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Bio 313 worksheet 2 - Iowa State University
... technique called electrophoresis. With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole? ...
... technique called electrophoresis. With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole? ...
Chapter 13 Genetic Engineering Changing the living world
... The foreign DNA is first joined to a small, circular DNA known as a plasmid. Plasmids are found naturally in some bacteria and have been very useful for DNA transfer. Why? The plasmid has a genetic “marker”... a gene to distinguish which bacteria carry the foreign DNA. How? ...
... The foreign DNA is first joined to a small, circular DNA known as a plasmid. Plasmids are found naturally in some bacteria and have been very useful for DNA transfer. Why? The plasmid has a genetic “marker”... a gene to distinguish which bacteria carry the foreign DNA. How? ...
Laboratory 11
... order to visualize the DNA, and to measure its size, we will be carrying out a simple gel electrophoresis. Electrophoresis separates DNA according to its size by drawing it through an agarose gel using an electric field. DNA is negatively charged so it is attracted to the positive electrode in the c ...
... order to visualize the DNA, and to measure its size, we will be carrying out a simple gel electrophoresis. Electrophoresis separates DNA according to its size by drawing it through an agarose gel using an electric field. DNA is negatively charged so it is attracted to the positive electrode in the c ...
Lab23
... Add dye to PCR samples and load as much of each sample as will fit in the well into the good experimental gel: no air bubbles in tip while loading! Be certain power source is set to 70 VOLTS not AMPS Run gels for ~1.5 hrs (have lecture while waiting) Stain and destain gel Interpret results ...
... Add dye to PCR samples and load as much of each sample as will fit in the well into the good experimental gel: no air bubbles in tip while loading! Be certain power source is set to 70 VOLTS not AMPS Run gels for ~1.5 hrs (have lecture while waiting) Stain and destain gel Interpret results ...
Bio07_TR__U04_CH13.QXD
... 2. Crossing dissimilar individuals to bring together the best of both Organisms is called ________________________ . 3. The continued breeding of individuals with similar characteristics is called _______________________ . 4. Biologists change the DNA code of a living organism through ______________ ...
... 2. Crossing dissimilar individuals to bring together the best of both Organisms is called ________________________ . 3. The continued breeding of individuals with similar characteristics is called _______________________ . 4. Biologists change the DNA code of a living organism through ______________ ...
Gel Electrophoresis
... Ethidium bromide: It is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) mixed with the gel during its preparation. It is commonly ...
... Ethidium bromide: It is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) mixed with the gel during its preparation. It is commonly ...
Plasmid
... enzymes at the same time. You will need to determine the best buffer that works for both of your enzymes- BamHI and HindIII, by reading the instructions for your enzymes (in Fermentas catalog). ...
... enzymes at the same time. You will need to determine the best buffer that works for both of your enzymes- BamHI and HindIII, by reading the instructions for your enzymes (in Fermentas catalog). ...
13-2 Manipulating DNA
... have developed a series of tools that allow them to extract, edit, and then reinsert DNA into living organisms. ...
... have developed a series of tools that allow them to extract, edit, and then reinsert DNA into living organisms. ...
Introduction to gel electrophoresis
... 25Kb DNA fragments. • DNA has negatively charged phosphates along the DNA backbone. ...
... 25Kb DNA fragments. • DNA has negatively charged phosphates along the DNA backbone. ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.