Restriction Enzyme
... - Detection of pathogen (bacteria and virus) - Detection of cancers (mutations of ras genes) 2. Forensics - Some genes are highly variable within a population (human leukocyte antigen type, HLA) 3. Molecular Evolution - DNA is very stable and remain intact for thousands of years or longer, particula ...
... - Detection of pathogen (bacteria and virus) - Detection of cancers (mutations of ras genes) 2. Forensics - Some genes are highly variable within a population (human leukocyte antigen type, HLA) 3. Molecular Evolution - DNA is very stable and remain intact for thousands of years or longer, particula ...
Genetic Engineering
... Use a restriction endonuclease to remove a gene from an organism - Create sticky ends! Use the same restriction endonuclease to cut open the plasmidCreate sticky ends! Insert the gene into the plasmid Place the plasmid back into the bacterium ...
... Use a restriction endonuclease to remove a gene from an organism - Create sticky ends! Use the same restriction endonuclease to cut open the plasmidCreate sticky ends! Insert the gene into the plasmid Place the plasmid back into the bacterium ...
Cloning and Gene Therapy
... impossible to clone a mammal • In 1997 a sheep was successfully cloned • Since then cows, pigs, mice and other mammals have been cloned • Cloned animals may suffer from genetic defects and health problems ...
... impossible to clone a mammal • In 1997 a sheep was successfully cloned • Since then cows, pigs, mice and other mammals have been cloned • Cloned animals may suffer from genetic defects and health problems ...
Chapter 14 Genetic Engineering PP Notes
... Recombinant DNA technology • DNA from different organisms is spliced together • Allows scientists to make many copies of any DNA segment (clone) • Can introduce foreign DNA into cells of microorganisms ...
... Recombinant DNA technology • DNA from different organisms is spliced together • Allows scientists to make many copies of any DNA segment (clone) • Can introduce foreign DNA into cells of microorganisms ...
Chapter Three The Biological Basis of Life
... Referring to the fact that DNA bases form base pairs in a precise manner. For example, adenine can bond only to thymine. Thus, these two bases are said to be complementary because one requires the other to form a complete DNA base pair. ...
... Referring to the fact that DNA bases form base pairs in a precise manner. For example, adenine can bond only to thymine. Thus, these two bases are said to be complementary because one requires the other to form a complete DNA base pair. ...
Chapter 16 Review
... complementary to each other, they can be joined together, A. even though the source of the DNA is different B. even though the source of the DNA is the same C. but the “sticky ends” will most likely have to be modified ...
... complementary to each other, they can be joined together, A. even though the source of the DNA is different B. even though the source of the DNA is the same C. but the “sticky ends” will most likely have to be modified ...
Name: Chapter 8 DNA Study Guide There are two main nucleic
... 20. ___________________________ (rRNA) binds to the mRNA and uses the instructions to assemble amino acids in the correct order 21. ___________________________ (tRNA) is the supplier. Transfer RNA delivers amino acids to the ribosome to be assembled into a protein 22. In the nucleus, enzymes make a ...
... 20. ___________________________ (rRNA) binds to the mRNA and uses the instructions to assemble amino acids in the correct order 21. ___________________________ (tRNA) is the supplier. Transfer RNA delivers amino acids to the ribosome to be assembled into a protein 22. In the nucleus, enzymes make a ...
Slide 1 - Montville.net
... Vectors can be used to create Genetically Modified Organisms Organisms that have acquired genes by artificial means . If organism has two different species of DNA, it is a transgenic. Vectors can be used to mass produce gene products (proteins) Examples of proteins are insulin, human growth hormone, ...
... Vectors can be used to create Genetically Modified Organisms Organisms that have acquired genes by artificial means . If organism has two different species of DNA, it is a transgenic. Vectors can be used to mass produce gene products (proteins) Examples of proteins are insulin, human growth hormone, ...
Chapter 20 – DNA Technology - Fort Thomas Independent Schools
... 5. One feature of “engineered” plasmids that is helpful in the isolation and analysis of cloned DNA is: a) they can only handle DNA fragments of up to 120 kb b) that they are an integral part of all eukaryotic cells c) they contain no genetic material of their own so that the cloned fragment is trul ...
... 5. One feature of “engineered” plasmids that is helpful in the isolation and analysis of cloned DNA is: a) they can only handle DNA fragments of up to 120 kb b) that they are an integral part of all eukaryotic cells c) they contain no genetic material of their own so that the cloned fragment is trul ...
Chapter 20 - BEHS Science
... –They grow quickly like bacteria –They are eukaryotes (similar enzymes, metabolic mechanisms, protein mods) –They have plasmids (rare for eukaryotes) –Can replicate artificial chromosomes as well as DNA in plasmids ...
... –They grow quickly like bacteria –They are eukaryotes (similar enzymes, metabolic mechanisms, protein mods) –They have plasmids (rare for eukaryotes) –Can replicate artificial chromosomes as well as DNA in plasmids ...
Name____________________________ DNA Investigation
... Use website #2 to answer the following questions after watching the animation: 4—What is the first step of protein synthesis called? 5—What is the second step of protein synthesis called? What happens during this step? 6—What three nitrogen bases make up the “start codon”? ___ ___ ___ 7—What type ...
... Use website #2 to answer the following questions after watching the animation: 4—What is the first step of protein synthesis called? 5—What is the second step of protein synthesis called? What happens during this step? 6—What three nitrogen bases make up the “start codon”? ___ ___ ___ 7—What type ...
Piecing Together an Identity
... Blood Typing vs Secretor Status If the saliva of a secretor is mixed with the antiserum or lectin specific for its blood group substance then most of the antibody in the antiserum will bind to the blood group substance in the saliva. So when you add the red blood cells for that type no clumping or ...
... Blood Typing vs Secretor Status If the saliva of a secretor is mixed with the antiserum or lectin specific for its blood group substance then most of the antibody in the antiserum will bind to the blood group substance in the saliva. So when you add the red blood cells for that type no clumping or ...
Product Datasheets
... PCR, verify the PCR products by gel electrophoresis. If multiple bands are obtained, gel purify your DNA insert fragment. Be sure to elute the DNA fragments from column using water. ✔ When gel purifying DNA fragments, employ extra caution to minimize any potential damage to the ends of the DNA fragm ...
... PCR, verify the PCR products by gel electrophoresis. If multiple bands are obtained, gel purify your DNA insert fragment. Be sure to elute the DNA fragments from column using water. ✔ When gel purifying DNA fragments, employ extra caution to minimize any potential damage to the ends of the DNA fragm ...
Slide 1
... The Structure of DNA At the same time that Franklin was doing her work, two English scientists were also determined to find the structure of DNA. Francis Crick and James Watson built 3-D models of DNA. Watson observed Franklin’s X-ray pattern of DNA and thus realized the importance of it. Through b ...
... The Structure of DNA At the same time that Franklin was doing her work, two English scientists were also determined to find the structure of DNA. Francis Crick and James Watson built 3-D models of DNA. Watson observed Franklin’s X-ray pattern of DNA and thus realized the importance of it. Through b ...
Document
... They used bacterial plasmids which are small circular replicating fragments of DNA They also used enzymes that cut DNA into specific fragments. These enzymes are called restriction endonucleases (enzymes ...
... They used bacterial plasmids which are small circular replicating fragments of DNA They also used enzymes that cut DNA into specific fragments. These enzymes are called restriction endonucleases (enzymes ...
Horizontal Gene Transfer
... Transduction: bacterial DNA transferred by viruses (phage) Conjugation: DNA transfer between bacterial cells ...
... Transduction: bacterial DNA transferred by viruses (phage) Conjugation: DNA transfer between bacterial cells ...
EXAM 2
... 22. ___T___ For most diploid eukaryotic organisms, sexual reproduction is the only mechanism resulting in new members of a species. 23. ___T___ In C. elegans, the male phenotype is determined by the presence of one X chromosome. 24. ___T___ If a human is monosomic X, the individual will be female. 2 ...
... 22. ___T___ For most diploid eukaryotic organisms, sexual reproduction is the only mechanism resulting in new members of a species. 23. ___T___ In C. elegans, the male phenotype is determined by the presence of one X chromosome. 24. ___T___ If a human is monosomic X, the individual will be female. 2 ...
Genom
... Modificated histons are bound to other types proteins - system readers and writers Histon code is second level of genome information realization, genetic code is the first level ...
... Modificated histons are bound to other types proteins - system readers and writers Histon code is second level of genome information realization, genetic code is the first level ...
Chapter 9 – DNA-Based Information Technologies
... overhang of ssDNA on each strand blunt ends – cut directly across both strands leaving all dsDNA ...
... overhang of ssDNA on each strand blunt ends – cut directly across both strands leaving all dsDNA ...
No Slide Title
... • Utilizes microbiological selection and screening procedures to isolate a gene that represents as little as 1 part in a million of the genetic material in an organism. ...
... • Utilizes microbiological selection and screening procedures to isolate a gene that represents as little as 1 part in a million of the genetic material in an organism. ...
Topics covered on this exam include: cellular respiration
... 1. Compare and contrast DNA with RNA. How do they differ structurally? How are their functions different? 2. What are the components of a single nucleotide? Dow we find nucleotides in both RNA and DNA? 3. Be able to go between DNA DNA, DNA RNA and RNA RNA. 4. What are the three types of RNA? W ...
... 1. Compare and contrast DNA with RNA. How do they differ structurally? How are their functions different? 2. What are the components of a single nucleotide? Dow we find nucleotides in both RNA and DNA? 3. Be able to go between DNA DNA, DNA RNA and RNA RNA. 4. What are the three types of RNA? W ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.