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Molecular genetics
Molecular genetics

... group) is added to the 5’ end of RNA after splicing. RNA cap determines the site of translation. PolyA tailing is the process by which a long tail of Adenine residue is added to the 3’ end of m-RNA during splicing. Ribozymes are RNA molecules act as enzymes. RNase P is a Ribozyme. 9. Recombinant DNA ...
Lab 11: DNA Testing
Lab 11: DNA Testing

- Cal State LA - Instructional Web Server
- Cal State LA - Instructional Web Server

DNA profiling - Our eclass community
DNA profiling - Our eclass community

... Biotechnology is using living things to create products or to do tasks for human beings. It is the practice of using plants, animals and micro-organisms and ...
Frequently Asked Questions.
Frequently Asked Questions.

... DNA can be regarded as a recipe for the substances that our body creates. At InsightYou, we are predominantly interested in the DNA that contributes to substances that influence our brain cells. Variations in DNA mean, for instance, that a certain type of brain cell can be more (or less) active than ...
Who Killed Esmeralda Gooch
Who Killed Esmeralda Gooch

... Late one night, the famous rock star, Esmeralda Gooch, returned to her luxurious apartment from an appearance at a concert. As she entered her locked apartment, she noticed that everything in her apartment was a mess; the drawers had been emptied out onto the floor, the cushions in the couch were ri ...
Recombinant Biotechnology
Recombinant Biotechnology

CPW_PRES - Cumberland Plain Seeds
CPW_PRES - Cumberland Plain Seeds

Determination of the pH Scale by the Method of
Determination of the pH Scale by the Method of

... A lot of research is being done on molecules that bind to DNA. The figure above shows one common binding mode, in which the molecule sticks into a groove of DNA. The binding is especially interesting if it is “sequence specific”, such that the molecule binds only to specific sequences of DNA base pa ...
DNA Extraction from …
DNA Extraction from …

... • The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. • The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA does not denature (break up). ...
No Slide Title
No Slide Title

DNA REPLICATION Review of DNA Structure
DNA REPLICATION Review of DNA Structure

... • The sugar-phosphate backbones of the double helix strands run antiparallel to each other – Each DNA strand has a 3’ end with a free hydroxyl group – and a 5’ end with a free phosphate group – One strand runs 5’ to 3’ and the other runs 3’ to 5’ ...
short_answer_Barcoding_exam_Key
short_answer_Barcoding_exam_Key

... Yes, if the 3rd base pair is changed it is not likely to alter the amino acid, so often times it is a good identification source of a species. However, sometimes when a nucleotide changes it changes the amino acid and can alter the protein, but do not always impair function 13. a. What does the COX1 ...
Supplementary Methods, Figures and Tables This file contains
Supplementary Methods, Figures and Tables This file contains

... Choice of pairs of isolates suitable for quantitative molecular analyses A major constraint in choosing isolates out of the pool of 18 was to choose pairs that could be distinguished by quantifying a small number of the 13 possible molecular markers. Only a very small number of the markers can be u ...
Basic Steps of the DNA process
Basic Steps of the DNA process

... individual. This technique had a very high power of discrimination per loci however it required a large  amount of high quality DNA sample. As the polymerase chain reaction (PCR) was developed a new  technique known as Short Tandem Repeat (STR) became the new standard for DNA analysis. PCR/STR  has  ...
Shotgun sequencing
Shotgun sequencing

... then synthesize a new primer near the end of the known sequence; and repeat. Works, but at best you’d be able to sequence maybe 500 bases a day—making it impossible to sequence something like the human genome, with its billions of bases. Another approach, used to sequence very large amounts of DNA ( ...
Heidi Sleister
Heidi Sleister

... The Case of the Missing Strawberries: RFLP Analysis Microsoft Clipart picture of strawberries ...
lab- where`s the CAT palffy 2010-1
lab- where`s the CAT palffy 2010-1

... DNA restriction enzymes cut the DNA into smaller pieces. These enzymes only cut the DNA at specific places based upon specific sequences of nucleotides. Theses fragments of DNA (known as RFLPs –Restriction Fragment Length Polymorphism) are placed into wells of an electrophoretic gel and the differen ...
2009a Population genomics and the bacterial species concept_002
2009a Population genomics and the bacterial species concept_002

CP Biology 9.2 Copying DNA PCR uses polymerase to copy DNA
CP Biology 9.2 Copying DNA PCR uses polymerase to copy DNA

... DNA might be used to make a DNA fingerprint. The more regions that are used, the less likely it is that two people will have the same DNA fingerprint. There is a very small change – in in many million – that two people have the same DNA fingerprint. DNA fingerprinting is used for many different purp ...
Repetitive DNA info - A. Prokaryotes Eukaryotes Most codes for
Repetitive DNA info - A. Prokaryotes Eukaryotes Most codes for

... with more than about 230 copies are affected by the disease. The low number of repeats found in normal individuals is stable but the intermediate number of copies, found in carriers, is unstable and is said to be a premutation allele. When this allele passes through the male germ line there is no c ...
Slide 1
Slide 1

... • Use PCR to amplify microsatellite products at 7 loci (repeated twice) • Run on agarose gel to confirm success of amplification and to determine amount required for sequencing • Run on sequencer • Analyse using GeneMapper software and by eye ...
DNA fingerprinting and the 16S
DNA fingerprinting and the 16S

... DNA fingerprinting and the bacterial 16S-23S rRNA intergene region. Relationships among bacteria have traditionally been examined using a variety of morphological (staining), biochemical and serological procedures and grouping together those bacteria that share the greatest number of traits. The res ...
Analysis of Gene Sequences
Analysis of Gene Sequences

... (1) A crude preparation of chromosomal DNA is extracted from the bacterial strain of interest. (2) Two short oligo nucleotide primers (each about 18 bases long) are added to the DNA. The primers are designed from the known genomic sequence to be complimentary to opposite strands of DNA and to flank ...
Click on Revolution
Click on Revolution

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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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