1. introduction - International Journal of Computer Applications
... Tool (PSI-BLAST) is usually the first method of choice for the functional annotation of proteins. We carried out the PSIBLAST analysis on the non-redundant positive dataset of ECM proteins in a manner like leave-one-out cross-validation (LOO CV), with the cut-off E-value (-e option of blastpgp) of 0 ...
... Tool (PSI-BLAST) is usually the first method of choice for the functional annotation of proteins. We carried out the PSIBLAST analysis on the non-redundant positive dataset of ECM proteins in a manner like leave-one-out cross-validation (LOO CV), with the cut-off E-value (-e option of blastpgp) of 0 ...
Phosphoproteomics reveals extensive in vivo phosphorylation of
... to be involved in pre-mRNA splicing, including so-called Ser/Arg-rich (SR) proteins. SR proteins promote both constitutive and alternative splicing and have overlapping but distinct functions. They interact with specific RNA sequences mainly through RNA recognition motif (RRM) domains, sometimes wit ...
... to be involved in pre-mRNA splicing, including so-called Ser/Arg-rich (SR) proteins. SR proteins promote both constitutive and alternative splicing and have overlapping but distinct functions. They interact with specific RNA sequences mainly through RNA recognition motif (RRM) domains, sometimes wit ...
tRNA
... • anticodon arm “reads” mRNA, implements Genetic Code aaRS (aminoacyl tRNA synthetase) • “charge” tRNAs with the appropriate amino acid 22 “coded” amino acids ...
... • anticodon arm “reads” mRNA, implements Genetic Code aaRS (aminoacyl tRNA synthetase) • “charge” tRNAs with the appropriate amino acid 22 “coded” amino acids ...
Where can we find disordered proteins?
... Prediction of disordered binding regions – ANCHOR What discriminates disordered binding regions? • A cannot form enough favorable interactions with their sequential environment • It is favorable for them to interact with a globular protein ...
... Prediction of disordered binding regions – ANCHOR What discriminates disordered binding regions? • A cannot form enough favorable interactions with their sequential environment • It is favorable for them to interact with a globular protein ...
Glycosylation of the capsid proteins of cowpea mosaic virus: a
... To confirm the apparent lack of glycosylation of CPMV capsids, a total sugar analysis was undertaken. It was necessary to perform this analysis on the isolated capsid proteins as the vigorous hydrolysis conditions required released large quantities of ribose from the viral RNA when whole capsids wer ...
... To confirm the apparent lack of glycosylation of CPMV capsids, a total sugar analysis was undertaken. It was necessary to perform this analysis on the isolated capsid proteins as the vigorous hydrolysis conditions required released large quantities of ribose from the viral RNA when whole capsids wer ...
erp013_60_3_combined 709..714 - Journal of Experimental Botany
... The paper by Chatre et al. (2009) in this issue is an excellent example of research uncovering unknown unknowns. Typically, investigations into the mechanics of intracellular protein targeting have been performed using protein biochemistry, but the investigation by Chatre et al. (2009) is not typica ...
... The paper by Chatre et al. (2009) in this issue is an excellent example of research uncovering unknown unknowns. Typically, investigations into the mechanics of intracellular protein targeting have been performed using protein biochemistry, but the investigation by Chatre et al. (2009) is not typica ...
Origin of life on Earth Two approaches: • bottom-up
... RNA (ribonucleic acid) can fulfill both functions: Both meteoritic amino acids and those synthesized in Miller-Urey type experiments tend to be almost racemic mixtures: equal amounts of left-handed and right-handed versions Additionally, the set of 20 amino acids used in biology today is not particu ...
... RNA (ribonucleic acid) can fulfill both functions: Both meteoritic amino acids and those synthesized in Miller-Urey type experiments tend to be almost racemic mixtures: equal amounts of left-handed and right-handed versions Additionally, the set of 20 amino acids used in biology today is not particu ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 12. What is meant by lactose intolerance? How it can be overcome? 13. How the viscosity of starch solution is related to its structure? 14. Give a brief account of cyclodextrins and their use. 15. Explain the biological role of NAD+. 16. What are (i) oxidoreductases (ii) transferases. Give two examp ...
... 12. What is meant by lactose intolerance? How it can be overcome? 13. How the viscosity of starch solution is related to its structure? 14. Give a brief account of cyclodextrins and their use. 15. Explain the biological role of NAD+. 16. What are (i) oxidoreductases (ii) transferases. Give two examp ...
03-1 - Pierce College
... 49. Regarding the Na+-K+ pump mechanism, which is not true? a. Three Na+ bind inside cell and are moved out of cell. b. Two K+ bind outside of cell and are move into cell. c. Na+ binding causes phosphate to be released. 50. Uses concentration gradient of a second substance, such as Na+ or H+, to pow ...
... 49. Regarding the Na+-K+ pump mechanism, which is not true? a. Three Na+ bind inside cell and are moved out of cell. b. Two K+ bind outside of cell and are move into cell. c. Na+ binding causes phosphate to be released. 50. Uses concentration gradient of a second substance, such as Na+ or H+, to pow ...
DNA, and in some cases RNA, is the primary source of heritable
... parts of an RNA transcript, different mRNA's can be produced, each coding for a different protein product. Thus the number of different proteins an organism can produce is much greater than its number of genes. Check out the mRNA processing activity in your online textbook. It will help you understa ...
... parts of an RNA transcript, different mRNA's can be produced, each coding for a different protein product. Thus the number of different proteins an organism can produce is much greater than its number of genes. Check out the mRNA processing activity in your online textbook. It will help you understa ...
Biomolecules and Nanotechnology
... protein of any length. Missense errors, which misread the genetic information and substitute an incorrect amino acid at one position, occur at an average frequency of about 1 in 2,000. For a protein composed of 500 amino acids, one out of four proteins will typically have an error, but nearly every ...
... protein of any length. Missense errors, which misread the genetic information and substitute an incorrect amino acid at one position, occur at an average frequency of about 1 in 2,000. For a protein composed of 500 amino acids, one out of four proteins will typically have an error, but nearly every ...
Biological sequence databases
... examine relatively small (5–25) clusters of proteins that have many more connections between one another than with the rest of the network. They reason that these clusters (motifs) represent the most biologically relevant assemblages of proteins such as those that are involved in processes such as ...
... examine relatively small (5–25) clusters of proteins that have many more connections between one another than with the rest of the network. They reason that these clusters (motifs) represent the most biologically relevant assemblages of proteins such as those that are involved in processes such as ...
Macromolecules PPT
... Function- what are they used for? Food sources- what foods will you find these in? Indicator Tests- what tests do we use to find out if a food ...
... Function- what are they used for? Food sources- what foods will you find these in? Indicator Tests- what tests do we use to find out if a food ...
Extraction of RNA File
... strands of DNA by some enzymes in order to transcription the genetic material from DNA to RNA and used the one strand of DNA as a template creating the m RNA strand by help of RNA polymerase after thtat the mRNA will be leaving the nucleus to cytoplasm where Ribosomes found. 5) The second step inclu ...
... strands of DNA by some enzymes in order to transcription the genetic material from DNA to RNA and used the one strand of DNA as a template creating the m RNA strand by help of RNA polymerase after thtat the mRNA will be leaving the nucleus to cytoplasm where Ribosomes found. 5) The second step inclu ...
The Synthetic Machinery of the Cell
... By convention, the genetic code is interpreted with reference to the sequence of bases on m-RNA. In the genetic code there are four bases - A, U, C, G; 64 possible codons (=44); and 20 amino acids The genetic code is degenerate i.e. One amino acid may be represented by more than one codon Codon AUG ...
... By convention, the genetic code is interpreted with reference to the sequence of bases on m-RNA. In the genetic code there are four bases - A, U, C, G; 64 possible codons (=44); and 20 amino acids The genetic code is degenerate i.e. One amino acid may be represented by more than one codon Codon AUG ...
Proteins and Enzymes (p
... determined by the nucleotide base sequence on the DNA. Every organism has its own DNA, and therefore, every organism has its own unique proteins. The significance of the primary structure is the sequence determines the higher levels of the protein. Changing one amino acid may completely alter the st ...
... determined by the nucleotide base sequence on the DNA. Every organism has its own DNA, and therefore, every organism has its own unique proteins. The significance of the primary structure is the sequence determines the higher levels of the protein. Changing one amino acid may completely alter the st ...
Protein Secondary Structure Prediction
... with the help of tRNA. In prokaryotic cells, which have not partition into nucleus and cytoplasm compartments, mRNA can bind to ribosomes while it is being transcribed from DNA. After a certain amount of time the message degrades into its component nucleotides, usually with the assistance of ribonuc ...
... with the help of tRNA. In prokaryotic cells, which have not partition into nucleus and cytoplasm compartments, mRNA can bind to ribosomes while it is being transcribed from DNA. After a certain amount of time the message degrades into its component nucleotides, usually with the assistance of ribonuc ...
Rabbit anti-Occludin (N-term)
... Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The su ...
... Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The su ...
Intracellular localization and tissue
... 3xHA, triple HA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HEK, human embryonic kidney; His6, hexa-histidine; PAT, protein acyltransferase; RT, reverse transcription; SC, synthetic complete ...
... 3xHA, triple HA; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HEK, human embryonic kidney; His6, hexa-histidine; PAT, protein acyltransferase; RT, reverse transcription; SC, synthetic complete ...
Radixin: cytoskeletal adopter and signaling protein
... proteins to the plasma membrane and are found in other cytoskeletal proteins, such as erythrocyte band 4.1 and talin, and several tyrosine kinases and phosphatases. The last 34 residues of the ∼100 amino acid carboxy-terminal domain (CTD) of radixin consists of a (filamentous) F-actin-binding site w ...
... proteins to the plasma membrane and are found in other cytoskeletal proteins, such as erythrocyte band 4.1 and talin, and several tyrosine kinases and phosphatases. The last 34 residues of the ∼100 amino acid carboxy-terminal domain (CTD) of radixin consists of a (filamentous) F-actin-binding site w ...
DNA to Protein
... Part of the bacterial RNA polymerase that helps it recognize the promoter Released after about 10 nucleotides of RNA are linked together Rejoins with a released RNA polymerase to look for a new promoter ...
... Part of the bacterial RNA polymerase that helps it recognize the promoter Released after about 10 nucleotides of RNA are linked together Rejoins with a released RNA polymerase to look for a new promoter ...
Concept review: Chromatography (applied to protein purification)
... • 1. Cell disruption should be performed at cold temperatures. Keep the sample on ice as much as possible and use chilled solutions. This will decrease the activity of the proteases for the simple reasons that all chemical reactions occur more slowly at low temperature. • 2. Add protease inhibitors ...
... • 1. Cell disruption should be performed at cold temperatures. Keep the sample on ice as much as possible and use chilled solutions. This will decrease the activity of the proteases for the simple reasons that all chemical reactions occur more slowly at low temperature. • 2. Add protease inhibitors ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.