The QIAexpressionist™
... High-level expression of 6xHis-tagged proteins in E. coli using the QIAexpress pQE vectors is based on the T5 promoter transcription–translation system. pQE plasmids belong to the pDS family of plasmids (Bujard et al. 1987) and were derived from plasmids pDS56/RBSII and pDS781/RBSII-DHFRS (Stüber et ...
... High-level expression of 6xHis-tagged proteins in E. coli using the QIAexpress pQE vectors is based on the T5 promoter transcription–translation system. pQE plasmids belong to the pDS family of plasmids (Bujard et al. 1987) and were derived from plasmids pDS56/RBSII and pDS781/RBSII-DHFRS (Stüber et ...
Lecture 27
... • DNA strands are simultaneously replicated. • Takes place at replication fork - junction where the two parental DNA are pried apart and where the two daughter strands are synthesized. • Leading strand is continuously copied from the 3’ to 5’ parental template in the 5’ to 3’ direction • Lagging str ...
... • DNA strands are simultaneously replicated. • Takes place at replication fork - junction where the two parental DNA are pried apart and where the two daughter strands are synthesized. • Leading strand is continuously copied from the 3’ to 5’ parental template in the 5’ to 3’ direction • Lagging str ...
RNA Extraction SOP
... 18. To elute, add 50 µl of RNase-free water directly onto the RNeasy silica-gel membrane. 19. Close the tube gently, and centrifuge for 1 minute at 11,000 rpm. 20. If the expected RNA yield is >30 µg, repeat the elution in steps 18 and 19 with a second volume of RNase-free water. Elute in the same c ...
... 18. To elute, add 50 µl of RNase-free water directly onto the RNeasy silica-gel membrane. 19. Close the tube gently, and centrifuge for 1 minute at 11,000 rpm. 20. If the expected RNA yield is >30 µg, repeat the elution in steps 18 and 19 with a second volume of RNase-free water. Elute in the same c ...
Dynamics of the trp Operon
... * Cluster of genes controlled by a single (?) feedback regulatory mechanism. ...
... * Cluster of genes controlled by a single (?) feedback regulatory mechanism. ...
PowerPoint 演示文稿
... residue becomes ubiquitinylated by covalent linkage of molecules of the small, highly conserved, ubiquitin, via its C-terminal Gly, to lysine residues in the protein. The ubiquitinylated protein is digested by a 26S protease complex in a reaction that requires ATP and releases intact ubiquitin for r ...
... residue becomes ubiquitinylated by covalent linkage of molecules of the small, highly conserved, ubiquitin, via its C-terminal Gly, to lysine residues in the protein. The ubiquitinylated protein is digested by a 26S protease complex in a reaction that requires ATP and releases intact ubiquitin for r ...
Chapter 5 Gases
... • Operon: group of genes together with a promoter–operator DNA sequence that controls their transcription – Although first discovered in bacteria, operons also occur in archaea and eukaryotes ...
... • Operon: group of genes together with a promoter–operator DNA sequence that controls their transcription – Although first discovered in bacteria, operons also occur in archaea and eukaryotes ...
Taq polymerase errors in PCR: Frequency and management
... • Well studied by Chen and colleagues (1991, Mutation Research) • Topic: mutations in the human adenine phosphoribosyltransferase genes = HPTR deficiency that cause a kidney disease 1. Cloning and sequencing of HPRT gene from human DNA library (reference sequence) 2. Cloning of HPRT PCR products and ...
... • Well studied by Chen and colleagues (1991, Mutation Research) • Topic: mutations in the human adenine phosphoribosyltransferase genes = HPTR deficiency that cause a kidney disease 1. Cloning and sequencing of HPRT gene from human DNA library (reference sequence) 2. Cloning of HPRT PCR products and ...
RNAi
... • GAL4 is a transcriptional activator from yeast that recognizes a DNA sequence called the UAS (upstream activating sequence) • We can use this to control expression of YFG in a tissue specific manner by using enhancer elements specific for the tissue we are interested in ...
... • GAL4 is a transcriptional activator from yeast that recognizes a DNA sequence called the UAS (upstream activating sequence) • We can use this to control expression of YFG in a tissue specific manner by using enhancer elements specific for the tissue we are interested in ...
The Protein Truncation Test
... The detection method for PTT products must be considered when designing primers for amplification (18). Typically, [35S]methionine is the label of choice but other labels such as [35S]cysteine and [3H]leucine could be used as well. Thus, the amplified segments should contain one or more of these amino ...
... The detection method for PTT products must be considered when designing primers for amplification (18). Typically, [35S]methionine is the label of choice but other labels such as [35S]cysteine and [3H]leucine could be used as well. Thus, the amplified segments should contain one or more of these amino ...
Gene Section Transcription 3) Atlas of Genetics and Cytogenetics
... Phosphorylation leads to dimerization of STAT3 via intermolecular pTyr-SH2 interactions. STAT3 can also heterodimerize with STAT1. (Recent data suggests that STAT3 can possibly form a dimmer without tyrosine phosphorylation and that phosphorylation leads to changes dimmer conformation). Tyrosine of ...
... Phosphorylation leads to dimerization of STAT3 via intermolecular pTyr-SH2 interactions. STAT3 can also heterodimerize with STAT1. (Recent data suggests that STAT3 can possibly form a dimmer without tyrosine phosphorylation and that phosphorylation leads to changes dimmer conformation). Tyrosine of ...
HUMAN PRIMARY CELLS RNA PRODUCTS Total RNA
... Q. Is there micro RNA in the Total RNA products? A. No, there is no micro RNA in the Total RNA products. However, AllCells Comprehensive RNA products contain the small and micro RNA. Q. What should I expect in terms of quality and integrity of the total RNA? A. At AllCells, we analyze the RNA qualit ...
... Q. Is there micro RNA in the Total RNA products? A. No, there is no micro RNA in the Total RNA products. However, AllCells Comprehensive RNA products contain the small and micro RNA. Q. What should I expect in terms of quality and integrity of the total RNA? A. At AllCells, we analyze the RNA qualit ...
Manual: Universal Human miRNA Reference RNA
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
Special Study Project III
... 34. Which of the following is not a goal of biotechnology? a. To understand more about the process of inheritance and gene expression b. To provide better understanding and treatment of various human diseases c. To generate improved agricultural plants and domestic animals d. To prevent the inherit ...
... 34. Which of the following is not a goal of biotechnology? a. To understand more about the process of inheritance and gene expression b. To provide better understanding and treatment of various human diseases c. To generate improved agricultural plants and domestic animals d. To prevent the inherit ...
Transcription Translation PowerPoint
... 3. mRNA is complementary to the ____ DNA strand so it has the same nucleotide sequence as the ____ DNA strand. 4. Every three nucleotides on mRNA is called a ____. (You may need to come back for this) ...
... 3. mRNA is complementary to the ____ DNA strand so it has the same nucleotide sequence as the ____ DNA strand. 4. Every three nucleotides on mRNA is called a ____. (You may need to come back for this) ...
Presentation 1 Guidelines
... An example is the –35 and –10 consensus sequence found in bacterial promoters. At –35, it is TTGACA, but it can differ by one or two nucleotides and still function efficiently as a promoter. In the consensus sequences within bacterial promoters, the –35 site is primarily for recognition by sigma fac ...
... An example is the –35 and –10 consensus sequence found in bacterial promoters. At –35, it is TTGACA, but it can differ by one or two nucleotides and still function efficiently as a promoter. In the consensus sequences within bacterial promoters, the –35 site is primarily for recognition by sigma fac ...
SURVEY AND SUMMARY Nucleolar responses to DNA double
... ber of DSBs (28), thus suggesting that NHEJ is the predominant repair pathway for DSBs in rDNA as it is for DSBs in the rest of the genome (5). The precise localization of DSB repair by NHEJ in rDNA is not yet clear. 53BP1 localizes to nucleolar caps and associates with DSBs but factors like ku80 an ...
... ber of DSBs (28), thus suggesting that NHEJ is the predominant repair pathway for DSBs in rDNA as it is for DSBs in the rest of the genome (5). The precise localization of DSB repair by NHEJ in rDNA is not yet clear. 53BP1 localizes to nucleolar caps and associates with DSBs but factors like ku80 an ...
B3.3 Genetics ANSWERS Worksheet Two Molecular Genetics 1
... Transcription makes a copy of the code by producing mRNA with RNA nucleotides. Whereas DNA replication uses DNA nucleotides to produce an identical copy. DNA replication uses both sides of the DNA, whereas transcription only uses the coding strand. The enzymes are also different; DNA replication use ...
... Transcription makes a copy of the code by producing mRNA with RNA nucleotides. Whereas DNA replication uses DNA nucleotides to produce an identical copy. DNA replication uses both sides of the DNA, whereas transcription only uses the coding strand. The enzymes are also different; DNA replication use ...
Magnesium chloride hexahydrate Product Number - Sigma
... nucleic acid structure.1,2 Magnesium is an essential co-factor in many enzymes, including deoxyribonuclease (DNAse), the restriction enzymes EcoR I and EcoR V, and Ribonuclease H.3,4 Magnesium also stabilizes polymeric nucleic acids such as transfer RNA and ribozymes.5 Conditions for optimal use of ...
... nucleic acid structure.1,2 Magnesium is an essential co-factor in many enzymes, including deoxyribonuclease (DNAse), the restriction enzymes EcoR I and EcoR V, and Ribonuclease H.3,4 Magnesium also stabilizes polymeric nucleic acids such as transfer RNA and ribozymes.5 Conditions for optimal use of ...
Study Guide B
... RNA polymerase A large transcription complex, including RNA polymerase and other proteins, assembles at the start of a gene and begins to unwind the DNA. Using one strand of the DNA as a template, RNA polymerase strings together a complementary strand of RNA. The RNA strand detaches from the DNA as ...
... RNA polymerase A large transcription complex, including RNA polymerase and other proteins, assembles at the start of a gene and begins to unwind the DNA. Using one strand of the DNA as a template, RNA polymerase strings together a complementary strand of RNA. The RNA strand detaches from the DNA as ...
Designing and making sgRNA constructs
... ‘G’ base at the transcription start site. Hence, we recommend finding a 20bp genome target starting with the base ‘G’. If you have to use other bases at the starting position of your genome target, you could add an additional ‘G’ to the front of your target. If you are going to use the construct sim ...
... ‘G’ base at the transcription start site. Hence, we recommend finding a 20bp genome target starting with the base ‘G’. If you have to use other bases at the starting position of your genome target, you could add an additional ‘G’ to the front of your target. If you are going to use the construct sim ...
Document
... Slide 1 – Gene Expression Part 3 Slide 2 - Eukaryotic Vs. Prokaryotic Transcription Presence of a nucleus in eukaryotes o means transcription & translation occur in separate compartments of cell o all the translation occur outside in the cytoplasm o in prokaryotes it could happen at the same time, ...
... Slide 1 – Gene Expression Part 3 Slide 2 - Eukaryotic Vs. Prokaryotic Transcription Presence of a nucleus in eukaryotes o means transcription & translation occur in separate compartments of cell o all the translation occur outside in the cytoplasm o in prokaryotes it could happen at the same time, ...
Protein Translation
... RNA Structure Depends on Sequence A can pair with U and the C with G via hydrogen bonding just as with DNA. Secondary RNA structure is critical in how it performs its function. RNA Structure and RNA Sequence enable an RNA to interact specifically with proteins. ...
... RNA Structure Depends on Sequence A can pair with U and the C with G via hydrogen bonding just as with DNA. Secondary RNA structure is critical in how it performs its function. RNA Structure and RNA Sequence enable an RNA to interact specifically with proteins. ...
FoldNucleus: web server for the prediction of RNA
... The server was used to determine the folding nuclei for 21 proteins. A comparison of the calculation results with the experimental data shows that the model provides good A-value predictions for protein structures determined by X-ray analysis with consideration of hydrogen atoms and, less successful ...
... The server was used to determine the folding nuclei for 21 proteins. A comparison of the calculation results with the experimental data shows that the model provides good A-value predictions for protein structures determined by X-ray analysis with consideration of hydrogen atoms and, less successful ...
