Making worms that glow in the dark
... fluorescent GFP molecules within the bodies of the transgenic worms. In the same year, some other scientists performed the same experiment in E. coli. These results paved the way for the use of GFP as a “reporter molecule” in a wide variety of living organisms. Since 1994, the use of GFP has explode ...
... fluorescent GFP molecules within the bodies of the transgenic worms. In the same year, some other scientists performed the same experiment in E. coli. These results paved the way for the use of GFP as a “reporter molecule” in a wide variety of living organisms. Since 1994, the use of GFP has explode ...
CSIR researchers have proved it`s possible to manipulate bacterial
... functional (Crampton et al 2007). Two advantages of developing the Grampositive flagellin display system are that Gram-positive bacteria are more robust than their Gram-negative counterparts and the chimeric flagella are easily isolated from the cell surface. In this study we wanted to determine lim ...
... functional (Crampton et al 2007). Two advantages of developing the Grampositive flagellin display system are that Gram-positive bacteria are more robust than their Gram-negative counterparts and the chimeric flagella are easily isolated from the cell surface. In this study we wanted to determine lim ...
Journal of Molecular Microbiology and Biotechnology
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
... mycosubtilin biosynthesis gene cluster devoted to synthesis of an iturin-like compound, is situated at the same location [Duitman et al., 1999], suggesting that additional NRPS operons could be integrated in different ways in this area either as an insertion or as a substitution of existing NRPS ope ...
Basics of Protein Expression
... • Cloning in E. coli, then transfect DNA into yeast and screen for stable integration into yeast chromosome • Time required for selection of high expressing clones • Once established, rapid growth in shake flasks or ...
... • Cloning in E. coli, then transfect DNA into yeast and screen for stable integration into yeast chromosome • Time required for selection of high expressing clones • Once established, rapid growth in shake flasks or ...
Additional File 1
... it performs worse than RIDDLE and components for disjoint KEGG sets (C). For disjoint and time-split GO sets, the method is bested by RIDDLE and diffusion-based components (D,E). ...
... it performs worse than RIDDLE and components for disjoint KEGG sets (C). For disjoint and time-split GO sets, the method is bested by RIDDLE and diffusion-based components (D,E). ...
How Can Transposons Accelerate Your Genomics
... – Note: if your desired gene is not represented in the final transposed library, successful insertion may have created a lethal mutation. – Run a control transposition reaction in high-efficiency TransforMax™ EC100 Electrocompetent E. coli (>109 cfu/µg). ...
... – Note: if your desired gene is not represented in the final transposed library, successful insertion may have created a lethal mutation. – Run a control transposition reaction in high-efficiency TransforMax™ EC100 Electrocompetent E. coli (>109 cfu/µg). ...
Document
... enzyme and stretch the active site out of shape • phosphates and other factors can pull on another part of the enzyme to pull the active site into the correct shape ...
... enzyme and stretch the active site out of shape • phosphates and other factors can pull on another part of the enzyme to pull the active site into the correct shape ...
Promega Notes: Separate Isolation of Genomic DNA and Total RNA
... Analysis of RNA is important for many studies including cellular development, responses to environmental stimuli and disease states. Promega's SV Total RNA Isolation System provides a rapid and safe method for the purification of high quality total RNA. The SV RNA System is based on a silica membran ...
... Analysis of RNA is important for many studies including cellular development, responses to environmental stimuli and disease states. Promega's SV Total RNA Isolation System provides a rapid and safe method for the purification of high quality total RNA. The SV RNA System is based on a silica membran ...
Chapter 9 Review Questions 1. Please state two different types of
... 1. Please state two different types of cues that can lead to DNA damage. Which of these cues would spontaneous DNA damage fall under? Which of these cues would induced DNA damage fall under? Please explain your answer in detail. 2. Please state 3 external agents that lead to DNA damage. 3. Please ex ...
... 1. Please state two different types of cues that can lead to DNA damage. Which of these cues would spontaneous DNA damage fall under? Which of these cues would induced DNA damage fall under? Please explain your answer in detail. 2. Please state 3 external agents that lead to DNA damage. 3. Please ex ...
Word Pro - Sezutsu.lwp
... gland may be used to produce novel types of silks. To date, Ayf and spider silk proteins have been expressed in this way (I. Kobayashi & K. Kojima, pers. commun.). Since the silk of Rhodinia fugax has an interesting appearance, we decided to identify the fibroin gene of this species. Rhodinia fugax ...
... gland may be used to produce novel types of silks. To date, Ayf and spider silk proteins have been expressed in this way (I. Kobayashi & K. Kojima, pers. commun.). Since the silk of Rhodinia fugax has an interesting appearance, we decided to identify the fibroin gene of this species. Rhodinia fugax ...
Biological Polymers - McQuarrie General Chemistry
... earth originated from l-amino acids; and once the process started, it continued to use only l-isomers, which, unlike the d -isomers, are recognized by our enzymes. This has great significance in biochemistry. For example, the antibiotic penicillin attacks proteins containing d -alanine that are foun ...
... earth originated from l-amino acids; and once the process started, it continued to use only l-isomers, which, unlike the d -isomers, are recognized by our enzymes. This has great significance in biochemistry. For example, the antibiotic penicillin attacks proteins containing d -alanine that are foun ...
enzymes and vectors
... • The choice of a vector depends on the design of the experimental system and how the cloned gene will be screened or utilized subsequently • Most vectors contain a prokaryotic origin of replication allowing maintenance in bacterial cells. • Some vectors contain an additional eukaryotic origin of re ...
... • The choice of a vector depends on the design of the experimental system and how the cloned gene will be screened or utilized subsequently • Most vectors contain a prokaryotic origin of replication allowing maintenance in bacterial cells. • Some vectors contain an additional eukaryotic origin of re ...
Lecture 12
... • The two SH3 domains fold with similar rates, as do ADAh2 and acyl phosphatase, suggesting that protein topology is an important factor in determining the rate of protein folding. • Examples of low and high contact order (sequence separation between residues that physically contact in the native ...
... • The two SH3 domains fold with similar rates, as do ADAh2 and acyl phosphatase, suggesting that protein topology is an important factor in determining the rate of protein folding. • Examples of low and high contact order (sequence separation between residues that physically contact in the native ...
Genome Sequence of an Extremely Halophilic Archaeon
... was the finding of an extremely acidic complement of encoded proteins, which is likely directly related to protein function in its hypersaline (>4M KCl) cytoplasm (11). Calculated isoelectric points (pIs) for predicted proteins showed an average pI of approx 5, a prediction confirmed by proteomic an ...
... was the finding of an extremely acidic complement of encoded proteins, which is likely directly related to protein function in its hypersaline (>4M KCl) cytoplasm (11). Calculated isoelectric points (pIs) for predicted proteins showed an average pI of approx 5, a prediction confirmed by proteomic an ...
Proteins
... Globins (hemoglobin (Hb), Mb; carry di oxygen) Cytochromes (Fe in a porphorin ring; carry electrons) albumins (serum; carry fatty acids) immunoglobulins (antibodies, defense) receptor proteins (receive and respond to molecular signals) Genetic regulatory proteins regulate when, how, and to what exte ...
... Globins (hemoglobin (Hb), Mb; carry di oxygen) Cytochromes (Fe in a porphorin ring; carry electrons) albumins (serum; carry fatty acids) immunoglobulins (antibodies, defense) receptor proteins (receive and respond to molecular signals) Genetic regulatory proteins regulate when, how, and to what exte ...
Structural Location of Disease-Associated Single Nucleotide
... In this study we systematically examine positions of nsSNPs in motif regions of proteins. For this purpose, we use the PFAM database of probabilistic models of protein domains and families derived using the HMM method 33. PFAM has been used extensively in many bioinformatics studies and has played a ...
... In this study we systematically examine positions of nsSNPs in motif regions of proteins. For this purpose, we use the PFAM database of probabilistic models of protein domains and families derived using the HMM method 33. PFAM has been used extensively in many bioinformatics studies and has played a ...
Disease name
... noted in the field, the relationship between these cases and herpesvirus infection was analyzed out in this study. Three DNA fragments were cloned from diseased abalone. Amino acid analysis revealed the 1380-bp sequence has 38% (8/21) identity to human herpesvirus-7 DNA polymerase catalytic subunit, ...
... noted in the field, the relationship between these cases and herpesvirus infection was analyzed out in this study. Three DNA fragments were cloned from diseased abalone. Amino acid analysis revealed the 1380-bp sequence has 38% (8/21) identity to human herpesvirus-7 DNA polymerase catalytic subunit, ...
Slideshow presentation (Microsoft PowerPoint) (PPT
... silencing immune suppressive genes, which can be used for anti-cancer therapy, vaccine development. 3. Down-regulating immune responses through silencing immune responsive genes possesses therapeutic potential in treatments of autoimmune and allergic diseases as well as graft rejection in ...
... silencing immune suppressive genes, which can be used for anti-cancer therapy, vaccine development. 3. Down-regulating immune responses through silencing immune responsive genes possesses therapeutic potential in treatments of autoimmune and allergic diseases as well as graft rejection in ...