Root Hair Genes - PBL Technology
... surface area of the plant’s root system, playing important roles in plant anchorage, water use and nutrient acquisition. rhd6 mutants of Arabidopsis are “root hair deficient”. The John Innes group cloned this gene and a family of related “RHD6-like” RSL genes and characterised their expression and e ...
... surface area of the plant’s root system, playing important roles in plant anchorage, water use and nutrient acquisition. rhd6 mutants of Arabidopsis are “root hair deficient”. The John Innes group cloned this gene and a family of related “RHD6-like” RSL genes and characterised their expression and e ...
Lab 1 - DNA Isolation from Drosophila melanogaster (Fly DNA Mini
... 1. Freeze 50 Drosophila larvae or flies in a microcentrifuge Tube P (pellet) on dry ice or wet ice to slow down the fly movement. ...
... 1. Freeze 50 Drosophila larvae or flies in a microcentrifuge Tube P (pellet) on dry ice or wet ice to slow down the fly movement. ...
DNA and RNA:
... dogma. The first step of the central dogma is called transcription. Transcription uses DNA as a template to copy genetic information into the form of RNA. In turn, RNA is involved in translation. Translation synthesizes the protein using RNA as a template (figure 7.1). Understanding how the steps of ...
... dogma. The first step of the central dogma is called transcription. Transcription uses DNA as a template to copy genetic information into the form of RNA. In turn, RNA is involved in translation. Translation synthesizes the protein using RNA as a template (figure 7.1). Understanding how the steps of ...
Transcript
... DNA replication is a process that all cells must go through prior to any type of cell division. When cells replicate their DNA, they make two identical copies of their DNA from the original copy. This process assures that the daughter cells resulting from cell division will each have a complete copy ...
... DNA replication is a process that all cells must go through prior to any type of cell division. When cells replicate their DNA, they make two identical copies of their DNA from the original copy. This process assures that the daughter cells resulting from cell division will each have a complete copy ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previously shown that differential transcription of the chlamydial major outer membrane protein gene, ompl, occurs over the time course of the developmental cycle: transcrip ...
... Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previously shown that differential transcription of the chlamydial major outer membrane protein gene, ompl, occurs over the time course of the developmental cycle: transcrip ...
Construction of Recombinant Expression Vectors to Study the Effect
... The pET-32 vector series is designed for high-level expression of recombinant thioredoxin fusion proteins. Previous studies have shown that linkage to thioredoxin increases the yield of biologically active proteins expressed in Escherichia coli. How thioredoxin promotes protein solubility is not kno ...
... The pET-32 vector series is designed for high-level expression of recombinant thioredoxin fusion proteins. Previous studies have shown that linkage to thioredoxin increases the yield of biologically active proteins expressed in Escherichia coli. How thioredoxin promotes protein solubility is not kno ...
Polymerase Chain Reaction
... PCR amplification CONTAMINATION WITH GENOMIC DNA. DNAse treat RNA prior to experiment, use primers either side of introns, and do control reaction WITHOUT reverse transcriptase. ...
... PCR amplification CONTAMINATION WITH GENOMIC DNA. DNAse treat RNA prior to experiment, use primers either side of introns, and do control reaction WITHOUT reverse transcriptase. ...
One, Two, Three: Polycomb Proteins Hit All Dimensions of
... of WUS expression [61]. The MADS box transcription factor, SHORT VEGETATIVE PHASE (SVP), and the GRAS transcription factor, SCARECROW (SCR), also physically interact with LHP1 and bind to specific LHP1 target loci, and thus could participate in the recruitment of PRC complexes and in the maintenance ...
... of WUS expression [61]. The MADS box transcription factor, SHORT VEGETATIVE PHASE (SVP), and the GRAS transcription factor, SCARECROW (SCR), also physically interact with LHP1 and bind to specific LHP1 target loci, and thus could participate in the recruitment of PRC complexes and in the maintenance ...
Downlod - nimelssa unimaid
... The only way to improve resolving power is to reduce substantially the wavelength of the light. This is achieved by the electromagnetic beam of the electron microscope. The beam is focused through the object suspended on its metal grid, and is magnified before striking a fluorescent screen to be tra ...
... The only way to improve resolving power is to reduce substantially the wavelength of the light. This is achieved by the electromagnetic beam of the electron microscope. The beam is focused through the object suspended on its metal grid, and is magnified before striking a fluorescent screen to be tra ...
Production of industrially relevant compounds in prokaryotic
... is catechol. In some examples, the 3,4-DHB decarboxylase gene is from Klebsiella pneumoniae, Enlerobacler cloacae, Laclobacillus planlarum, or Closlridium bulryricum (for example, one of SEQ ID NOs: 4-11). [0009] In a further embodiment, the method includes expressing a heterologous asbF gene, a het ...
... is catechol. In some examples, the 3,4-DHB decarboxylase gene is from Klebsiella pneumoniae, Enlerobacler cloacae, Laclobacillus planlarum, or Closlridium bulryricum (for example, one of SEQ ID NOs: 4-11). [0009] In a further embodiment, the method includes expressing a heterologous asbF gene, a het ...
Chapter 8
... Initiation factors are required for all stages of initiation, including binding the initiator tRNA, 40S subunit attachment to mRNA, movement along the mRNA, and joining of the 60S subunit. Eukaryotic initiator tRNA is a Met-tRNA that is different from the Met-tRNA used in elongation, but the methion ...
... Initiation factors are required for all stages of initiation, including binding the initiator tRNA, 40S subunit attachment to mRNA, movement along the mRNA, and joining of the 60S subunit. Eukaryotic initiator tRNA is a Met-tRNA that is different from the Met-tRNA used in elongation, but the methion ...
Problem of Focus - Clarkson University
... The first step in proposed research is to grow bacteria with the plasmid and express the protein. This first step is called transformation where the plasmid is put into a special strain of bacteria. The next step is induction of the plasmid to produce the protein. To visualize the protein one must u ...
... The first step in proposed research is to grow bacteria with the plasmid and express the protein. This first step is called transformation where the plasmid is put into a special strain of bacteria. The next step is induction of the plasmid to produce the protein. To visualize the protein one must u ...
PPT File
... The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. The total number of genes is estimated at 30,000 ...
... The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. The total number of genes is estimated at 30,000 ...
PhoB by Phosphate Stress and Controlled by
... and the dct genes, which are required for uptake of dicarboxylic acids (malate and/or succinate), the primary sources of energy for the bacteria during symbiosis (reviewed in references 13, 33, and 51). In different rhizobia, two-component regulatory systems have been shown to be involved in the pos ...
... and the dct genes, which are required for uptake of dicarboxylic acids (malate and/or succinate), the primary sources of energy for the bacteria during symbiosis (reviewed in references 13, 33, and 51). In different rhizobia, two-component regulatory systems have been shown to be involved in the pos ...
T. congolense - Centre for Genomic Research
... Hepcidin in turn is regulated by IL6. Splenic IL6 production increases most in C57BL/6 post infection, concomitant with this, hepcidin expression increases approximately twofold post infection and by more in C57BL/6 than AJ or BALB/c. However ferroportin transcription also increases but by more in A ...
... Hepcidin in turn is regulated by IL6. Splenic IL6 production increases most in C57BL/6 post infection, concomitant with this, hepcidin expression increases approximately twofold post infection and by more in C57BL/6 than AJ or BALB/c. However ferroportin transcription also increases but by more in A ...
Applied and Environmental Microbiology
... Symbiotic phenotypes of the citrate synthase mutants of R. tropici. Figure 4 shows the nodulation kinetics of the different citrate synthase mutants compared with the wild type. Both the chromosomal mutant (CFNE 150) and the double mutant (CFNE 160) have a large reduction in nodule number per plant; ...
... Symbiotic phenotypes of the citrate synthase mutants of R. tropici. Figure 4 shows the nodulation kinetics of the different citrate synthase mutants compared with the wild type. Both the chromosomal mutant (CFNE 150) and the double mutant (CFNE 160) have a large reduction in nodule number per plant; ...
Pfu DNA Polymerase - G
... thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This mea ...
... thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This mea ...
Comparative study of pathogenesis-related protein - NOPR
... Pathogenesis-related protein 5 (PR5) is a family of proteins that are induced by different phytopathogens in many plants and share significant sequence similarity with thaumatin. In the present study, 8 putative PR5 genes were cloned from different species of Zingiberaceae, viz., Alpinia luteocarpa, ...
... Pathogenesis-related protein 5 (PR5) is a family of proteins that are induced by different phytopathogens in many plants and share significant sequence similarity with thaumatin. In the present study, 8 putative PR5 genes were cloned from different species of Zingiberaceae, viz., Alpinia luteocarpa, ...
Bacteriophages use an expanded genetic code on
... RF0 IodoY. a, Schematic showing the possible effects of an amber codon on peptides detected by a mass spectrometry-based proteomics analysis. Termination at the amber codon or incorporation of 3iodotyrosine or a canonical amino acid results in different masses for the directly informative peptides. ...
... RF0 IodoY. a, Schematic showing the possible effects of an amber codon on peptides detected by a mass spectrometry-based proteomics analysis. Termination at the amber codon or incorporation of 3iodotyrosine or a canonical amino acid results in different masses for the directly informative peptides. ...
Managing people in sport organisations: A strategic
... genes are bright red, and VSG pseudogenes are pink. The large dots at the end of the chromosome represent telomeres. Green arrows are VSG promoters. The Xs represent recombination or gene conversion events. ESAG are Expression Site Associated Genes, non-VSG genes, which are part of the polycistronic ...
... genes are bright red, and VSG pseudogenes are pink. The large dots at the end of the chromosome represent telomeres. Green arrows are VSG promoters. The Xs represent recombination or gene conversion events. ESAG are Expression Site Associated Genes, non-VSG genes, which are part of the polycistronic ...
Dicot and monocot plants differ in retinoblastoma
... genes, and the STRECHER program (http://bioweb.pasteur.fr/ seqanal/interfaces/stretcher.html) was used to identify the borders. The regions containing RBR-like protein sequences from the A pocket domain up to the C-terminus were aligned by using ClustalW (http://bioweb.pasteur.fr/seqanal/ClustalW.ph ...
... genes, and the STRECHER program (http://bioweb.pasteur.fr/ seqanal/interfaces/stretcher.html) was used to identify the borders. The regions containing RBR-like protein sequences from the A pocket domain up to the C-terminus were aligned by using ClustalW (http://bioweb.pasteur.fr/seqanal/ClustalW.ph ...
