• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Human Cells Summary
Human Cells Summary

... (c) Replication of DNA by DNA polymerase and primer. DNA is unwound and unzipped to form two template strands. DNA polymerase needs a primer to start replication and can only add complementary DNA nucleotides to the deoxyribose (3') end of a DNA strand. This results in one strand being replicated co ...
Powerpoint - Wishart Research Group
Powerpoint - Wishart Research Group

... Performs 330 trillion operations per second A spoonful contains 15,000 trillion “computers”/automatons Energy-efficiency is more than a million times that of a PC Guinness World Records recognized the computer as "the smallest biological computing device" ever constructed DNA acts as software, enzym ...
Supplementary Materials: Immobilization of Genetically
Supplementary Materials: Immobilization of Genetically

... verified  by  their  digestion  with  diagnostic  restriction  endonucleases  and  confirmed  by  DNA  sequencing (BGI Tech). The result of this process was a (VPGXG)40 insert in the pUC‐57 vector. A  subsequent additional round of recursive directional ligation proceeded identically for (VPGXG)60.  ...
Slide 1
Slide 1

... Recall: Chromosomes, Genes, and DNA •DNA makes up GENES, which are found on CHROMOSOMES. •Gene— a segment of DNA that provides information for making a specific protein. ...
STR DNA Typing: Increased Sensitivity and Efficient Sample
STR DNA Typing: Increased Sensitivity and Efficient Sample

THE GENOMIC SEQUENCING TECHNIQUE George M. Church and
THE GENOMIC SEQUENCING TECHNIQUE George M. Church and

Gene Expression Worksheet
Gene Expression Worksheet

... 2. Where does the replication of DNA occur inside the cell and what part of the cell cycle? ...
Stem cells - Plain Local Schools
Stem cells - Plain Local Schools

... B. Eukaryotic DNA includes promoter sequences before the point that transcription takes place C. Transcription factors- regulate transcription by binding to promoters or RNA polymerases D. Transcription factors are activated and deactivated by certain chemical signals in the cell 1. Hormones may att ...
File
File

...  Genetic information is stored and passed to subsequent generations through DNA molecules and, in some cases, RNA molecules.  Prokaryotes, viruses and eukaryotes contain plasmids, which are small extra chromosomal, double stranded circular DNA molecules ...
DNA Replication
DNA Replication

... • To make new cells and make sure all new cells have a complete set of the genetic info ...
genetically modified organism (GMO)
genetically modified organism (GMO)

... applications aimed at improving human health (e.g., gene therapy) or agriculture (e.g., golden rice). The term "genetically modified organism" does not always imply, but can include, targeted insertions of genes from one into another species. For example, a gene from a jellyfish, encoding a fluoresc ...
壹 - 國立彰化師範大學圖書館
壹 - 國立彰化師範大學圖書館

... 11. The bacterial RNA polymerase consists of a core enzyme (2’) and a sigma factor (). If we treated the enzyme with an antibiotic (rifamycin), the activity of a subunit  was inhibited by preventing initiation of transcription, prior to the formation of the first phospodiester bond. So the func ...
DNA Arrays
DNA Arrays

... • Minisatellites (long sequence repeats), • Deletions, • Duplications. ...
Genetics Review
Genetics Review

Molecular Markers - Personal Web Pages
Molecular Markers - Personal Web Pages

... 23 pairs of chromosomes 3 X 109 base pairs of DNA Different families of repeated sequences Sex chromosomes (X and Y) ...
AQA Biology - Centre of the Cell
AQA Biology - Centre of the Cell

... 3.4.1 DNA, genes and chromosomes In the nucleus of eukaryotic cells, DNA molecules are very long, linear and associated with proteins, called histones. Together a DNA molecule and its associated proteins form a chromosome. A gene is a base sequence of DNA that codes for: • the amino acid sequence of ...
Genetics final exam honors 2010
Genetics final exam honors 2010

... ______________________________ 8. The final stage of cell division where the cytoplasm divides and cells become separate from one another. _______________________________ 9. A change in a gene or chromosome. _______________________________ 10. Condensed DNA typically found in the shape of an X. ____ ...
Chapter 14: Human Heredity - Southington Public Schools
Chapter 14: Human Heredity - Southington Public Schools

...  Explain why most harmful genetic disorders are inherited through recessive alleles.  Identify the heredity and symptoms for common human genetic disorders such as Tay-Sachs, hemophilia, Cystic Fibrosis, Huntington’s disease and color blindness. Chapter 15: Genetic Engineering You should be able t ...
geneticsresearchmoleculargens
geneticsresearchmoleculargens

... techniques we did not do at the lab. Even though I will not start using these notes full-bore (NO PUN INTENDED) until the first Monday in November, it is important that you have them with you. But I do not want to lose ground, or to rush. We will hold class in room 411 from Nov 27-30, 2012 to addres ...
DNA cloning
DNA cloning

... and λ-phage cloning. In this method, called cosmid cloning, recombinant plasmids containing inserted fragments up to a length of 45 kb can be efficiently introduced into E. coli cells. A cosmid vector is produced by inserting the cos sequence from λ-phage DNA into a small E. coli plasmid vector abou ...
cDNA Library, Human HeLa Cell
cDNA Library, Human HeLa Cell

Ch. 9: Presentation Slides
Ch. 9: Presentation Slides

... 3. The vector contains a multiple cloning site (MCS), or polylinker, with unique cleavage sites for many different restriction enzymes that enables many types of restriction fragments to be inserted 4. Cells containing the vector can usually be selected by a straightforward assay, most conveniently ...
Chap 3
Chap 3

... for proteins 3. The human genome has significantly more duplicated segments within it that other mammalian genomes do. These sections may be the source of new primatespecific genes. 4. At the time when the draft sequence was published less than 7% of protein families appeared to be vertebrate-specif ...
CH 14 notes - Lincoln Park High School
CH 14 notes - Lincoln Park High School

...  Describe the step-by-step process by which amino acids are added to a growing polypeptide chain  Diagram the overall process of transcription and translation  Describe the major types of mutations and their possible consequences MYP Unit: Central Dogma (DNA) Key Concept: Systems Systems are sets ...
Guide
Guide

... 29. What are the 5 principles to Darwin’s Theory of Natural Selection? There is ______________________within populations. Some variations are ____________________ because they help the organism survive. In each generation, only a few ________________ long enough to reproduce. The organisms that surv ...
< 1 ... 489 490 491 492 493 494 495 496 497 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report