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Cloning of genes from genomic DNA Part 1 and 2: DNA Isolation
Cloning of genes from genomic DNA Part 1 and 2: DNA Isolation

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... features of PIN-domains and summarise the evidence for their biochemical and biological functions. It will then discuss the VapBC TA systems and collect the disparate evidence for their roles as response operons to stressors and the changing environments encountered by the microbes in whose genomes ...
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ASE using Solexa Protocol

... Allele-specific expression using Solexa protocol 1) Identify a SNP between two parental lines. 2) Design gene specific 18-20bp annealing primers as follows: forward primer flanking the 5' end of the SNP such that the base immediately following the 3’ end of the primer is the SNP, the second 200-300b ...
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... (1) Genome is cut into small, overlapping fragments with a restriction enzyme, and each piece is cloned, forming a DNA library. (2) The DNA fragments must overlap other fragments, so the restriction enzyme is not allowed to cut at every possible restriction site. (3) Computers assemble the fragments ...
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http://ict.aiias.edu/vol_26A/26Acc_271-290.pdf

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... reduce water loss and the transformation of the original foliaceous gills to foliaceous lungs or to a tracheal system of aerial breathing”. Words such as “become”, “development”, “transformation”, cause students to think that all these changes are very simple and happened easily. Causative factors ...
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... Blue/White screening (Terminal integrity): A DNA vector is digested at a unique site within lacZα gene with a 10-fold excess of enzyme, and then ligated, transformed and plated on X-gal/IPTG plate. Successful expression of the β-galactosidase indicates that lacZα gene remains integrity after cloning ...
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Silencer (genetics)

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