Guided notes 2013 Sections 1 and 2 KEY
... Step 1: In a Southern blot, the DNA from each bacterial clone colony is isolated and cut into fragments by restriction enzymes. Step 2: The DNA fragments are separated by gel electrophoresis, a technique that uses an electric field within a gel to separate molecules by their size. Step 3: The DNA ba ...
... Step 1: In a Southern blot, the DNA from each bacterial clone colony is isolated and cut into fragments by restriction enzymes. Step 2: The DNA fragments are separated by gel electrophoresis, a technique that uses an electric field within a gel to separate molecules by their size. Step 3: The DNA ba ...
PAN Shen Quan
... including plant, yeast, fungal and human cells. This DNA transfer represents the only known example of interkingdom transfer of genetic information. We adopt a molecular genetic approach to identify both bacterial and eukaryotic genes responsible for the transfer process. With a combination of molec ...
... including plant, yeast, fungal and human cells. This DNA transfer represents the only known example of interkingdom transfer of genetic information. We adopt a molecular genetic approach to identify both bacterial and eukaryotic genes responsible for the transfer process. With a combination of molec ...
Aim: What is the structure of the DNA molecule?
... Chromosomes are found in the nucleus of a cell. (Therefore DNA is in the nucleus) There are 46 pairs of chromosomes in the human cell. DNA is an instruction manual for all the processes that the organism does. DNA has all the information needed to make an entire individual. Everyone's DNA is unique ...
... Chromosomes are found in the nucleus of a cell. (Therefore DNA is in the nucleus) There are 46 pairs of chromosomes in the human cell. DNA is an instruction manual for all the processes that the organism does. DNA has all the information needed to make an entire individual. Everyone's DNA is unique ...
Transcription is the process by which RNA polymerase copies a
... Y they stained another substance called RNA. The picture below shows a group of cells that were stained with both DAPI (blue) and Pyronin Y (Red in high concentrations, orange in low concentrations) ...
... Y they stained another substance called RNA. The picture below shows a group of cells that were stained with both DAPI (blue) and Pyronin Y (Red in high concentrations, orange in low concentrations) ...
Defined - cloudfront.net
... • Somatic cell mutations – Affect only the individual – Not passed on to future generations – Ex: Muscle cell mutation • Germ cell mutations – Germ cells = the diploid cells that undergo meiosis to make sperm & egg – May be passed to future generations ...
... • Somatic cell mutations – Affect only the individual – Not passed on to future generations – Ex: Muscle cell mutation • Germ cell mutations – Germ cells = the diploid cells that undergo meiosis to make sperm & egg – May be passed to future generations ...
de novo
... complex biological molecules and systems that is difficult to obtained from ensemble. ...
... complex biological molecules and systems that is difficult to obtained from ensemble. ...
DNA cloning yields multiple copies of a gene or
... electricity, called electroporation, or a Ca2+ containing solution. After transformation, how do scientists know any plasmid, recombinant or not, has been taken up by the bacteria? ...
... electricity, called electroporation, or a Ca2+ containing solution. After transformation, how do scientists know any plasmid, recombinant or not, has been taken up by the bacteria? ...
DNA Polymerase
... sequence in which they are linked together determines the proteins function. Change the sequence, type, or number of amino acids in a protein you change the function. Amino Acids without water sensitive R-groups ...
... sequence in which they are linked together determines the proteins function. Change the sequence, type, or number of amino acids in a protein you change the function. Amino Acids without water sensitive R-groups ...
Unit 3
... 16. Know the basic structure of DNA in terms of the three fundamental building blocks (nitrogenous base, five-carbon sugar, phosphate group), and how those building blocks go together to make a polymer. 17. Know how hydrogen bonds hold a DNA molecule together and how the pattern of hydrogen bonding ...
... 16. Know the basic structure of DNA in terms of the three fundamental building blocks (nitrogenous base, five-carbon sugar, phosphate group), and how those building blocks go together to make a polymer. 17. Know how hydrogen bonds hold a DNA molecule together and how the pattern of hydrogen bonding ...
File - Intermediate School Biology
... 3. Diagnostic test for changed genes 4. (a) Shields the –ve DNA from the +ve proteins causing the DNA to clump. (b) Inactivates any enzymes not denatured.(c) removes cellular debris ( cell walls and membranes) (d) removes the protein associated with DNA. (e) DNA is insoluble in ice cold ethanol and ...
... 3. Diagnostic test for changed genes 4. (a) Shields the –ve DNA from the +ve proteins causing the DNA to clump. (b) Inactivates any enzymes not denatured.(c) removes cellular debris ( cell walls and membranes) (d) removes the protein associated with DNA. (e) DNA is insoluble in ice cold ethanol and ...
Unit 5 Review
... 13. Number the steps of DNA replication in the correct order (1, 2, 3) _______ Daughter strands are formed using complementary base pairing. _______ DNA unwinds. _______ The DNA of the daughter strands winds with together with its parent strand. 14. Show the complimentary base pairing that would oc ...
... 13. Number the steps of DNA replication in the correct order (1, 2, 3) _______ Daughter strands are formed using complementary base pairing. _______ DNA unwinds. _______ The DNA of the daughter strands winds with together with its parent strand. 14. Show the complimentary base pairing that would oc ...
Recombinant DNA Simulation
... Introduction: One of the most important processes developed by biotechnologists was the procedure where a gene is removed from the DNA of one organism and inserted into the DNA of another organism. This technique is called Recombinant DNA. The entire procedure is dependent upon using the correct res ...
... Introduction: One of the most important processes developed by biotechnologists was the procedure where a gene is removed from the DNA of one organism and inserted into the DNA of another organism. This technique is called Recombinant DNA. The entire procedure is dependent upon using the correct res ...
Molecular Pathology - Charles River Laboratories
... fluorescence-based technology that enables pathologists to provide rapid quantitative results on the distribution and expression of target DNA or RNA from a variety of sample types over a broad range of diseases. At Charles River, Q-PCR is used as a primary end point to analyze biodistribution and e ...
... fluorescence-based technology that enables pathologists to provide rapid quantitative results on the distribution and expression of target DNA or RNA from a variety of sample types over a broad range of diseases. At Charles River, Q-PCR is used as a primary end point to analyze biodistribution and e ...
Biology Spring Semester Final Exam Review
... 27. Know patterns of inheritance for genetic disorders like cystic fibrosis and Huntington’s disease. 28. What do restriction enzymes do and why are they important for studying the human genome? 29. What is the Human Genome Project? Ch. 15 30. What is inbreeding and why can it be disadvantageous? 31 ...
... 27. Know patterns of inheritance for genetic disorders like cystic fibrosis and Huntington’s disease. 28. What do restriction enzymes do and why are they important for studying the human genome? 29. What is the Human Genome Project? Ch. 15 30. What is inbreeding and why can it be disadvantageous? 31 ...
Genetic engineering
... Bacterial DNA Bacteria contains plasmids- small rings of DNA separate from the bacterium’s larger circular chromosome The foreign DNA is inserted into the plasmid by cleaving both using the same restriction enzyme Sticky ends match up and foreign DNA becomes part of plasmid ...
... Bacterial DNA Bacteria contains plasmids- small rings of DNA separate from the bacterium’s larger circular chromosome The foreign DNA is inserted into the plasmid by cleaving both using the same restriction enzyme Sticky ends match up and foreign DNA becomes part of plasmid ...
Topic 12 DNA Technology
... 2. Obtain a plasmid (circular DNA) from bacteria 3. Use restriction enzymes to cut out the gene of interest and to cut the plasmid to receive the gene 4. Mix source DNA gene with plasmid to form recombinant DNA 5. DNA ligase will seal the phosphodiester bonds 6. Insert recombinant DNA into host cell ...
... 2. Obtain a plasmid (circular DNA) from bacteria 3. Use restriction enzymes to cut out the gene of interest and to cut the plasmid to receive the gene 4. Mix source DNA gene with plasmid to form recombinant DNA 5. DNA ligase will seal the phosphodiester bonds 6. Insert recombinant DNA into host cell ...
Genetic Information DNA - Barnegat Township School District
... • Results in the wrong base pair sequence • Can cause serious damage – wrong amino acid – protein non functional • Can be silent – no change in amino acid, no change in protein: - UUU changed to UUC – both are codons for the same amino acid Phenylalanine ...
... • Results in the wrong base pair sequence • Can cause serious damage – wrong amino acid – protein non functional • Can be silent – no change in amino acid, no change in protein: - UUU changed to UUC – both are codons for the same amino acid Phenylalanine ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.