Section 3 - DNA Sequencing
... • The most important parts of the genome are the genes. • Efforts have been made to identify genes out of sequence data. • Expressed sequence tags (ESTs) are short pieces of sequence data that correspond to mRNAs found in cells of the organism. • ESTs are produced by purifying mRNA from cells and th ...
... • The most important parts of the genome are the genes. • Efforts have been made to identify genes out of sequence data. • Expressed sequence tags (ESTs) are short pieces of sequence data that correspond to mRNAs found in cells of the organism. • ESTs are produced by purifying mRNA from cells and th ...
Microbiology
... There are two basic types: - Generalized transduction: Can transfer any gene from a donor to a recipient cell - Specialized transduction: Can transfer only a few closely linked genes between cells ...
... There are two basic types: - Generalized transduction: Can transfer any gene from a donor to a recipient cell - Specialized transduction: Can transfer only a few closely linked genes between cells ...
Comparing DNA
... The gel is sometimes called a matrix since it contains small holes for the DNA to travel through. If the DNA is too large, then it will not be able to fit through the holes. A restriction enzyme recognizes a particular sequence of bases on the DNA helix and cuts the DNA at that point. The DNA pieces ...
... The gel is sometimes called a matrix since it contains small holes for the DNA to travel through. If the DNA is too large, then it will not be able to fit through the holes. A restriction enzyme recognizes a particular sequence of bases on the DNA helix and cuts the DNA at that point. The DNA pieces ...
DNA notes
... •This is still a wide open area of research, but it is becoming clear that there are several ways to do this. Most of the molecular work has been done using plasmids (they are partition into daughter cells too) and we will look at that closely when we discuss plasmids later. •For now look at the sys ...
... •This is still a wide open area of research, but it is becoming clear that there are several ways to do this. Most of the molecular work has been done using plasmids (they are partition into daughter cells too) and we will look at that closely when we discuss plasmids later. •For now look at the sys ...
Central Dogma: Molecular GeneKcs
... From Mendelian Genetics to Molecular Genetics Recall Genetic lessons from BIOL 180: Alleles are associated with phenotype Alleles (different forms of a gene) are inherited Chromosome theory of inheritance chromosomes are composed of genes alleles on different chromosomes assort independently The ...
... From Mendelian Genetics to Molecular Genetics Recall Genetic lessons from BIOL 180: Alleles are associated with phenotype Alleles (different forms of a gene) are inherited Chromosome theory of inheritance chromosomes are composed of genes alleles on different chromosomes assort independently The ...
handout 1
... The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. We perform a number of these classic diagnostic test ...
... The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. We perform a number of these classic diagnostic test ...
Multiple GMO
... 2. Determine the GMO dealing classification 3. Upload completed table in Section 7 of the online form ...
... 2. Determine the GMO dealing classification 3. Upload completed table in Section 7 of the online form ...
Figure 9.8
... – There are two asymmetrical grooves on the outside of the helix • 1. Major groove • 2. Minor groove ...
... – There are two asymmetrical grooves on the outside of the helix • 1. Major groove • 2. Minor groove ...
(Pulse-field Gel Electrophoresis)
... Why/When: PFGE is performed in the surveillance of retail foods and to monitor for outbreaks. When two or more bacterial isolates match in banding pattern, state epidemiologists are notified and they investigate. The investigation involves interviewing patients to determine if they have a common lin ...
... Why/When: PFGE is performed in the surveillance of retail foods and to monitor for outbreaks. When two or more bacterial isolates match in banding pattern, state epidemiologists are notified and they investigate. The investigation involves interviewing patients to determine if they have a common lin ...
GA Milestone Review 1 1 Carbon dioxide and water are converted
... energy storage for hibernating animals. II) Buoyancy in marine animals such as whales. III) Component of cell membranes in all organisms. A) carbohydrates B) lipids C) nucleic acids D) proteins 13 A police detective finds a speck of blood at a crime scene. He knows that there is not enough DNA in th ...
... energy storage for hibernating animals. II) Buoyancy in marine animals such as whales. III) Component of cell membranes in all organisms. A) carbohydrates B) lipids C) nucleic acids D) proteins 13 A police detective finds a speck of blood at a crime scene. He knows that there is not enough DNA in th ...
DNA TECHNOLOGY AND GENOMICS
... incorporation of exogenous DNA into the cells. As a basis for understanding this concept: c. Students know how genetic engineering (biotechnology) is used to produce novel biomedical and agricultural products. d.* Students know how basic DNA technology (restriction digestion by endonucleases, gel el ...
... incorporation of exogenous DNA into the cells. As a basis for understanding this concept: c. Students know how genetic engineering (biotechnology) is used to produce novel biomedical and agricultural products. d.* Students know how basic DNA technology (restriction digestion by endonucleases, gel el ...
Digitally Programmed Cells
... allowed to hang unpaired, which permits a fifth strand to rip away the ...
... allowed to hang unpaired, which permits a fifth strand to rip away the ...
chapter review answers
... Nucleosomes are made up of DNA wrapped around histones. Chromatin is long chains of tightly coiled nucleosomes. 3. Explain the process of replication. When a DNA molecule is replicated, how do the new molecules relate to the old molecule? DNA separates into two strands, then produces two new complem ...
... Nucleosomes are made up of DNA wrapped around histones. Chromatin is long chains of tightly coiled nucleosomes. 3. Explain the process of replication. When a DNA molecule is replicated, how do the new molecules relate to the old molecule? DNA separates into two strands, then produces two new complem ...
chapter 14 15 16 study guide
... Helicase: enzyme that unzips the dna strands Topoisomerase: enzyme that untangles the dna strand upstrand from the origin of replication to keep the DNA helix from tangling Single strand binding protein: protein that holds the single strand of a DNA until it is able to be replicated (adds stability ...
... Helicase: enzyme that unzips the dna strands Topoisomerase: enzyme that untangles the dna strand upstrand from the origin of replication to keep the DNA helix from tangling Single strand binding protein: protein that holds the single strand of a DNA until it is able to be replicated (adds stability ...
PCR
... PCR (Polymerase Chain Reaction) ● PCR ○ 1983 first discovered by Kary Mullis ○ used to amplify a single copy or a few copies of DNA in order to create 1000s or millions of copies of a particular DNA sequence ○ OVERVIEW ■ relies on thermal cycling, consisting of repeated heated and cooling reactions ...
... PCR (Polymerase Chain Reaction) ● PCR ○ 1983 first discovered by Kary Mullis ○ used to amplify a single copy or a few copies of DNA in order to create 1000s or millions of copies of a particular DNA sequence ○ OVERVIEW ■ relies on thermal cycling, consisting of repeated heated and cooling reactions ...
4-1 - GSCS
... Solution – DNA fingerprinting – identify individuals but also determine how closely related they are Pair unrelated individuals to produce offspring – selective breeding to maintain genetic variation Mating unsuccessful – discover why female cannot become pregnant or analyze semen of partner ...
... Solution – DNA fingerprinting – identify individuals but also determine how closely related they are Pair unrelated individuals to produce offspring – selective breeding to maintain genetic variation Mating unsuccessful – discover why female cannot become pregnant or analyze semen of partner ...
DNA Technology
... turning on and off of genes • This means that there are times when DNA is expressed and when it is not expressed • Remember DNA RNA ...
... turning on and off of genes • This means that there are times when DNA is expressed and when it is not expressed • Remember DNA RNA ...
Genetic Engineering
... • We know that mutations are incredibly dangerous, and 9 times out of 10 result in death, so WHY is it that we spend millions of dollars trying to force mutations? • The problem with selective breeding is that it is ALWAYS confined to genes that are already found within a population • Mutations, dan ...
... • We know that mutations are incredibly dangerous, and 9 times out of 10 result in death, so WHY is it that we spend millions of dollars trying to force mutations? • The problem with selective breeding is that it is ALWAYS confined to genes that are already found within a population • Mutations, dan ...
APPLICATIONS
... What is a GENOMIC LIBRARY, What are their limitations? 1. Start with fragments of entire genome of an organism 2. each fragment cloned into vector (plasmid) 3. complete set of plasmids, each carrying a particular segment of the original genome = genomic library limitations a. genomic DNA contain int ...
... What is a GENOMIC LIBRARY, What are their limitations? 1. Start with fragments of entire genome of an organism 2. each fragment cloned into vector (plasmid) 3. complete set of plasmids, each carrying a particular segment of the original genome = genomic library limitations a. genomic DNA contain int ...
Genetics Exam 5
... B. ligase C. restriction endonuclease D. sticky ends E. cDNA _____ DNA termini without overhangs produced by endonuclease digestion are called A. cohesive termini B. sticky ends C. blunt ends D. oligonucleotides E. none of the above _____ Which enzyme functions to synthesize RNA from a DNA template? ...
... B. ligase C. restriction endonuclease D. sticky ends E. cDNA _____ DNA termini without overhangs produced by endonuclease digestion are called A. cohesive termini B. sticky ends C. blunt ends D. oligonucleotides E. none of the above _____ Which enzyme functions to synthesize RNA from a DNA template? ...
86K(a)
... E. none of the above 24.Which one below is not a kind of direct selection method in genetic engineering: A. antibiotic screening B. marker rescue C. in situ hybridization D. nutrition rescue E. enzyme immunodetection assay 25. The sequence acts as modification point in transcription termination in e ...
... E. none of the above 24.Which one below is not a kind of direct selection method in genetic engineering: A. antibiotic screening B. marker rescue C. in situ hybridization D. nutrition rescue E. enzyme immunodetection assay 25. The sequence acts as modification point in transcription termination in e ...
Horizontal Gene Transfer in Prokaryotes
... challenges to humans, animals and plants. Prokaryotes also play a central role as tools for biotechnology. In this lecture we will mainly focus on the group of the Bacteria. Slide 4 Prokaryotes usually reproduce asexually by cell division, also referred to as ‘vertical gene transfer’. The division o ...
... challenges to humans, animals and plants. Prokaryotes also play a central role as tools for biotechnology. In this lecture we will mainly focus on the group of the Bacteria. Slide 4 Prokaryotes usually reproduce asexually by cell division, also referred to as ‘vertical gene transfer’. The division o ...
Chp 11.2: Nucleic Acid structure and sequence
... These errors in DNA can be caused by long-term chemical or radiation exposure. These errors in Gene copies can either replace the correct, normal Genetic Codes with a different Code, or leave out a piece of a trait’s code completely. ...
... These errors in DNA can be caused by long-term chemical or radiation exposure. These errors in Gene copies can either replace the correct, normal Genetic Codes with a different Code, or leave out a piece of a trait’s code completely. ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.