SBI4U: DNA Replication - SBI4U with Ms. Taman!
... Two parental strands separate and each serves as as a _____________ for a new ________________ Newly synthesized DNA molecule has __________ _________________________________________ 2. Conservative The two parental strands _____________________ and produce another daughter helix with ________ ...
... Two parental strands separate and each serves as as a _____________ for a new ________________ Newly synthesized DNA molecule has __________ _________________________________________ 2. Conservative The two parental strands _____________________ and produce another daughter helix with ________ ...
Miscellaneous Bioseparation
... complex mixtures One way to increase the drift speed is to increase the electric field strength However, there are limits to this strategy because very large electric fields can heat the large surfaces of an electrophoresis apparatus unevenly, leading to nonuniform distribution of electrophoretic mo ...
... complex mixtures One way to increase the drift speed is to increase the electric field strength However, there are limits to this strategy because very large electric fields can heat the large surfaces of an electrophoresis apparatus unevenly, leading to nonuniform distribution of electrophoretic mo ...
Handout
... This loss of charge causes them to move very slowly in the electric field. The Cl- ions (from Tris-HCl) on the other hand, move much more quickly in the electric field and they form an ion front that migrates ahead of the glycine. The separation of Cl- from the Tris counter-ion (which is now moving ...
... This loss of charge causes them to move very slowly in the electric field. The Cl- ions (from Tris-HCl) on the other hand, move much more quickly in the electric field and they form an ion front that migrates ahead of the glycine. The separation of Cl- from the Tris counter-ion (which is now moving ...
Grading rubric DNA Project Unit
... Requirements: complete sentences, word processed, correct 2. Project DNA chart Requirements: shows 6 codons for DNA and RNA, 6 amino acids, 6 traits 3. DNA transcribed into RNA Requirements: all DNA translated correctly into RNA 6 traits should be visible 4. Colored picture of the person Requirement ...
... Requirements: complete sentences, word processed, correct 2. Project DNA chart Requirements: shows 6 codons for DNA and RNA, 6 amino acids, 6 traits 3. DNA transcribed into RNA Requirements: all DNA translated correctly into RNA 6 traits should be visible 4. Colored picture of the person Requirement ...
Honors_Genetics_B_Student_Notes
... Restriction Fragment Length Polymorphisms (RFLP): differences in DNA fragments sizes cut by restriction enzymes RFLP analysis: - restriction enzyme cuts everyone's DNA at different places - creates fragments with different lengths ...
... Restriction Fragment Length Polymorphisms (RFLP): differences in DNA fragments sizes cut by restriction enzymes RFLP analysis: - restriction enzyme cuts everyone's DNA at different places - creates fragments with different lengths ...
Modeling DNA Structure and Function
... III. Transcription Using the DNA molecule that you've just created, do the following: Build an mRNA molecule that is complementary to one of the DNA strands -- the so called template strand. That is, show your instructor what would happen if the DNA was being transcribed. ...
... III. Transcription Using the DNA molecule that you've just created, do the following: Build an mRNA molecule that is complementary to one of the DNA strands -- the so called template strand. That is, show your instructor what would happen if the DNA was being transcribed. ...
Document
... 1. isolation of foreign DNA to be inserted 2. joining of DNA to vector 3. actual transfer of DNA to host D. DNA can only be recombined if isolated & snipped into smaller fragments Restriction Enzymes - bacterial proteins (100's) that cut DNA strands - cuts at specific point in nucleotide sequence - ...
... 1. isolation of foreign DNA to be inserted 2. joining of DNA to vector 3. actual transfer of DNA to host D. DNA can only be recombined if isolated & snipped into smaller fragments Restriction Enzymes - bacterial proteins (100's) that cut DNA strands - cuts at specific point in nucleotide sequence - ...
5 questions per round and 9 rounds with 10 team tourney
... 49. What are the three differences between RNA and DNA? (different sugar, U instead of T, and single stranded instead of double stranded) 50. What was radioactively labeled by Hershey and Chase to find out if DNA was the critical molecule? (bacteriophage) 51. What is a mutated gene that leads a cell ...
... 49. What are the three differences between RNA and DNA? (different sugar, U instead of T, and single stranded instead of double stranded) 50. What was radioactively labeled by Hershey and Chase to find out if DNA was the critical molecule? (bacteriophage) 51. What is a mutated gene that leads a cell ...
Document
... DNA is purified from the sample, in the example a blood stain, the very long DNA molecules are cut with a restriction enzyme (these are very important tools for geneticists, they cut the DNA precisely, only at certain short DNA sequences producing reproducible patterns of fragments). This step pro ...
... DNA is purified from the sample, in the example a blood stain, the very long DNA molecules are cut with a restriction enzyme (these are very important tools for geneticists, they cut the DNA precisely, only at certain short DNA sequences producing reproducible patterns of fragments). This step pro ...
notes_14C_nucacids
... - Plain pyrimidines and purines have low solubility (not many polar bonds) ...
... - Plain pyrimidines and purines have low solubility (not many polar bonds) ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.