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Document
Document

... Functional genomics is a field of molecular biology that uses the vast wealth of data produced by genomic projects (such as genome sequencing projects) to describe gene (and protein) functions and interactions. Functional genomics attempts to answer questions about the function of DNA at the levels ...
exBI6102 Introductory Bioinformatics
exBI6102 Introductory Bioinformatics

... This subject teaches basic bioinformatics concepts, databases, tools and applications. It is meant for students to apply their computational knowledge into one of the most promising industries, life science. Upon the completion of this subject, the students should be able to ...
protein synthesis TEACHER
protein synthesis TEACHER

... The Genetic Code • A codon designates an amino acid • An amino acid may have more than one codon • There are 20 amino acids, but 64 possible codons • Some codons tell the ribosome to stop translating copyright cmassengale ...
Document
Document

... • GFP is a visual marker • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development • Formation of different organs • Screenable marker to identify transgenic organisms ...
Cross-species gene transfer: a major factor in evolution?
Cross-species gene transfer: a major factor in evolution?

... Gilbert 25, 26 has suggested that the functional role of introns is evolutionary; they create new arrangements of protein functional domains that result in new proteins. Thus, introns evolved in the course of evolving new proteins; otherwise, they have no necessary role to play in gene expression o ...
DNA
DNA

... conservative theory. The experiment was repeated but this time the bacteria was able to replicate twice. If replication was dispersive, only one band would be expected because all the DNA would be the same weight. What appeared though was two bands. One was radioactive and the other was not. This el ...
Site-specific mutagenesis of M13 clones
Site-specific mutagenesis of M13 clones

... nut ( N protein utilization) site: (1) If no N protein, RNA polymerase will ignore the nut site and fall off the DNA, releasing the mRNA when it reaches the downstream stop signal. (2) In the presence of N protein, RNA polymerase will pass over nut and ignore the downstream stop signal. ...
Ch. 12 Introduction to Biotechnology
Ch. 12 Introduction to Biotechnology

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Telomeres - OpenWetWare
Telomeres - OpenWetWare

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Presentation 1 Guidelines

... nucleotide, the phosphate is already linked to the 5 position on the sugar. When two nucleotides are hooked together, a phosphate on one nucleotide forms a covalent bond with a hydroxyl group at the 3 position on another nucleotide. C7. The bases conform to the AT/GC rule of complementarity. There ...
Supplementary Information (doc 59K)
Supplementary Information (doc 59K)

... For identification of relevant promoter methylation we analyzed the colorectal cancer cell lines SW480 and Caco-2 using two Methyl-Profiler DNA Methylation PCR Array Systems (Qiagen, Hilden, Germany) which enables fast and accurate detection of DNA methylation status at CpG islands. The Human Colon ...
Gene Section ALOX5 (arachidonate 5-lipoxygenase) Atlas of Genetics and Cytogenetics
Gene Section ALOX5 (arachidonate 5-lipoxygenase) Atlas of Genetics and Cytogenetics

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Protein synthesis File
Protein synthesis File

... template; which strands are copied; the nucleotides involved and any differences in their composition, e.g. pentose sugar involved; complementary pairing; other features, e.g. nature of the resulting polynucleotide. ...
Exam Review 2 10/2/16
Exam Review 2 10/2/16

... A. The Y-shaped region where the DNA is split into two separate strands for coding B. Growing as DNA replication proceeds because synthesis is bidirectional C. The location at which the replication process begins D. Present only in bacterial cells and not in eukaryotes 45. Reverse transcriptase is c ...
Week 39 (2015-09-21)
Week 39 (2015-09-21)

... most satisfied with the process, biobank, and hypothetical IRRs received. The None group was least satisfied and least likely to agree that the biobank was beneficial (p < .001). The response to the statement that the biobank was harmful was not different between groups. Our data suggest that the ab ...
Quantitative RT-PCR
Quantitative RT-PCR

... The aim is to make a small internal deletion in the cDNA between the two primers used for PCR. The modified template will be used to make a control product which is shorter than the product obtained with wild type RNA. a. Clone the cDNA fragment containing the region between the 5' and 3' primers in ...
File - Ms. Poole`s Biology
File - Ms. Poole`s Biology

... • 1. Ribosomes-are made from proteins and rRNA. It has two subunits, a small subunit and a large subunit. There are three sites where the tRNA attaches. •The A site is where the tRNA arrives with the amino acid. •The P site has a tRNA that attaches to the tRNA at the A site. ...
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... Add DNA Polymerase. It will start adding bases using the unknown strand as a template. Every time a nucleotide with a dye is used the newly forming strand falls off the template strand. This means there will be many strands of varying length. Each of these pieces will have a different color dye. Pla ...
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Sequencing and Phylogeny - World Health Organization

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BIO S - Chapter 13 RNA

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RNA/Protein Purification 96-Well Kit
RNA/Protein Purification 96-Well Kit

... from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. It is often necessary to isolate total RNA and proteins from a single sample, such as for studies of gene expression including gene silencing experiments, mRNA knockdowns or experiments corr ...
Chapter 4 • Lesson 20
Chapter 4 • Lesson 20

... contain two types of nucleic acids—DNA (deoxyribonucleic acid) and RNA (ribonucleic acid). Each nucleic acid is named for the sugar it contains: deoxyribose in DNA and ribose in RNA. You will learn more about RNA in the next lesson. DNA and RNA both contain fivecarbon sugar molecules. Although the s ...
Technical Information and Test Overview
Technical Information and Test Overview

... identify all 4 types of genomic alterations across all genes known to be unambiguous drivers of solid tumors with high accuracy. The test simultaneously sequences the coding region of 315 cancer-related genes plus introns from 28 genes often rearranged or altered in cancer to a typical median depth ...
View PDF - CiteSeerX
View PDF - CiteSeerX

... [1,2]. A cis-acting promoter element, the GCC box, is both necessary and su⁄cient to confer ethylene responsiveness to a number of these PR genes in several plant species [3,4]. Ethylene response factors (ERFs), trans-acting factors that speci¢cally bind the GCC box, have been identi¢ed in several p ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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