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GEN2MHG – MOLECULAR AND HUMAN GENETICS DNA is made
GEN2MHG – MOLECULAR AND HUMAN GENETICS DNA is made

... more accessible to transcription factors Alternative DNA structures; ▪ B-DNA – most common and biologically significant -> right handed and 10 base pairs per turn ▪ A-DNA – occurs in dehydrated laboratory conditions, right handed, 11bp per turn ▪ Z-DNA – short lived, left handed structure, 12bp per ...
powerpoint notes
powerpoint notes

... When RNA polymerase reaches the terminator region on the DNA it will stop and release the mRNA ...
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CHAPTER 17 RECOMBINANT DNA AND BIOTECHNOLOGY
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... 4. Human identical twins are clones; the original single embryo separate to become two individuals. 5. Gene cloning is production of many identical copies of the same gene. 6. If the inserted gene is replicated and expressed, we can recover the cloned gene or protein product. 7. Cloned genes have ma ...
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... 2. The blue-white screen for recombinant plasmids involves the tetracyclin-resistance gene. F 3. Southern blotting is used for the analysis of total RNA. F 4. DNA fingerprinting in forensic science and in paternity tests makes use of VNTRs. T 5. SNPs enable the most refined mapping of genes on chrom ...
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... The start codon for protein biosynthesis is AUG, which is the same as the codon for methionine. Thus, all proteins initially have methionine as their N-terminal amino acid, but lose it subsequent to their formation. The reaction responsible for extending the protein chain is nucleophilic acyl substi ...
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... 2. Enzymes cut the DNA at the specific site of the desirable gene to remove it 3. Enzymes cut the DNA of the other organism to make a gap 4. The gene is transplanted into the gap in the DNA 5. The DNA is sealed together ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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