![Capillary Electrophoresis of Oligonucleotides](http://s1.studyres.com/store/data/021929951_1-4de8a1a813ef93bd074757dff06d401b-300x300.png)
Exam 2
... Electrophoresis is used to separate DNA. Fragments migrate according to size, with larger fragments migrating more slowly than smaller fragments. ...
... Electrophoresis is used to separate DNA. Fragments migrate according to size, with larger fragments migrating more slowly than smaller fragments. ...
Manipulating and Analyzing DNA
... recombinant DNA. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? How and why are they used in biotechnology? How do restriction enzymes play a role in recombinant DNA? Restriction Enzym ...
... recombinant DNA. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? How and why are they used in biotechnology? How do restriction enzymes play a role in recombinant DNA? Restriction Enzym ...
Lab 1 Introduction to nucleic acids Structural Properties
... The sequence of one strand specifies the sequence of the other. ...
... The sequence of one strand specifies the sequence of the other. ...
Chapter 14: DNA Technologies
... G. A great deal of information can be inferred from a DNA nucleotide sequence from the chain termination method of DNA sequencing 1. This method was developed by Sanger and Gilbert in 1974, for which they received the Nobel Prize in 1980 2. Incorporation of dideoxynucleotides allows the investigator ...
... G. A great deal of information can be inferred from a DNA nucleotide sequence from the chain termination method of DNA sequencing 1. This method was developed by Sanger and Gilbert in 1974, for which they received the Nobel Prize in 1980 2. Incorporation of dideoxynucleotides allows the investigator ...
LEGO Activities
... This activity can be as “fancy” or as simple as you are willing to make it. Students need to work both within their group, and collaborate with other groups, to create a gel using their LEGO® stacks. (If groups are very large, students can choose a spokesperson to run the gel, and give advice and su ...
... This activity can be as “fancy” or as simple as you are willing to make it. Students need to work both within their group, and collaborate with other groups, to create a gel using their LEGO® stacks. (If groups are very large, students can choose a spokesperson to run the gel, and give advice and su ...
File
... What is Polymerase chain reaction? (PCR) PCR is a technique that is used to amplify one sample of DNA thousands of times over to create a large enough DNA sample for extensive analysis. ...
... What is Polymerase chain reaction? (PCR) PCR is a technique that is used to amplify one sample of DNA thousands of times over to create a large enough DNA sample for extensive analysis. ...
Recombinant DNA and Genetic Engineering
... • Sequence to be copied is heated • Primers are added and bind to ends of single strands • DNA polymerase uses free nucleotides to create complementary strands • Doubles number of copies of DNA ...
... • Sequence to be copied is heated • Primers are added and bind to ends of single strands • DNA polymerase uses free nucleotides to create complementary strands • Doubles number of copies of DNA ...
Make a DNA Model - Flinn Scientific
... in DNA—adenine, cytosine, thymine, and guanine. The bases on opposite strands in the double-stranded structure of DNA are complementary, meaning that adenine only pairs with thymine and cytosine only pairs with guanine in DNA. This specific pairing assists in creating the famous helical structure of ...
... in DNA—adenine, cytosine, thymine, and guanine. The bases on opposite strands in the double-stranded structure of DNA are complementary, meaning that adenine only pairs with thymine and cytosine only pairs with guanine in DNA. This specific pairing assists in creating the famous helical structure of ...
Mr Proffitt – IB Biology Name Unit 3 Test Multiple Choice – 1 Mark
... Short lengths of RNA primase attached to the DNA during replication B. Short sections of DNA formed during DNA replication C. Nucleotides added by DNA polymerase I in the same direction as the replication fork D. Sections of RNA removed by DNA polymerase III and replaced with DNA ...
... Short lengths of RNA primase attached to the DNA during replication B. Short sections of DNA formed during DNA replication C. Nucleotides added by DNA polymerase I in the same direction as the replication fork D. Sections of RNA removed by DNA polymerase III and replaced with DNA ...
The Structure of DNA and RNA
... Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way in which letters of the alphabet appear in a certa ...
... Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way in which letters of the alphabet appear in a certa ...
DNA Libraries
... Gel Electrophoresis …electrophoresis is the movement of charged particles in an electric field, …DNA with it’s phosphate backbone carries a net negative charge, – DNA migrates toward the positive pole in an electrical field. ...
... Gel Electrophoresis …electrophoresis is the movement of charged particles in an electric field, …DNA with it’s phosphate backbone carries a net negative charge, – DNA migrates toward the positive pole in an electrical field. ...
Department of Chemistry IIT Kharagpur Biochemical Techniques
... Ion exchange chromatography is a separation technique used for purification or analysis of molecules based their charge. The method can be used to separate charged molecules from uncharged ones or it can separate molecules of different charge from one another. Principle of the method: Ionizable chem ...
... Ion exchange chromatography is a separation technique used for purification or analysis of molecules based their charge. The method can be used to separate charged molecules from uncharged ones or it can separate molecules of different charge from one another. Principle of the method: Ionizable chem ...
Classwork May 15th
... 10. Each individual DNA strand serves as a __________________ or model for the formation of other DNA molecules by replication. [1pt] Gene-Chromosome Model (chapter 20) 11. Use the following diagram to describe the relationship between genes, DNA, and chromosomes. [3pts] ...
... 10. Each individual DNA strand serves as a __________________ or model for the formation of other DNA molecules by replication. [1pt] Gene-Chromosome Model (chapter 20) 11. Use the following diagram to describe the relationship between genes, DNA, and chromosomes. [3pts] ...
Replication/Transcription/Translation
... 1. Name the 3 essential enzymes for replication. DNA helicase, polymerase, and ligase 2. Describe the semi-conservative model. The parent strand acts at the model for the new daughter ...
... 1. Name the 3 essential enzymes for replication. DNA helicase, polymerase, and ligase 2. Describe the semi-conservative model. The parent strand acts at the model for the new daughter ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.