![Market America Intranet](http://s1.studyres.com/store/data/007873409_1-8bba37962a069fc4623a1ce37acbe72d-300x300.png)
Isolation and amplification of ancient DNA
... basing on analyses of museum and non-invasively collected specimens – the amount of endogenous DNA available in the samples is often limited. Thus, techniques that provide extraction of the highest possible amount of DNA molecules from a specimen are of crucial importance. Consequently, most of extr ...
... basing on analyses of museum and non-invasively collected specimens – the amount of endogenous DNA available in the samples is often limited. Thus, techniques that provide extraction of the highest possible amount of DNA molecules from a specimen are of crucial importance. Consequently, most of extr ...
Packet #3
... 6. You have a plasmid with genes for tetracycline resistance and ampicillin resistance, as shown in the diagram 6a. In the middle of the tetracycline resistance gene is a target site for the restriction enzyme BamHI. Therefore, when you insert the gene of interest into this plasmid using the BAMHI ...
... 6. You have a plasmid with genes for tetracycline resistance and ampicillin resistance, as shown in the diagram 6a. In the middle of the tetracycline resistance gene is a target site for the restriction enzyme BamHI. Therefore, when you insert the gene of interest into this plasmid using the BAMHI ...
Schematic courtesy of B. Crump Quantitative (Real Time) PCR
... 6. Elute DNA off membrane with pH 8, low-salt buffer ...
... 6. Elute DNA off membrane with pH 8, low-salt buffer ...
PowerPoint
... Restriction enzymes cut plasmid and foreign DNA Foreign gene inserted into plasmid ...
... Restriction enzymes cut plasmid and foreign DNA Foreign gene inserted into plasmid ...
Electrophoretic Properties of Native Proteins
... groups. The precise three-dimensional configuration of a protein is critical to its biological function. The general shapes for proteins are spherical, elliptical or rod-like. The molecular weight is a function of the number and type of amino acids in the polypeptide chain. Proteins can consist of a ...
... groups. The precise three-dimensional configuration of a protein is critical to its biological function. The general shapes for proteins are spherical, elliptical or rod-like. The molecular weight is a function of the number and type of amino acids in the polypeptide chain. Proteins can consist of a ...
DNA and RNA
... DNA and RNA both carry genetic information, there are quite a few differences between them. This is a comparison of the differences between DNA versus RNA, including a quick summary and a detailed table of the differences. Summary of Differences between DNA and RNA ...
... DNA and RNA both carry genetic information, there are quite a few differences between them. This is a comparison of the differences between DNA versus RNA, including a quick summary and a detailed table of the differences. Summary of Differences between DNA and RNA ...
The DNA of microorganisms is made up of subunits called A
... The site where the old DNA strands separate and new DNA strands will be synthesized is called the A. primer. B. Okazaki fragment. C. template. D. rolling circle. E. replication fork. ...
... The site where the old DNA strands separate and new DNA strands will be synthesized is called the A. primer. B. Okazaki fragment. C. template. D. rolling circle. E. replication fork. ...
DNA
... Discontinuous synthesis produces 5’ to 3’ DNA segments called Okazaki fragments Exonuclease enzymes remove RNA primers. ...
... Discontinuous synthesis produces 5’ to 3’ DNA segments called Okazaki fragments Exonuclease enzymes remove RNA primers. ...
Discovery of a “transforming principle”
... Discontinuous synthesis produces 5’ to 3’ DNA segments called Okazaki fragments Exonuclease enzymes remove RNA primers. ...
... Discontinuous synthesis produces 5’ to 3’ DNA segments called Okazaki fragments Exonuclease enzymes remove RNA primers. ...
RESTRICTION ENDONUCLEASES
... This is done by the modification of the DNA at specific sites which prevents the restriction enzymes from binding or cleaving the DNA. Restriction enzymes are distinguished according to their recognition sequence and their cleavage ...
... This is done by the modification of the DNA at specific sites which prevents the restriction enzymes from binding or cleaving the DNA. Restriction enzymes are distinguished according to their recognition sequence and their cleavage ...
DNA
... chromosome is circular and not linear like eukaryotic cells. There is also only one origin for replication which attached to the plasma membrane. Replication of the chromosome occurs in both directions like eukaryotes. Prokaryotes have far fewer DNA base pairs than eukaryotes. E. coli has about 4.6 ...
... chromosome is circular and not linear like eukaryotic cells. There is also only one origin for replication which attached to the plasma membrane. Replication of the chromosome occurs in both directions like eukaryotes. Prokaryotes have far fewer DNA base pairs than eukaryotes. E. coli has about 4.6 ...
Human DNA Dance - University of Wisconsin Biotechnology Center
... However, the Human DNA Model is among the easiest ways to show the idea of "antiparallel" because the two lines of people face opposite directions as they shake hands, like two teams after a baseball game. Few models of DNA actually show the antiparallel nature of DNA, and of the models that do show ...
... However, the Human DNA Model is among the easiest ways to show the idea of "antiparallel" because the two lines of people face opposite directions as they shake hands, like two teams after a baseball game. Few models of DNA actually show the antiparallel nature of DNA, and of the models that do show ...
For the 5 W`s Flipbook you need to complete tRNA and rRNA (this is
... tRNA brings the corresponding amino acid based off of its anticodon recognizing mRNA’s codon. 10. What is this stage called, when mRNA gives the message to tRNA? Translation 11. If you want to know the name of the amino acid that a gene gives the instructions for, what trick can you use? (Without ev ...
... tRNA brings the corresponding amino acid based off of its anticodon recognizing mRNA’s codon. 10. What is this stage called, when mRNA gives the message to tRNA? Translation 11. If you want to know the name of the amino acid that a gene gives the instructions for, what trick can you use? (Without ev ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.