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- fiveless|notes
... SDS-PAGE (Sodium dodecyl sulphate polyacrylamide gel electrophoresis) is used for analysis of proteins ...
... SDS-PAGE (Sodium dodecyl sulphate polyacrylamide gel electrophoresis) is used for analysis of proteins ...
Copying DNA: Southern Blotting
... P C R I S A R A P I D M E T H O D F O R C LO N I N G G E N E S ...
... P C R I S A R A P I D M E T H O D F O R C LO N I N G G E N E S ...
Genetic engineering
... • A restriction enzyme: enzyme that cuts the DNA at a specific code. • There are thousands of restriction enzymes. • Each cuts DNA at a different sequence. • Some look for GGCC and cut in between the G and C. • Every time GGCC is found in the DNA it is cut by the restriction enzyme ...
... • A restriction enzyme: enzyme that cuts the DNA at a specific code. • There are thousands of restriction enzymes. • Each cuts DNA at a different sequence. • Some look for GGCC and cut in between the G and C. • Every time GGCC is found in the DNA it is cut by the restriction enzyme ...
Building a DNA molecule
... Insulin, like many proteins, is made up of two long chains of amino acids. Each protein chain is coded by genes located on the DNA helix in the nuclei of your cells. Each pair of students in the class will be assigned one of these amino acids in the chain. Directions: You will be assigned an amino a ...
... Insulin, like many proteins, is made up of two long chains of amino acids. Each protein chain is coded by genes located on the DNA helix in the nuclei of your cells. Each pair of students in the class will be assigned one of these amino acids in the chain. Directions: You will be assigned an amino a ...
Cloning in Escherichia coli
... products of this ligation reaction into E. coli cells in a standard technique called a transformation experiment, and select for cells that have taken up plasmid DNA, based on an ampicillin resistant phenotype conferred to the cells by a plasmid-encoded gene (Ampr – see plasmid image on next page). ...
... products of this ligation reaction into E. coli cells in a standard technique called a transformation experiment, and select for cells that have taken up plasmid DNA, based on an ampicillin resistant phenotype conferred to the cells by a plasmid-encoded gene (Ampr – see plasmid image on next page). ...
Plasmids, primers (and beyond!)
... Cohesive ends can be formed on a DNA fragment (e.g., a synthetic oligonucleotide) by adding a short segment using a DNA ligase (such as the one from T4 ligase). The short segment contains a cleavage site for a restriction enzyme, in this case, EcoR1. EcoR1 forms an overhanging adhesive site. ...
... Cohesive ends can be formed on a DNA fragment (e.g., a synthetic oligonucleotide) by adding a short segment using a DNA ligase (such as the one from T4 ligase). The short segment contains a cleavage site for a restriction enzyme, in this case, EcoR1. EcoR1 forms an overhanging adhesive site. ...
In 1953 Watson and Crick developed a double helix model for DNA
... The two nucleic acid strands are _______________________ to each other. That means that one strand is “_____________________” compared to the other. The 2 nucleic acid strands are held together by ____________ _ _____________ between the nitrogen bases. When the nitrogen bases bond together they fol ...
... The two nucleic acid strands are _______________________ to each other. That means that one strand is “_____________________” compared to the other. The 2 nucleic acid strands are held together by ____________ _ _____________ between the nitrogen bases. When the nitrogen bases bond together they fol ...
1 - contentextra
... The elements carbon, hydrogen, oxygen and nitrogen are the most frequently occurring in living organisms although life would not be possible without small amounts of elements such as sulfur, calcium, phosphorus, iron and sodium. ...
... The elements carbon, hydrogen, oxygen and nitrogen are the most frequently occurring in living organisms although life would not be possible without small amounts of elements such as sulfur, calcium, phosphorus, iron and sodium. ...
1 - contentextra
... The elements carbon, hydrogen, oxygen and nitrogen are the most frequently occurring in living organisms although life would not be possible without small amounts of elements such as sulfur, calcium, phosphorus, iron and sodium. ...
... The elements carbon, hydrogen, oxygen and nitrogen are the most frequently occurring in living organisms although life would not be possible without small amounts of elements such as sulfur, calcium, phosphorus, iron and sodium. ...
Nucleic Acids - New Jersey Institute of Technology
... transformation by drawing a colorful diagram to illustrate this experiment and writing an explanation of this diagram. Summarize how Avery’s experiments resulted in the conclusion that DNA is responsible for transformation in bacteria by after reading the Avery’s Experiment hand out. Compare and ...
... transformation by drawing a colorful diagram to illustrate this experiment and writing an explanation of this diagram. Summarize how Avery’s experiments resulted in the conclusion that DNA is responsible for transformation in bacteria by after reading the Avery’s Experiment hand out. Compare and ...
The Structure of DNA
... • Maurice Wilkins and Rosalind Franklin used Xray crystallography to study the structure of DNA. – In this technique, X-rays are diffracted as they passed through aligned fibers of purified DNA. – The diffraction pattern can be used to deduce the three-dimensional shape of molecules. • James Watson ...
... • Maurice Wilkins and Rosalind Franklin used Xray crystallography to study the structure of DNA. – In this technique, X-rays are diffracted as they passed through aligned fibers of purified DNA. – The diffraction pattern can be used to deduce the three-dimensional shape of molecules. • James Watson ...
FEBS Letters
... IN inhibition assays for wild type (WT) and each mutant followed the same general procedure. To determine the degree of inhibition on both 3’-processing and strand transfer, purified IN enzyme (final concentration: 200 nM) was pre-incubated with peptide (dissolved in water; stored at -20° C) in a re ...
... IN inhibition assays for wild type (WT) and each mutant followed the same general procedure. To determine the degree of inhibition on both 3’-processing and strand transfer, purified IN enzyme (final concentration: 200 nM) was pre-incubated with peptide (dissolved in water; stored at -20° C) in a re ...
Reagents - NutriTech
... TRI Reagent is a complete and ready-to-use reagent for isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. The technique performs well with small and large quantities of tissue or cultured cells, and a ...
... TRI Reagent is a complete and ready-to-use reagent for isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. The technique performs well with small and large quantities of tissue or cultured cells, and a ...
Biotechnology IB Syllabus
... International-mindedness: Gel electrophoresis is used to separate proteins or fragments of DNA according to size. PCR can be used to amplify small amounts of DNA. Theory of knowledge: DNA profiling involves comparison of DNA. The use of DNA for securing convictions in legal cases is well Gen ...
... International-mindedness: Gel electrophoresis is used to separate proteins or fragments of DNA according to size. PCR can be used to amplify small amounts of DNA. Theory of knowledge: DNA profiling involves comparison of DNA. The use of DNA for securing convictions in legal cases is well Gen ...
Determination of the pH Scale by the Method of
... where Hdyea+DNAa means a single molecule consisting of Hdyea+ bound to DNAa. Your company is building an apparatus that can tell whether water is contaminated with the viral DNA (DNAa). To calibrate the instrument, your co-workers would like a solution in which [HDyea+DNAa]/[DNAa] = 1. Due to the ...
... where Hdyea+DNAa means a single molecule consisting of Hdyea+ bound to DNAa. Your company is building an apparatus that can tell whether water is contaminated with the viral DNA (DNAa). To calibrate the instrument, your co-workers would like a solution in which [HDyea+DNAa]/[DNAa] = 1. Due to the ...
bioknowledgy note pkt - Peoria Public Schools
... 2.6.U3 DNA is a double helix made of two antiparallel strands of nucleotides linked by hydrogen bonding between complementary base pairs. (includes 2.6.S1 Drawing simple diagrams of the structure of single nucleotides of DNA and RNA, using circles, pentagons and rectangles to represent phosphates, p ...
... 2.6.U3 DNA is a double helix made of two antiparallel strands of nucleotides linked by hydrogen bonding between complementary base pairs. (includes 2.6.S1 Drawing simple diagrams of the structure of single nucleotides of DNA and RNA, using circles, pentagons and rectangles to represent phosphates, p ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.