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Biotechnology
... Recognize and cut at specific places along the DNA molecule called restriction sites. Each different restriction enzyme has its own type of site. Restriction site is a 4 or 6 base pair sequence that is a palindrome. A DNA palidrome is a sequence in which the “top strand read from left to right is th ...
... Recognize and cut at specific places along the DNA molecule called restriction sites. Each different restriction enzyme has its own type of site. Restriction site is a 4 or 6 base pair sequence that is a palindrome. A DNA palidrome is a sequence in which the “top strand read from left to right is th ...
DNA Technology - University of Evansville Faculty Web sites
... The enzyme cuts the plasmid DNA at one specific restriction site; it also cuts the human DNA 3. The human DNA is mixed with the cut plasmid 4. The enzyme DNA ligase joins the 2 DNA molecules by covalent bonds 5. The recombinant plasmid is added to a bacterium 6. The production of multiple copies of ...
... The enzyme cuts the plasmid DNA at one specific restriction site; it also cuts the human DNA 3. The human DNA is mixed with the cut plasmid 4. The enzyme DNA ligase joins the 2 DNA molecules by covalent bonds 5. The recombinant plasmid is added to a bacterium 6. The production of multiple copies of ...
Assignment DNA - UniMAP Portal
... 6. The polymerase chain reaction (PCR) is a technique to produce a large number of identical molecules of DNA in vitro. Describe in detail the PCR technique. Principles of PCR: Denaturation. Exposure to heat (about 94°C) separates the two strands of the target DNA by breaking the hydrogen bon ...
... 6. The polymerase chain reaction (PCR) is a technique to produce a large number of identical molecules of DNA in vitro. Describe in detail the PCR technique. Principles of PCR: Denaturation. Exposure to heat (about 94°C) separates the two strands of the target DNA by breaking the hydrogen bon ...
Structure and Properties of DNA and Genes
... Wherever DNA is found, its basic structure is the same. DNA is formed as a double-stranded molecule called a double helix. Essentially, a double helix is like a ladder that has been twisted around. The ‘legs’ of the DNA double helix are made up of a sugar-phosphate backbone. These backbones consist ...
... Wherever DNA is found, its basic structure is the same. DNA is formed as a double-stranded molecule called a double helix. Essentially, a double helix is like a ladder that has been twisted around. The ‘legs’ of the DNA double helix are made up of a sugar-phosphate backbone. These backbones consist ...
Lab - Recombinant DNA Simulation
... one cut between the G and A in each of the DNA strands (see below). After the cuts are made, the DNA is held together only by weak hydrogen bonds between the four bases AATT. These bonds are easily broken apart. ...
... one cut between the G and A in each of the DNA strands (see below). After the cuts are made, the DNA is held together only by weak hydrogen bonds between the four bases AATT. These bonds are easily broken apart. ...
2. You perform a Southern blot in which your probe should hybridize
... #2. You perform a Southern blot in which your probe should hybridize to a single DNA band. Blot I : Name THREE possible problems that could cause this (blank blot, no bands). 1. Failure of DNA to transfer to membrane 2. Forgot to bake membrane & DNA washed off 3. Didn’t digest enough DNA to detect 4 ...
... #2. You perform a Southern blot in which your probe should hybridize to a single DNA band. Blot I : Name THREE possible problems that could cause this (blank blot, no bands). 1. Failure of DNA to transfer to membrane 2. Forgot to bake membrane & DNA washed off 3. Didn’t digest enough DNA to detect 4 ...
Analysis of Gene Sequences
... Consider a segment of DNA that is about 1000 base pairs long that we wish to sequence. (1) The two DNA strands are separated. Heating to 100˚C to melt the base pairinghydrogen bonds that hold the strands together does this. (2) A short oligonucleotide (ca. 18 bases) designed to be complimentary to t ...
... Consider a segment of DNA that is about 1000 base pairs long that we wish to sequence. (1) The two DNA strands are separated. Heating to 100˚C to melt the base pairinghydrogen bonds that hold the strands together does this. (2) A short oligonucleotide (ca. 18 bases) designed to be complimentary to t ...
Agarose gel electrophoresis
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Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.