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Shotgun sequencing
Shotgun sequencing

... Sanger DNA sequencing, as you may guess, only works for a certain distance beyond the sequencing primer (best from about 30 nt to 350 nt; the “read length”). Beyond that, very few products are produced because chain termination has already occurred. Therefore, to sequence a longer DNA, special metho ...
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For SNP microarray analysis processed before Oct. 15, 2012
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... Current Methodology: As of October 1, 2010 METHODOLOGY: The microarray analysis was performed using the Infinium Assay with the Illumina HD HumanOmni1-quad BeadChip platform. This chip contains approximately 1,140,419 probes including both single nucleotide polymorphisms (SNP) and non-SNP alleles. T ...
Study of the evolution of animal parasite bacteria and plant symbionts
Study of the evolution of animal parasite bacteria and plant symbionts

... The alpha-proteobacteria include various microorganisms of biological and medical interest. Some of them are agents of human diseases such as typhus or cat-scratch disease, others are plants symbionts that enter plant roots and live inside it in a cooperative manner, each partner drawing benefit fro ...
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The evolution of life science methodologies: From single gene

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New Study Reveals Power of Family History to Identify 17 New

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Whole genome sequencing



Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.
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