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DNA, RNA and Protein Power Point
... phosphates form the sides of a ladder and the nitrogen bases form the rungs. The Two sides of the ladder are held together with hydrogen bonds ...
... phosphates form the sides of a ladder and the nitrogen bases form the rungs. The Two sides of the ladder are held together with hydrogen bonds ...
DNA REPLICATION Review of DNA Structure
... • Leading strand – elongates toward the replication fork, continuous • Lagging strand – elongates away from the replication fork – Okazaki fragments: discontinuous short segments ...
... • Leading strand – elongates toward the replication fork, continuous • Lagging strand – elongates away from the replication fork – Okazaki fragments: discontinuous short segments ...
Additional Slides Ch Biotech Dr Violet
... variable number of tandem repeats. These are short sequences of DNA at scattered locations in the genome, repeated in tandem (like freight cars of a train). • The number of these repeat units varies from person to person, but is unique for any given individual and, therefore, serves as a molecular f ...
... variable number of tandem repeats. These are short sequences of DNA at scattered locations in the genome, repeated in tandem (like freight cars of a train). • The number of these repeat units varies from person to person, but is unique for any given individual and, therefore, serves as a molecular f ...
Chapter 12 Learning Objectives
... for a single amino acid and entire protein chains of amino acids) 14. Explain the differences between the three types of RNA and explain their roles 15. Explain that changing the activity of proteins within cells and/or by changing whether and how often particular genes are expressed (i.e. “regulati ...
... for a single amino acid and entire protein chains of amino acids) 14. Explain the differences between the three types of RNA and explain their roles 15. Explain that changing the activity of proteins within cells and/or by changing whether and how often particular genes are expressed (i.e. “regulati ...
DNA Review (study guide)
... 2. In a single strand of DNA, the phosphate group binds to the __________________ of the next group. 3. Base pairing rule states that the DNA of any species contains equal amounts of __________________ & ____________ and also equal amounts of __________________ & ____________________ 4. Wilkins and ...
... 2. In a single strand of DNA, the phosphate group binds to the __________________ of the next group. 3. Base pairing rule states that the DNA of any species contains equal amounts of __________________ & ____________ and also equal amounts of __________________ & ____________________ 4. Wilkins and ...
Frontiers of Biotechnology
... – The molecules will move from __________________________________________ Manipulating DNA ...
... – The molecules will move from __________________________________________ Manipulating DNA ...
Isolating, Cloning and Sequencing DNA
... proportions of the final product, especially if the error occurred early on during PCR ...
... proportions of the final product, especially if the error occurred early on during PCR ...
UltraClean 15 DNA Purification Kit
... silica is kept in suspension and not allowed to settle during the binding step. Recommended Gel Running Conditions We recommend pH 7.5 - 7.8 for running all TAE gels and pH 8.0 - 8.3 for TBE gels. Agarose gel concentrations up to 4% are compatible with this kit. All agarose brands and types can be u ...
... silica is kept in suspension and not allowed to settle during the binding step. Recommended Gel Running Conditions We recommend pH 7.5 - 7.8 for running all TAE gels and pH 8.0 - 8.3 for TBE gels. Agarose gel concentrations up to 4% are compatible with this kit. All agarose brands and types can be u ...
Bacterial transformation - BLI-Research-Synbio-2014-session-1
... over blunt end cutters because DNA fragments can be joined easily together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) ...
... over blunt end cutters because DNA fragments can be joined easily together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) ...
Biotechnology 2
... Many uses of restriction enzymes… Now that we can cut DNA with restriction enzymes… we can cut up DNA from different people… or different organisms… and compare it why? ...
... Many uses of restriction enzymes… Now that we can cut DNA with restriction enzymes… we can cut up DNA from different people… or different organisms… and compare it why? ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.