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tutorial5_12
tutorial5_12

... • GOMO takes DNA binding motifs to find putative target genes and analyze their associated GO terms. A list of significant GO terms that can be linked to the given motifs will be produced. • GOMO returns a list of GO-terms that are significantly associated with target genes of the motif. • Gene Onto ...
Methods for identifying microRNA binding motifs
Methods for identifying microRNA binding motifs

... Computational miRNA target prediction • Sequence complementarily between 5’ seed of miRNA and 3’ UTR of mRNA • Conservation of the miRNA binding site • Topology of base pairing at 5’ seed region of miRNA • Structural accessibility of target sequences and the flanking regions • Favourable minimum fr ...
Prokaryote -( Wikipedia,)
Prokaryote -( Wikipedia,)

... Prokaryotes have incredibly compact genomes compared to eukaryotes, mostly because prokaryote genes lack introns and large non-coding regions between each gene. Whereas nearly 95% of the human genome does not code for proteins or RNAs or includes a gene promoter, nearly all of the prokaryote genome ...
Ultramer® Oligonucleotides - Integrated DNA Technologies
Ultramer® Oligonucleotides - Integrated DNA Technologies

... of Ultramer Oligonucleotides for de novo gene construction. Many of the published procedures for constructing specific DNA sequences from oligos use relatively short sequences (≤60mer) with overlapping stretches of common sequence that permit annealing, followed by assembly using PCR-based strategie ...
Water, Air, and Soil Pollution: Focus
Water, Air, and Soil Pollution: Focus

... encodes for a protein, CP4-EPSPS that is not affected by glyphosate. Roundup Ready® (RR) soybeans were developed by Monsanto (http://www.monsanto.com). The transfer of transgenic DNA can be caused by pollen flow resulting in hybridization with related species (vertical gene transfer) as reported for ...
Thermo Scientific Verso cDNA Kit
Thermo Scientific Verso cDNA Kit

... Reverse-iT™. Verso can synthesize long cDNA strands, up to 11 kb, at a temperature range of 42°C to 57°C. The recommended amount of total RNA to use is between 1 pg and 1 µg. RNA Priming It is recommended that RNA primers be added to the final 1X reaction as follows: 1 µL of anchored oligo-dT (orang ...
BI0I 121 cel]
BI0I 121 cel]

... Discuss the work of Nienberg, Natthaei, and Khorana in cracking the genetic code; explain the genetic code; identify the molecule that carries codons; Explain the charging of tRNA molecules; discuss the minimal number of different tRNA molecules required; discuss the nature of ‘tRNAactivating’ enzym ...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L

... (Notomi et al. 2000). Moreover, this method can be carried out with simple systems and the LAMP reaction can be monitored in real-time through measurement of turbidity, which is correlated with the production of magnesium pyrophosphate, by means of an inexpensive photometer (Mori et al. 2001). Altho ...
Chapter 20
Chapter 20

... • One method of introducing recombinant DNA into eukaryotic cells is electroporation, applying a brief electrical pulse to create temporary holes in plasma membranes • Alternatively, scientists can inject DNA into cells using microscopically thin needles • Once inside the cell, the DNA is incorpora ...
Trends in Plant Science
Trends in Plant Science

... has generated new insights into how this and later in allelism tests were found to have (Ref. 13). Other research groups have obtained important plant defense response is regulated3. mutations in the same gene, hereafter called similar results, although the specific bZIP proNIM1/NPR1. Subsequent wor ...
Complementary DNA Sequence of a Human Cytoplasmic Actin
Complementary DNA Sequence of a Human Cytoplasmic Actin

... randomly distributed. but rather are clustered in specific regions (see Fig. 2). This suggests that certain segments of the actin sequence may be very crucial for filament formation. The percentage of nucleotides substituted within the coding regions of these sequences is significantly greater than ...
PSI- Genes
PSI- Genes

... When Watson and Crick published the structure of DNA in a short article in 1953 they stated: "It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material." The fact that there are two DNA strands that are mirro ...
Figure S1 The yellow color of the Mimulus lewisii nectar
Figure S1 The yellow color of the Mimulus lewisii nectar

... Mimulus guttatus with the nucmer module from the MUMmer 3.0 package (Kurtz et al. 2004), assuming gene collinearity between M. lewisii and M. guttatus. A customized perl script “MUMmer_parser.pl” (available upon request) was written to connect the SL9 contigs into 14 “pseudoscaffolds” based on the M ...
Statistically Significant Patterns in DNA Sequences
Statistically Significant Patterns in DNA Sequences

... Representation of DNA Sequence Motifs consensus sequence motif subsumes a set of similar/aligned sequences in one string. Example: RARE means “retinoic acid response element”, it binds the heterodimer built from RAR (retinoic acid receptor) and RXR (retinoid X receptor). The motif exists of two “ha ...
PPT - 19thpsalm.org
PPT - 19thpsalm.org

... Translation. To every codon there is a special molecule called tRNA that has that codon on one end and has its associated amino acid attached to the opposite end. A given codon (virtually) always associates with the same amino acid -- across all species. Generally a cell must have at least 30 differ ...
Histidine Biosynthetic Pathway and Genes: Structure
Histidine Biosynthetic Pathway and Genes: Structure

... for the identification and elucidation of a novel regulatory mechanism of gene expression, attenuation (38, 294). This term was proposed in 1974 by Takashi Kasai, then working in Phil Hartman’s laboratory (156). By performing in vivo and in vitro transcription studies with his transducing phages and ...
AIS: Androgen Insensitivity Disorder
AIS: Androgen Insensitivity Disorder

... Androgen receptor is highly conserved in mammals and higher organisms but does not have a direct homolog in more distantly related organisms – Should not be used a to determine speciation events, example, chimpanzee and cattle ...
BLAST
BLAST

... Compares a nucleotide query sequence translated in all reading frames against a protein sequence database. You could use this option to find potential translation products of an unknown nucleotide sequence. Compares a protein query sequence against a nucleotide ...
DNA polymerase active site is highly mutable
DNA polymerase active site is highly mutable

... triphosphates, while taking direction from templates with diverse nucleotide sequences (1). In addition, the active site tends to exclude altered nucleotides produced during cellular metabolism. The overall folding pattern of polymerases resembles the human right hand and contains three distinct sub ...
DATA ENCRYPTION USING BIO MOLECULAR INFORMATION
DATA ENCRYPTION USING BIO MOLECULAR INFORMATION

... from unauthorised users. The worst case of an attack within communication is complete control of the encryption system by illegitimate users. This happens by accessing the encryption algorithm to decrypt the data and access sensitive information. Cryptography relies on uncertainty in encoding the me ...
Molecular cloning and characterization of an acyl
Molecular cloning and characterization of an acyl

... In order to identify genes encoding of FatB thioesterase that are actively expressed in peanut, homologue genes with high identity to putative FatB cDNA sequences from Arachis (Genbank ID: EF117305-EF117309) were screened against full length cDNA libraries from different peanut line ‘06-4104’ tissue ...
A new method for predicting signal sequence cleavage sites
A new method for predicting signal sequence cleavage sites

... through +1. The new amlno acid counts presented in Tables 1 & 2 on is ...
molecular phylogeny of the haplosporidia based on
molecular phylogeny of the haplosporidia based on

... The SSU rRNA genes for most of the haplosporidian species were amplified in the polymerase chain reaction (PCR) using ‘‘universal’’ eukaryotic primers (Medlin et al., 1988) and conditions previously described (Flores et al., 1996). Internal SSU rRNA gene primers Urocomp280SSU (Reece and Stokes, 2003 ...
2015 department of medicine research day
2015 department of medicine research day

... Kaboodrangidaem ...
c - Holterman
c - Holterman

... • The new polypeptide is now floating loose in the cytoplasm if translated by a free ribosme. • It might also be inserted into a membrane, if translated by a ribosome bound to the endoplasmic reticulum. • ***Polypeptides fold spontaneously into their 4 levels of structure (quaternary) , and they spo ...
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Promoter (genetics)



In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.
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