Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
... containing 20 units of T4 RNA Ligase 1 with 40 ng of RNA transcript for 2 hours at 37°C resulted in no detectable degradation of the RNA as determined by gel electrophoresis. DNA Exonuclease Activity: Incubation of 20 units of T4 RNA Ligase 1 with 1 µg of mixed single and double-stranded sonicated 3 ...
... containing 20 units of T4 RNA Ligase 1 with 40 ng of RNA transcript for 2 hours at 37°C resulted in no detectable degradation of the RNA as determined by gel electrophoresis. DNA Exonuclease Activity: Incubation of 20 units of T4 RNA Ligase 1 with 1 µg of mixed single and double-stranded sonicated 3 ...
Expanding and understanding the genetic toolbox of the
... and correct deletion mutants. As shown in Figure 1(A), the integration-strain SSOGOI (S. solfataricus GOI) gave the same specific band as found in the plasmid control pET401, which is the backbone plasmid for gene-targeting constructs. However, this band was absent from the correct knockout strain ...
... and correct deletion mutants. As shown in Figure 1(A), the integration-strain SSOGOI (S. solfataricus GOI) gave the same specific band as found in the plasmid control pET401, which is the backbone plasmid for gene-targeting constructs. However, this band was absent from the correct knockout strain ...
Pupko_pairwise
... Similarity between sequences as a result of common ancestry. VLSPAVKWAKVGAHAAGHG ...
... Similarity between sequences as a result of common ancestry. VLSPAVKWAKVGAHAAGHG ...
pIVEX - ISBG
... • Check for any of the additional restriction sites present in the MCS of pIVEX2.3d or pIVEX2.4d. • Include one of these sites into the forward primer. or • Eliminate the restriction site by mutation (e.g. conservative codon exchange, refer to the literature given at the end of chapter 4.1). or • Pr ...
... • Check for any of the additional restriction sites present in the MCS of pIVEX2.3d or pIVEX2.4d. • Include one of these sites into the forward primer. or • Eliminate the restriction site by mutation (e.g. conservative codon exchange, refer to the literature given at the end of chapter 4.1). or • Pr ...
Cell Differentiation during Sexual Development of the Fungus
... is essential for ascospore wall formation (32). Another example concerns the P. anserina car1 gene, which encodes a peroxisomal membrane protein that is essential for peroxisomal assembly (3). car1 mutants show an impaired caryogamy leading to a sterile phenotype. From these data the link between in ...
... is essential for ascospore wall formation (32). Another example concerns the P. anserina car1 gene, which encodes a peroxisomal membrane protein that is essential for peroxisomal assembly (3). car1 mutants show an impaired caryogamy leading to a sterile phenotype. From these data the link between in ...
Molecular cloning and expression of the male sterility - Funpec-RP
... hybrid seeds, and enhances crop yield. It has been applied in several crops such as rice, wheat, sweet sorghum and Chinese cabbage. The thermo-sensitive phenomenon is found in CMS lines of Brassica campestris L. ssp. pekinensis. At low temperature and certain time ranges, the fertility of CMS lines ...
... hybrid seeds, and enhances crop yield. It has been applied in several crops such as rice, wheat, sweet sorghum and Chinese cabbage. The thermo-sensitive phenomenon is found in CMS lines of Brassica campestris L. ssp. pekinensis. At low temperature and certain time ranges, the fertility of CMS lines ...
PCR - Fort Lewis College
... 2. "Touch-down" PCR - start at high annealing temperature, then decrease annealing temperature in steps to reduce non-specific PCR product. Can also be used to determine DNA sequence of known protein sequence. 3. Nested PCR - use to synthesize more reliable product - PCR using a outer set of primer ...
... 2. "Touch-down" PCR - start at high annealing temperature, then decrease annealing temperature in steps to reduce non-specific PCR product. Can also be used to determine DNA sequence of known protein sequence. 3. Nested PCR - use to synthesize more reliable product - PCR using a outer set of primer ...
Figure 1 - York College of Pennsylvania
... Zebrafish (Danio rerio) as a Proposed Model Organism to Study The Expression of Ankyrin-3 and its Link to Bipolar Disorder http://zdmsociety.org/zebrafish-and-human-disease/ ...
... Zebrafish (Danio rerio) as a Proposed Model Organism to Study The Expression of Ankyrin-3 and its Link to Bipolar Disorder http://zdmsociety.org/zebrafish-and-human-disease/ ...
How Scientists Think
... being in a particular shape; thus, the level of enzyme activity could be used to monitor the degree to which ribonuclease protein successfully achieved the proper catalytic shape. To watch the folding process, one might start with nascent proteins, newly made and not yet folded, or one might choose ...
... being in a particular shape; thus, the level of enzyme activity could be used to monitor the degree to which ribonuclease protein successfully achieved the proper catalytic shape. To watch the folding process, one might start with nascent proteins, newly made and not yet folded, or one might choose ...
Nucleic Acids
... their physical properties and component rRNAs depending on their prokaryotic or eukaryotic origin (Fig. V-6). Because cells contain a large number of these ribosomes, rRNAs account for about 70% of the total cellular RNA. Because of their high concentration and uniform size, rRNAs are also easy to d ...
... their physical properties and component rRNAs depending on their prokaryotic or eukaryotic origin (Fig. V-6). Because cells contain a large number of these ribosomes, rRNAs account for about 70% of the total cellular RNA. Because of their high concentration and uniform size, rRNAs are also easy to d ...
Recombinant DNA Technology
... provided new means to determine the functions of proteins and of individual domains within proteins, revealing a host of unexpected relationships between them. By making available large amounts of any protein, they have shown the way to efficient mass production of protein hormones and vaccines. Fin ...
... provided new means to determine the functions of proteins and of individual domains within proteins, revealing a host of unexpected relationships between them. By making available large amounts of any protein, they have shown the way to efficient mass production of protein hormones and vaccines. Fin ...
RIBOSWITCHES - Creighton Chemistry Webserver
... RNA domains that modulate gene expression in response to metabolite binding ...
... RNA domains that modulate gene expression in response to metabolite binding ...
Slides
... and (due to the evolutionary connection) have similar function The sequence alignment problem is an optimization problem: produce the best alignment according to a scoring function A scoring function provide numeric values for each possible symbol pairing and for gaps in an alignment. ...
... and (due to the evolutionary connection) have similar function The sequence alignment problem is an optimization problem: produce the best alignment according to a scoring function A scoring function provide numeric values for each possible symbol pairing and for gaps in an alignment. ...
The polymorphism in MUC1 gene in Nelore cattle
... and serine residues serve as sites for the O-glycosylation responsible for both positive and negative adhesive properties of the molecule. The proline residues are associated with this role, giving the molecule an extended form and raising it well above the surface of the cell membrane (Patton et al ...
... and serine residues serve as sites for the O-glycosylation responsible for both positive and negative adhesive properties of the molecule. The proline residues are associated with this role, giving the molecule an extended form and raising it well above the surface of the cell membrane (Patton et al ...
Cardiac Energy Dependence on Glucose Increases
... elucidate the cardiac cellular response to increased glucose use in a genetic model of inactivated FA oxidation. Methods and Results-—Metabolomics analysis identified 60 metabolites altered in Acsl1H/ hearts, including 6 related to glucose metabolism and 11 to cysteine and glutathione pathways. Con ...
... elucidate the cardiac cellular response to increased glucose use in a genetic model of inactivated FA oxidation. Methods and Results-—Metabolomics analysis identified 60 metabolites altered in Acsl1H/ hearts, including 6 related to glucose metabolism and 11 to cysteine and glutathione pathways. Con ...
Gene–Nutrition Interaction in Human Performance and Exercise
... the body responds to exercise training from the level of the whole human to cellular, subcellular, and molecular mechanisms, and nutritional intake has been established as a primary factor affecting human performance. In addition, a growing body of research has provided support for the notion that r ...
... the body responds to exercise training from the level of the whole human to cellular, subcellular, and molecular mechanisms, and nutritional intake has been established as a primary factor affecting human performance. In addition, a growing body of research has provided support for the notion that r ...
Characterisation of the Aspergillus niger dapB gene, which encodes
... IV (DPP IV) cleave immediately C-terminal to an Ala or a Pro residue, releasing N-terminal X-Ala or X-Pro dipeptides. Due to their unique structural features, proline residues often protect proteins from degradation, and in some cases even play a role in the regulation of their activation. The DPP I ...
... IV (DPP IV) cleave immediately C-terminal to an Ala or a Pro residue, releasing N-terminal X-Ala or X-Pro dipeptides. Due to their unique structural features, proline residues often protect proteins from degradation, and in some cases even play a role in the regulation of their activation. The DPP I ...
Molecular biology technique (I) Southern/Northern
... incubation with proteinase. • Organic or non-inorganic extraction removes proteins. • DNA is purified from solution by alcohol precipitation. • Visible DNA fibers are removed and suspended in buffer. ...
... incubation with proteinase. • Organic or non-inorganic extraction removes proteins. • DNA is purified from solution by alcohol precipitation. • Visible DNA fibers are removed and suspended in buffer. ...
DNA replication - U of L Class Index
... Researchers have used mammalian proteins and plasmids carrying the SV40 origin to study the molecular mechanisms of DNA replication. ...
... Researchers have used mammalian proteins and plasmids carrying the SV40 origin to study the molecular mechanisms of DNA replication. ...
Emerging real-time PCR applications.
... A recent application of HRM with great promise is DNA methylation analysis10. 2-5% of cytosines in eukaryotic genomes are methylated in the 5-position. Methylation occurs in CpG islands upstream of promoters, where it has a regulatory function. The methylation pattern is preserved throughout cell di ...
... A recent application of HRM with great promise is DNA methylation analysis10. 2-5% of cytosines in eukaryotic genomes are methylated in the 5-position. Methylation occurs in CpG islands upstream of promoters, where it has a regulatory function. The methylation pattern is preserved throughout cell di ...
Lesson Overview
... What role does the ribosome play in assembling proteins? Ribosomes use the sequence of codons in mRNA to assemble amino ...
... What role does the ribosome play in assembling proteins? Ribosomes use the sequence of codons in mRNA to assemble amino ...
Ces locus embedded proteins control the non
... Müller, 2005; Lane and Moore, 2011). Very recently we could show that the enzymatic activity of the Ces-NRPS does not follow the canonical NRPS biosynthesis logic, but represents a novel mechanism of non-ribosomal peptide assembly, by using dipeptides rather than monomers as basic units (Marxen et a ...
... Müller, 2005; Lane and Moore, 2011). Very recently we could show that the enzymatic activity of the Ces-NRPS does not follow the canonical NRPS biosynthesis logic, but represents a novel mechanism of non-ribosomal peptide assembly, by using dipeptides rather than monomers as basic units (Marxen et a ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.