91.510_ch8_part1
... Definition of a motif A motif (or fingerprint) is a short, conserved region of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. PROSITE (www.expasy.org/prosite) is ...
... Definition of a motif A motif (or fingerprint) is a short, conserved region of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. PROSITE (www.expasy.org/prosite) is ...
The alphabet soup of plant intracellular signalling: enter cyclic
... before the pathway itself has been defined either biochemically, or by molecular genetics in plants. A foretaste is provided by a quick scan of the TIGR Arabidopsis gene index [35] which reveals four hits when searched for RyR, 251 with protein kinase A and 217 with protein kinase C. Naturally, DNA ...
... before the pathway itself has been defined either biochemically, or by molecular genetics in plants. A foretaste is provided by a quick scan of the TIGR Arabidopsis gene index [35] which reveals four hits when searched for RyR, 251 with protein kinase A and 217 with protein kinase C. Naturally, DNA ...
the genetic and cytogenetic localization of the three structural genes
... WOLFEand AKAM1977). These proteins are being studied in order to understand the mechanism of their control. As part of this program, we have mapped the coding sequence for LSP-2 to 37.0 and 6832-4 on chromosome 3 (AKAM et al. 1978) ;in this paper, we describe the genetics of the coding sequences for ...
... WOLFEand AKAM1977). These proteins are being studied in order to understand the mechanism of their control. As part of this program, we have mapped the coding sequence for LSP-2 to 37.0 and 6832-4 on chromosome 3 (AKAM et al. 1978) ;in this paper, we describe the genetics of the coding sequences for ...
Bioinformatics and Functional Genomics, Chapter 8, Part 1
... Definition of a motif A motif (or fingerprint) is a short, conserved region of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. PROSITE (www.expasy.org/prosite) is ...
... Definition of a motif A motif (or fingerprint) is a short, conserved region of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. PROSITE (www.expasy.org/prosite) is ...
Composite Structure of Auxin Response Elements
... assays. The unmutated 72-bp fragment was induced greater than fivefold by the addition of 50 pM a-naphthalene acetic acid (NAA). Two base pair substitutions within the 5‘ region of D4 revealed two domains important for maximal auxin inducibility (Figure 2A). Mutations M1, M2, and M4 within an 8-bp e ...
... assays. The unmutated 72-bp fragment was induced greater than fivefold by the addition of 50 pM a-naphthalene acetic acid (NAA). Two base pair substitutions within the 5‘ region of D4 revealed two domains important for maximal auxin inducibility (Figure 2A). Mutations M1, M2, and M4 within an 8-bp e ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... that is known to inhibit protein synthesis in yeast cell-free systems. The ribosomes were then subjected to chemical modification, in both the absence and the presence of cycloheximide, using either dimethyl sulphate or kethoxal. The experimental conditions that were used were essentially as describ ...
... that is known to inhibit protein synthesis in yeast cell-free systems. The ribosomes were then subjected to chemical modification, in both the absence and the presence of cycloheximide, using either dimethyl sulphate or kethoxal. The experimental conditions that were used were essentially as describ ...
PCR: Polymerase Chain Reaction
... opposing strains sequence and extend primers sequence to match (complementary). Taq polymerase is used because it can withstand high temperature necessary for DNA strand separation and can be left in reaction to attach and start copying the template. The result is two new helixes in place of the fir ...
... opposing strains sequence and extend primers sequence to match (complementary). Taq polymerase is used because it can withstand high temperature necessary for DNA strand separation and can be left in reaction to attach and start copying the template. The result is two new helixes in place of the fir ...
Recombinant DNA Technology
... 4. transformation: DNA manipulated in vitro can be put back into the living cells by a simple process . – The transformed DNA replicates and expresses its genes. ...
... 4. transformation: DNA manipulated in vitro can be put back into the living cells by a simple process . – The transformed DNA replicates and expresses its genes. ...
Concept 14.4: Translation is the RNA
... stretches of nucleotides that lie between coding regions The noncoding regions are called intervening sequences, or introns The other regions are called exons and are usually translated into amino acid sequences RNA splicing removes introns and joins exons, creating an mRNA molecule with a con ...
... stretches of nucleotides that lie between coding regions The noncoding regions are called intervening sequences, or introns The other regions are called exons and are usually translated into amino acid sequences RNA splicing removes introns and joins exons, creating an mRNA molecule with a con ...
475 S07 background questions
... Explain the relationship between differentiation and differential gene expression. Describe at what level gene expression is generally controlled. Explain how DNA methylation and histone acetylation affect chromatin structure and the regulation of transcription. Define epigenetic inheritance. Descri ...
... Explain the relationship between differentiation and differential gene expression. Describe at what level gene expression is generally controlled. Explain how DNA methylation and histone acetylation affect chromatin structure and the regulation of transcription. Define epigenetic inheritance. Descri ...
Isolation of insertion elements from Gram
... in question into E. coli and by testing for sucrose sensitivity (data not shown). Therefore, besides mutation of the sacB gene, at least one other, so far unknown resistance mechanism should exist. Since in E. coli the lethal effect parallels with an active levan sucrase in the periplasm [2], chromo ...
... in question into E. coli and by testing for sucrose sensitivity (data not shown). Therefore, besides mutation of the sacB gene, at least one other, so far unknown resistance mechanism should exist. Since in E. coli the lethal effect parallels with an active levan sucrase in the periplasm [2], chromo ...
University of Groningen Expression and engineering of
... unit was sufficient to restore mRNA accumulation to wild-type levels (Chapter 4), while one intron in the hph-gene (1020 bp) was not sufficient to reach maximal accumulation. This suggests that more than one intron is needed for efficient expression of genes encoding long transcripts. The data also ...
... unit was sufficient to restore mRNA accumulation to wild-type levels (Chapter 4), while one intron in the hph-gene (1020 bp) was not sufficient to reach maximal accumulation. This suggests that more than one intron is needed for efficient expression of genes encoding long transcripts. The data also ...
Predicting functional linkages from gene fusions with
... topoisomerase proteins, are linked due to the presence of the yeast topoisomeraseII (Top2) protein, which is a fusion of both gyrase A-like and gyrase B-like domains. To apply orthology to predict precise linkages between these proteins, the fusion protein must first be separated into domains, and t ...
... topoisomerase proteins, are linked due to the presence of the yeast topoisomeraseII (Top2) protein, which is a fusion of both gyrase A-like and gyrase B-like domains. To apply orthology to predict precise linkages between these proteins, the fusion protein must first be separated into domains, and t ...
Chapter 20
... • One method of introducing recombinant DNA into eukaryotic cells is electroporation, applying a brief electrical pulse to create temporary holes in plasma membranes • Alternatively, scientists can inject DNA into cells using microscopically thin needles • Once inside the cell, the DNA is incorpora ...
... • One method of introducing recombinant DNA into eukaryotic cells is electroporation, applying a brief electrical pulse to create temporary holes in plasma membranes • Alternatively, scientists can inject DNA into cells using microscopically thin needles • Once inside the cell, the DNA is incorpora ...
Journal of Bacteriology
... Mass spectrometric (MS) analysis. (i) MALDI-TOF MS. Polyketides of B. amyloliquefaciens FZB 42 were identified in extracts of lyophilized culture filtrates and XAD7 extracts by MALDI-TOF MS. Mass spectra were recorded with a Bruker Daltonik Reflex MALDI-TOF instrument containing a 337-nm nitrogen la ...
... Mass spectrometric (MS) analysis. (i) MALDI-TOF MS. Polyketides of B. amyloliquefaciens FZB 42 were identified in extracts of lyophilized culture filtrates and XAD7 extracts by MALDI-TOF MS. Mass spectra were recorded with a Bruker Daltonik Reflex MALDI-TOF instrument containing a 337-nm nitrogen la ...
Aptamers as Drugs. PDF
... a wide variety of medical diseases and conditions, as well as improved methods for identifying such drugs. Advances The ability to expand the functional groups available to aptamers through the incorporation of a variety of chemical moieties could potentially open up new and improved chemistry and b ...
... a wide variety of medical diseases and conditions, as well as improved methods for identifying such drugs. Advances The ability to expand the functional groups available to aptamers through the incorporation of a variety of chemical moieties could potentially open up new and improved chemistry and b ...
Exercise 8
... is one mechanism by which bacteria transfer genes between contemporaries, allowing the phenomenon of “lateral gene transfer;” this is thought to be a major mechanism by which antibiotic resistance has become widespread among many different types of bacteria. Genes carried on relatively small circula ...
... is one mechanism by which bacteria transfer genes between contemporaries, allowing the phenomenon of “lateral gene transfer;” this is thought to be a major mechanism by which antibiotic resistance has become widespread among many different types of bacteria. Genes carried on relatively small circula ...
A1981LF07700001
... that I received my postdoctoral training. "In the 1960s the emphasis was on the 'mechanism' of phytochrome action, i.e., the biophysical and molecular steps leading from the formation of active phytochrome to the final displays (photoresponses). As a convenient guiding principle in studies about the ...
... that I received my postdoctoral training. "In the 1960s the emphasis was on the 'mechanism' of phytochrome action, i.e., the biophysical and molecular steps leading from the formation of active phytochrome to the final displays (photoresponses). As a convenient guiding principle in studies about the ...
The Human Cell Poster Advertisements
... estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, alternative splicing of the messenger RNA. Posttranslational modification of the nascent protein, such as phosphorylation and gl ...
... estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, alternative splicing of the messenger RNA. Posttranslational modification of the nascent protein, such as phosphorylation and gl ...
Complete nucleotide sequences of two soybean
... soybean lines (Buzzel & Tu, 1984; Chen et al., 1988; Cho & Goodman, 1979) and transmission by aphid species (Lucas & Hill, 1980). Like other potyviruses, SMV genomic RNA encodes a large precursor polyprotein that is processed by a virusencoded protease(s) (Ghabrial et al., 1990) to yield several pro ...
... soybean lines (Buzzel & Tu, 1984; Chen et al., 1988; Cho & Goodman, 1979) and transmission by aphid species (Lucas & Hill, 1980). Like other potyviruses, SMV genomic RNA encodes a large precursor polyprotein that is processed by a virusencoded protease(s) (Ghabrial et al., 1990) to yield several pro ...
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... and the receptors used for its detection. In gram negative bacteria typically use Lux I/R quorum-sensing (Yang, ...
... and the receptors used for its detection. In gram negative bacteria typically use Lux I/R quorum-sensing (Yang, ...
Library subtraction of in vitro cDNA libraries to identify differentially
... extraction and ethanol precipitation, the DNA was dissolved in 50 /tl 5 mM HEPES, 1 mM EDTA, pH 7.5, and boiled for 5 min. The DNA was cooled and mixed with 50 /tl photo-probe biotin (Vector Labs, Burlingame, CA), irradiated with a sunlamp, and processed as described (1). 15 /tg of the biotinylated ...
... extraction and ethanol precipitation, the DNA was dissolved in 50 /tl 5 mM HEPES, 1 mM EDTA, pH 7.5, and boiled for 5 min. The DNA was cooled and mixed with 50 /tl photo-probe biotin (Vector Labs, Burlingame, CA), irradiated with a sunlamp, and processed as described (1). 15 /tg of the biotinylated ...
The codon adaptation index-a measure of directional synonymous
... conparison between species because the proportional division of the codon table into the two categories may differ; e.g., Ikemura (1) identified 21 optimal codons for 14 amino acids in E.coll. and 19 optimal codons for 13 amino acids in yeast. Gribskov et al. (10) have recently proposed another inde ...
... conparison between species because the proportional division of the codon table into the two categories may differ; e.g., Ikemura (1) identified 21 optimal codons for 14 amino acids in E.coll. and 19 optimal codons for 13 amino acids in yeast. Gribskov et al. (10) have recently proposed another inde ...