mammalian Two-Hybrid Assay Kit
... The Stratagene Mammalian Two-Hybrid Assay Kit is a powerful method for detecting protein-protein interactions in vivo in mammalian cells. The twohybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression. The mammalian two-hybrid repo ...
... The Stratagene Mammalian Two-Hybrid Assay Kit is a powerful method for detecting protein-protein interactions in vivo in mammalian cells. The twohybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression. The mammalian two-hybrid repo ...
Lecture II - Baylor School of Engineering & Computer Science
... @bases = (‘A’,’C’,’G’,’T’); #Now print each element of the array in a row print “\nHere are all of the bases: “ , @bases; #This prints out: ‘Here are all of the bases: ACGT’ #But, you can print them out with spaces in between print “\nHere they are with spaces” , “@bases”; ...
... @bases = (‘A’,’C’,’G’,’T’); #Now print each element of the array in a row print “\nHere are all of the bases: “ , @bases; #This prints out: ‘Here are all of the bases: ACGT’ #But, you can print them out with spaces in between print “\nHere they are with spaces” , “@bases”; ...
Document
... a storage media. 1. the DNA code a gene is copied into messenger RNA (mRNA) 2. mRNA is the version of the genetic code translated at the ribosome. 3. the ribosome is made up RNA (ribosomal RNA or rRNA) 4. The individual amino acids are brought to the ribosome, as it reads the mRNA, by molecu ...
... a storage media. 1. the DNA code a gene is copied into messenger RNA (mRNA) 2. mRNA is the version of the genetic code translated at the ribosome. 3. the ribosome is made up RNA (ribosomal RNA or rRNA) 4. The individual amino acids are brought to the ribosome, as it reads the mRNA, by molecu ...
List
... reporter genes. Binding sites should be placed 10 bp upstream of the -35 box to select ZFPs. See pH3U3Zif268 map for example. pH3U3-Zif268 (Plasmid 18046) kanamycin resistance plasmid containing the HIS3/URA3 co-cistronic reporter with a Zif268 binding site (GCGTGGGCG) upstream of the weak promoter ...
... reporter genes. Binding sites should be placed 10 bp upstream of the -35 box to select ZFPs. See pH3U3Zif268 map for example. pH3U3-Zif268 (Plasmid 18046) kanamycin resistance plasmid containing the HIS3/URA3 co-cistronic reporter with a Zif268 binding site (GCGTGGGCG) upstream of the weak promoter ...
Composite Structure of Auxin Response Elements
... To define further the minimal AuxREs in the 72-bp fragment described above, we analyzed subfragments of and sitedirected mutations in D1 and D4 as independent AuxREs isolated from one another. Within the 32-bp D4 fragment, three overlapping oligonucleotides, D4-1, D4-2, and D4-3, were cloned as mult ...
... To define further the minimal AuxREs in the 72-bp fragment described above, we analyzed subfragments of and sitedirected mutations in D1 and D4 as independent AuxREs isolated from one another. Within the 32-bp D4 fragment, three overlapping oligonucleotides, D4-1, D4-2, and D4-3, were cloned as mult ...
Functional genomics analysis of foliar condensed tannin and
... Hybridization was carried out in an automated hybridization station (Tecan, Maennedorf, Switzerland) at 40 °C for 36 h (Sartor et al. 2004) using equal amounts of Cy3- and Cy5-labeled targets. Microarray data analysis The slides were scanned with a GenePix 4000B Scanner (Axon Instruments, Union City ...
... Hybridization was carried out in an automated hybridization station (Tecan, Maennedorf, Switzerland) at 40 °C for 36 h (Sartor et al. 2004) using equal amounts of Cy3- and Cy5-labeled targets. Microarray data analysis The slides were scanned with a GenePix 4000B Scanner (Axon Instruments, Union City ...
Supporting information S1.
... The suicide vector pKNG101 was used to introduce the CAT* reporter gene within the Escherichia coli chromosome (Table S2). This plasmid contains a defective pir minus origin of replication (oriR6K), the strAB genes encoding the streptomycin phospotransferase (SmR) as a positive selection marker and ...
... The suicide vector pKNG101 was used to introduce the CAT* reporter gene within the Escherichia coli chromosome (Table S2). This plasmid contains a defective pir minus origin of replication (oriR6K), the strAB genes encoding the streptomycin phospotransferase (SmR) as a positive selection marker and ...
Can epigenetics explain transgenerational transmission of acquired
... evolution, for others a mind prone to unrestrained flights of imagination, views and doctrines have been attributed to Lamarck that bear no resemblance to his actual writings. Lamarck is still seen as the man talking of giraffes stretching their necks, in spite of the fact that the example occurs on ...
... evolution, for others a mind prone to unrestrained flights of imagination, views and doctrines have been attributed to Lamarck that bear no resemblance to his actual writings. Lamarck is still seen as the man talking of giraffes stretching their necks, in spite of the fact that the example occurs on ...
07 Myint
... such viral pathogens has been achieved by subtractive technologies, allied to amplification methods. The principle of subtraction involves the removal of common sequences in infected and non-infected material with complex sequences. When two samples of broadly similar sequences are compared, the con ...
... such viral pathogens has been achieved by subtractive technologies, allied to amplification methods. The principle of subtraction involves the removal of common sequences in infected and non-infected material with complex sequences. When two samples of broadly similar sequences are compared, the con ...
BioPerf: A Benchmark Suite to Evaluate High-Performance
... Portions of genomes could be seen as genomic entities spawned through some dynamic changes in content and order of the ancestral genome. Certain regions, through selection, are conserved over time. Such genomic portions that are related due to their derivation from the same element in a common ances ...
... Portions of genomes could be seen as genomic entities spawned through some dynamic changes in content and order of the ancestral genome. Certain regions, through selection, are conserved over time. Such genomic portions that are related due to their derivation from the same element in a common ances ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.