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... Selectable trait that enables E. coli that carry the plasmid to be separated from E. coli that do not (e.g., antibiotic resistance, grow cells on antibiotic; only those cells with the anti-biotic resistance grow in colony). ...
... Selectable trait that enables E. coli that carry the plasmid to be separated from E. coli that do not (e.g., antibiotic resistance, grow cells on antibiotic; only those cells with the anti-biotic resistance grow in colony). ...
A machine learning approach to gene expression data analysis
... The better accuracy of the RS ensemble does not simply depend on the better accuracy of their component base learners CIBB-WIRN 2004 ...
... The better accuracy of the RS ensemble does not simply depend on the better accuracy of their component base learners CIBB-WIRN 2004 ...
Characterization of Ubiquitin/Proteasome
... their protein products encoded in S. cerevisiae’s genome to those of higher eukaryotes make yeast a time and money efficient avenue for gaining insight into cellular mechanisms of higher organisms. Over the years, the worth of S. cerevisiae has been further established, with it acting as a valuable ...
... their protein products encoded in S. cerevisiae’s genome to those of higher eukaryotes make yeast a time and money efficient avenue for gaining insight into cellular mechanisms of higher organisms. Over the years, the worth of S. cerevisiae has been further established, with it acting as a valuable ...
Review Article The biosafety of molecular farming in plants
... differences are particularly evident in yeast and insect cells but occur even when murine cells are used as expression hosts. The diversity of plant-based expression systems Various plant-based expression technologies can be used for molecular farming, but in most cases the production crop is genera ...
... differences are particularly evident in yeast and insect cells but occur even when murine cells are used as expression hosts. The diversity of plant-based expression systems Various plant-based expression technologies can be used for molecular farming, but in most cases the production crop is genera ...
Identification and characterization of the ergochrome gene cluster in
... and the strain was cultured under growth conditions unfavorable for the pigment production. After 7 days of cultivation the culture was clearly pigmented. However, in contrast to the OE TF cultures the color was more purple red (Fig. 6). The UV signal of clavorubin at 22.8 min in Fig. 9 highlights ...
... and the strain was cultured under growth conditions unfavorable for the pigment production. After 7 days of cultivation the culture was clearly pigmented. However, in contrast to the OE TF cultures the color was more purple red (Fig. 6). The UV signal of clavorubin at 22.8 min in Fig. 9 highlights ...
DNA Structure & Function
... floating in the cytoplasm in a region of the cell known as the nucleoid This chromosome is usually supercoiled folding over on itself like a twisted rubberband Prokaryotes DO NOT have introns, which we will discuss in greater detail later ...
... floating in the cytoplasm in a region of the cell known as the nucleoid This chromosome is usually supercoiled folding over on itself like a twisted rubberband Prokaryotes DO NOT have introns, which we will discuss in greater detail later ...
Sequence Alignment Techniques
... • Multiple alignment illustrates relationships between two or more sequences • When the sequences involved are diverse, the conserved residues are often key residues associated with maintenance of structural stability or biological function • Multiple alignments can reveal many clues about protein s ...
... • Multiple alignment illustrates relationships between two or more sequences • When the sequences involved are diverse, the conserved residues are often key residues associated with maintenance of structural stability or biological function • Multiple alignments can reveal many clues about protein s ...
PLoS Pathogens
... experimentally confirmed T3SEs were collected from the 23 species having at least one characterized T3SE (Table S1). Using a combination of BLASTP, TBLASTX, and pair-wise BLAST (BL2SEQ), we identified two common and interrelated features among T3SEs from all species. First, the N or C terminus of many ...
... experimentally confirmed T3SEs were collected from the 23 species having at least one characterized T3SE (Table S1). Using a combination of BLASTP, TBLASTX, and pair-wise BLAST (BL2SEQ), we identified two common and interrelated features among T3SEs from all species. First, the N or C terminus of many ...
Exonuclease active site: a more complete description
... interacts with the hydroxyl of Y165, with the catalytic aspartate of the exonuclease III motif (D169), and with the scissile phosphate through a water-mediated hydrogen bond (Figure S1). The quality of the maps for the former conformation of copy B is extremely poor due to disorder, so only the mor ...
... interacts with the hydroxyl of Y165, with the catalytic aspartate of the exonuclease III motif (D169), and with the scissile phosphate through a water-mediated hydrogen bond (Figure S1). The quality of the maps for the former conformation of copy B is extremely poor due to disorder, so only the mor ...
PROTEIN SYNTHESIS
... ovalburain (egg white) and casein, a major protein found in milk. Proteins are macromolecules (polypeptides) containing atoms of carbon, hydrogen, oxygen, nitrogen, and often sulfur. Trace amounts of phosphorus, copper, and zinc may also be found. Proteins are composed of a linear arrangement of ami ...
... ovalburain (egg white) and casein, a major protein found in milk. Proteins are macromolecules (polypeptides) containing atoms of carbon, hydrogen, oxygen, nitrogen, and often sulfur. Trace amounts of phosphorus, copper, and zinc may also be found. Proteins are composed of a linear arrangement of ami ...
Document
... - essential to keep matured chloroplast active under fluctuating environmental conditions (day/night, light stress, temperature ..) ...
... - essential to keep matured chloroplast active under fluctuating environmental conditions (day/night, light stress, temperature ..) ...
RNA Extraction SOP
... II. Determining the Correct Amount of Starting Material The RNA content varies greatly between bacterial strains and under different growth conditions: 1x 109 bacteria correspond to approximately 20-160 µg of total RNA. It is essential to use the correct number of bacteria in order to obtain optimal ...
... II. Determining the Correct Amount of Starting Material The RNA content varies greatly between bacterial strains and under different growth conditions: 1x 109 bacteria correspond to approximately 20-160 µg of total RNA. It is essential to use the correct number of bacteria in order to obtain optimal ...
Systems Biology of Biological Nitrogen Fixation
... meliloti fail to grow on TCA cycle intermediates or pyruvate [23]. Additionally, the transcript of one of several genes designated in the R. etli genome as phosphoglycerate mutase (in this case pgm,) was also detected by transcriptomics. A notable metabolic feature in nodule bacteria was the activit ...
... meliloti fail to grow on TCA cycle intermediates or pyruvate [23]. Additionally, the transcript of one of several genes designated in the R. etli genome as phosphoglycerate mutase (in this case pgm,) was also detected by transcriptomics. A notable metabolic feature in nodule bacteria was the activit ...
... iii) Why are transport proteins (e.g. K+ channel) considered (at least by me) to be enzymes, even though they do not change the chemical structure of the substrate? (1 pt) i) Good substrates have many interactions with the active site, leading to small off-rates. Therefore the substrate is bound lon ...
Supplementary materials
... 3. Identification of gene duplications: Alignments of the Shewanella proteins were generated using Darwin 2.0 (6). Proteins were aligned over at least 83 amino acids or ≥70% of the sequence lengths. Strain specific duplications were identified from the data set as the proteins that had a better matc ...
... 3. Identification of gene duplications: Alignments of the Shewanella proteins were generated using Darwin 2.0 (6). Proteins were aligned over at least 83 amino acids or ≥70% of the sequence lengths. Strain specific duplications were identified from the data set as the proteins that had a better matc ...
FEBS Letters
... (table 2) suggest that proteins b and c are more related with each other than with protein e. (ii) Alternatively,prote~ e can be completely unrelated to proteins b and c and the strong similarity between their amino termini [ 161 might be related to one or more properties which these 3 proteins shar ...
... (table 2) suggest that proteins b and c are more related with each other than with protein e. (ii) Alternatively,prote~ e can be completely unrelated to proteins b and c and the strong similarity between their amino termini [ 161 might be related to one or more properties which these 3 proteins shar ...
PDF
... genes have extensive similarity (BLASTP; 1e-10) to those of B. Thirty-seven families of protein-coding repetitive sehalodurans. Their overall genome similarity ranks the highest quences longer than 300 bp were also categorized. Most of among all the sequenced genomes, regardless if they are therthem ...
... genes have extensive similarity (BLASTP; 1e-10) to those of B. Thirty-seven families of protein-coding repetitive sehalodurans. Their overall genome similarity ranks the highest quences longer than 300 bp were also categorized. Most of among all the sequenced genomes, regardless if they are therthem ...
The Production, Purification, and Bioactivity of Recombinant Bovine
... since they resemble each other in inferred amino acid sequence more than they do the related bovine IFNaM (5). There is particularly high conservation of the 3'untranslated ends of bTP-1 and oTP-1 cDNA, which is not extended to bovine IFNa,, cDNA (5). Finally, the organization of up-stream promoter ...
... since they resemble each other in inferred amino acid sequence more than they do the related bovine IFNaM (5). There is particularly high conservation of the 3'untranslated ends of bTP-1 and oTP-1 cDNA, which is not extended to bovine IFNa,, cDNA (5). Finally, the organization of up-stream promoter ...
Feb 24 exam all parts
... d) What vitamin is necessary for effective blood clotting _______Vit K__________ (1 mark) e) What special behaviour is associated with this amino acid, and how does it promote activation of prothrombin on negative phospholipid or glass surfaces? (3 marks) ...
... d) What vitamin is necessary for effective blood clotting _______Vit K__________ (1 mark) e) What special behaviour is associated with this amino acid, and how does it promote activation of prothrombin on negative phospholipid or glass surfaces? (3 marks) ...
Incomplete handout (Lecture 2) - the Conway Group
... on the requirement that it forms a Watson-‐Crick base pair with the DNA strand that is being transcribed (the template strand -‐ only one strand of DNA is transcribed at a time). ...
... on the requirement that it forms a Watson-‐Crick base pair with the DNA strand that is being transcribed (the template strand -‐ only one strand of DNA is transcribed at a time). ...
Expression of floral identity genes in Clianthus
... stamens, and C genes alone specifies carpels (Bowman et al., 1991; Coen and Meyerowitz, 1991). This designation ...
... stamens, and C genes alone specifies carpels (Bowman et al., 1991; Coen and Meyerowitz, 1991). This designation ...