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Proteins containing unusual amino acid sequences
Proteins containing unusual amino acid sequences

... excluded, the rat and the human protein have more than 90% identity, but the long glutamine runs are at positions 100 residues apart in the sequence, and there are indications that individual humans have different length glutamine runs. In most cases the runs are more pronounced at rhe amino acid ra ...
$doc.title

... presence of various concentrations of either 1,10­ phenanthroline or 1,7-phenanthroline was carried out. ...
tRNA
tRNA

Lecture20_Translation
Lecture20_Translation

Using Genes for Antibiotic Resistance to Trace Sources of Bacterial
Using Genes for Antibiotic Resistance to Trace Sources of Bacterial

... When bacteria become resistant to antibiotics, doctors have very few other ways to fight bacteria and the infections they cause—making antibiotic resistant bacteria a serious problem. Bacteria can become antibiotic resistant when they are continually exposed to antibiotics over a long period of time ...
Southern Blot
Southern Blot

... a. Give the order of the compounds in the metabolic pathway and indicate the specific step that is blocked in each mutant strain. b. Several sets of double mutations were produced. For example, double mutant 1, 2 has mutant forms for enzymes 1 and 2. Pairs of these double mutations were combined in ...
Cholesterol, steroids, and related molecules
Cholesterol, steroids, and related molecules

... response to angiotensin II, a peptide hormone that signals reduced Na+ levels in the blood. StAR gene transcription is blocked in response to atrial natriuretic factor, a signal of elevated Na+ levels. The StAR protein has a short half-life, so transcriptional regulation effectively controls aldoste ...
Lab Dept: Anatomic Pathology Test Name: GENE
Lab Dept: Anatomic Pathology Test Name: GENE

video slide
video slide

... • A restriction enzyme will usually make many cuts in a DNA molecule yielding a set of restriction fragments • The most useful restriction enzymes cut DNA in a staggered way producing fragments with “sticky ends” that can bond with complementary “sticky ends” of other fragments • DNA ligase is an en ...
Southern Blot
Southern Blot

... a. Give the order of the compounds in the metabolic pathway and indicate the specific step that is blocked in each mutant strain. b. Several sets of double mutations were produced. For example, double mutant 1, 2 has mutant forms for enzymes 1 and 2. Pairs of these double mutations were combined in ...
Methods for identifying microRNA binding motifs
Methods for identifying microRNA binding motifs

... Functional importance of miRNA binding in post-transcriptional gene regulation drives effort in identifying conserved binding motifs But non-canonical motifs dominate over those based on seed sequence Experimental approaches based on RISC complex formation and immunoprecipitation help isolate miRNA- ...
BCM 6200 - Purification des proteines membranaires
BCM 6200 - Purification des proteines membranaires

... Genome integration necessary for construct, ie. constructs generated first prior to transforming cells. Zeocin (antibiotic) is expensive for initial steps. Up to 25% false positives (antibiotic resistant but no clone insterted) High pressures (>30 kPSI) are needed to rupture cell membranes due to ce ...
Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes
Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes

... Salmonella spp. None of these enzymes is vital or appears to have a unique value under the anaerobic conditions required for cobalamin synthesis. These enzymes are as follows. (i) Homocysteine methyltransferases catalyze the final step in methionine synthesis. Both a cobalamin-dependent (metH) enzym ...
Mutations, the molecular clock, and models of sequence evolution
Mutations, the molecular clock, and models of sequence evolution

... How do you measure how different two homologous DNA sequences are? Sequence 0 ...
IB Image Review Key
IB Image Review Key

... contact proteins at the promoter, initiating transcription. • Coordinate regulation: Enhancer for liver-specific genes ...
Molecular Analysis of the Clavulanic Acid Regulatory Gene Isolated
Molecular Analysis of the Clavulanic Acid Regulatory Gene Isolated

... genes of the clavulanic acid pathway are within a 12kb EcoRI DNA fragment of the S. clavuligerus genome, because this fragment appears to confer production of clavulanic acid when introduced in Streptomyces lividans (25). Very little is known about the regulation of the genes of the clavulanic acid ...
The biological meaning of pairwise alignments
The biological meaning of pairwise alignments

2 -1 -2 -1 1 2 K
2 -1 -2 -1 1 2 K

...  Their structural units, domains or folds, can be covalently linked to generate multi-domain proteins  Domains are not only structurally, but also functionally, discrete units – domain family members are structurally and functionally conserved and recombined in complex ways during evolution  Doma ...
Recombinant DNA technology
Recombinant DNA technology

... DNA in a process called restriction. The host bacterial DNA itself is methylated by a modification enzyme (a methylase) to be protected from the restriction enzyme’s activity. ...
Self-adaptation of Genome Size in Artificial Organisms | SpringerLink
Self-adaptation of Genome Size in Artificial Organisms | SpringerLink

... where 0 and 1 are the complementary bases. Not all the positions are functional: coding sequences (genes) are detected by transcription-translation process inspired by bacterial genetics. Genes are then translated into basic functional elements (proteins). These elementary functions are combined tog ...
A-Ag
A-Ag

... • 5 classes: IgG, IgA, IgM, IgD, IgE 1b. Immunogen • Protein or a substance coupled to a carrier • When introduced into foreign host -> induce Ab to form 1c. Antigen • Any material which can react with Ab • May not induce Ab formation ...
Alignment: pairs of sequences
Alignment: pairs of sequences

... KQTGKGV ...
File
File

... DNA in a process called restriction. The host bacterial DNA itself is methylated by a modification enzyme (a methylase) to be protected from the restriction enzyme’s activity. ...
Creating the Gene Ontology Resource: Design and Implementation
Creating the Gene Ontology Resource: Design and Implementation

... molecular function, biological process, and cellular component, and providing a community database resource that supports the use of these ontologies. The GO Consortium was initiated by scientists associated with three model organism databases: SGD, the Saccharomyces Genome database; FlyBase, the Dr ...
video slide - Buena Park High School
video slide - Buena Park High School

... – It continues to untwist the double helix, exposing about 10 to 20 DNA bases at a time for pairing with RNA nucleotides ...
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Silencer (genetics)

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