Topic 1: Cell biology (15 hours)
... 10. Application: Use of Taq DNA polymerase to produce depends on complementary base pairing. multiple copies of DNA rapidly by the polymerase 3. Helicase unwinds the double helix and separates the chain reaction (PCR). two strands by breaking hydrogen bonds. 11. Application: Production of human insu ...
... 10. Application: Use of Taq DNA polymerase to produce depends on complementary base pairing. multiple copies of DNA rapidly by the polymerase 3. Helicase unwinds the double helix and separates the chain reaction (PCR). two strands by breaking hydrogen bonds. 11. Application: Production of human insu ...
Restriction Enzymes
... DNA Cloning, II • Bacterial plasmids (small circular DNA additional to a bacteria’s regular DNA) are cut with the same restriction enzyme • A chunk of DNA can thus be inserted into the plasmid DNA to form a “recombinant” ...
... DNA Cloning, II • Bacterial plasmids (small circular DNA additional to a bacteria’s regular DNA) are cut with the same restriction enzyme • A chunk of DNA can thus be inserted into the plasmid DNA to form a “recombinant” ...
DNA
... This separation is maintained by a group of proteins includes: 1- Single stranded DNA-binding (SSB) proteins, also called: helixdestabilizing proteins: these bind to only single stranded DNA and keep two strands separated and prevent reformation of double helix. 2- DNA helicase: binds to single str ...
... This separation is maintained by a group of proteins includes: 1- Single stranded DNA-binding (SSB) proteins, also called: helixdestabilizing proteins: these bind to only single stranded DNA and keep two strands separated and prevent reformation of double helix. 2- DNA helicase: binds to single str ...
Measuring forces in the DNA molecule
... To put it simply, the measurement system is designed hierarchically and involves microscopic beams, at the tips of which one or more double helix structures running in parallel are located. These have been modified such that each end carries one base pair. Two of these microscopic beams are connecte ...
... To put it simply, the measurement system is designed hierarchically and involves microscopic beams, at the tips of which one or more double helix structures running in parallel are located. These have been modified such that each end carries one base pair. Two of these microscopic beams are connecte ...
Chapter 20 DNA Metabolism Gene: A segment of DNA or RNA that
... For e.g. DNA Pol cannot seal nicks in DNA strands. Replisome: A complex of proteins involved in DNA replication. It consists of: Helicases: Use ATP to dissociate DNA strands. Topoisomerases: Relieve topological stress due to strand separation. ...
... For e.g. DNA Pol cannot seal nicks in DNA strands. Replisome: A complex of proteins involved in DNA replication. It consists of: Helicases: Use ATP to dissociate DNA strands. Topoisomerases: Relieve topological stress due to strand separation. ...
Untitled
... Large samples of plants and fungi. It contains a PVP solution to remove inhibitors. Toxic reagents are not used, the method can be scaled. Rapid and inexpensive. ...
... Large samples of plants and fungi. It contains a PVP solution to remove inhibitors. Toxic reagents are not used, the method can be scaled. Rapid and inexpensive. ...
Notes - The University of Sydney
... Nucleases: enzymes responsible for the hydrolysis of nucleic acids Exonucleases “chew” from the ends of the nucleic acid. Endonucleases eg. Restriction enzymes, cleave internal phosphodiester bonds and are usually sequence specific. There are 3 types of restriction enzymes; types I, II and III. Thes ...
... Nucleases: enzymes responsible for the hydrolysis of nucleic acids Exonucleases “chew” from the ends of the nucleic acid. Endonucleases eg. Restriction enzymes, cleave internal phosphodiester bonds and are usually sequence specific. There are 3 types of restriction enzymes; types I, II and III. Thes ...
TOPICS FOR EXAMINATION II – Biology 1406
... What is biotechnology?. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? ...
... What is biotechnology?. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? ...
DNA
... DNA Replication – Duplicating DNA • Before a cell divides, it duplicates its DNA in a process called replication. • Replication ensures that each resulting cell will have a complete set of DNA. • Occurs in the S phase of Interphase. ...
... DNA Replication – Duplicating DNA • Before a cell divides, it duplicates its DNA in a process called replication. • Replication ensures that each resulting cell will have a complete set of DNA. • Occurs in the S phase of Interphase. ...
Lab 11- DNA Structure and Function
... 2. Sprinkle in 2 g of wheat germ into the beaker and gently stir in 3 mL of detergent. Incubate this mixture in the warm water bath for 5 minutes. A. Detergents dissolve lipids and proteins that form the cell membranes found in the wheat germ by disrupting the chemical bonds that hold the membrane ...
... 2. Sprinkle in 2 g of wheat germ into the beaker and gently stir in 3 mL of detergent. Incubate this mixture in the warm water bath for 5 minutes. A. Detergents dissolve lipids and proteins that form the cell membranes found in the wheat germ by disrupting the chemical bonds that hold the membrane ...
DNA
... on a DNA strand. Possibilities become greater when one deals with two ch. Each containing different lengths of repeat sequ. ...
... on a DNA strand. Possibilities become greater when one deals with two ch. Each containing different lengths of repeat sequ. ...
DNA SEQUENCING DNA sequencing
... This method is based on the readout of electrical signal occurring at nucleotides passing by alpha-hemolysin pores covalently bound with cyclodextrin. The DNA passing through the nanopore changes its ion current. This change is dependent on the shape, size and length of the DNA sequence. Each type o ...
... This method is based on the readout of electrical signal occurring at nucleotides passing by alpha-hemolysin pores covalently bound with cyclodextrin. The DNA passing through the nanopore changes its ion current. This change is dependent on the shape, size and length of the DNA sequence. Each type o ...
Final Examination
... 8) The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pKaCOOH = 2.0; pKaNH2 = 10.6), and the mixture is spotted on a piece of filter paper and placed in an electric field at pH 6.15. Which statement is true? A) Both amino acids will move from the spot and be separate ...
... 8) The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pKaCOOH = 2.0; pKaNH2 = 10.6), and the mixture is spotted on a piece of filter paper and placed in an electric field at pH 6.15. Which statement is true? A) Both amino acids will move from the spot and be separate ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.