• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
dna
dna

... Steps in Replication 2. A molecule of DNA POLYMERASE binds to one of the strands of DNA and begins to move in the 3’ to 5’ direction along it. This produces a new strand of DNA that is called the LEADING STRAND. DNA in the leading strand is synthesized in the 5’ to 3’ direction which is the ONLY wa ...
Structure of DNA
Structure of DNA

... Selectivity of Primers • Primers bind to their complementary sequence on the target DNA – A primer composed of only 3 letter, ACC, for example, would be very likely to encounter its complement in a genome. – As the size of the primer is increased, the likelihood of, for example, a primer sequence o ...
From Gene To You
From Gene To You

... Promoter: a sequence of DNA which RNA polymerase attaches to begin transcription Operator: a sequence that blocks action of RNA polymerase IF occupied by repressor protein Structural Gene: DNA that codes for several related enzymes that direct production of product ...
Heredity
Heredity

... • New organisms is produced from the combined DNA of TWO different cells called sex cells. – Male is called sperm & Female is called egg • Fertilization occurs when an egg and sperm unite to form a new organism with half of each parent’s DNA • Plants sexually reproduce from male and female parts of ...
Chemistry
Chemistry

... DNA in blue PolyA+RNA in red ...
Application of Molecular Biotechnologies to Remediation
Application of Molecular Biotechnologies to Remediation

... T-RFLP (terminal-restriction fragment length polymorphism): Separates amplified 16S molecules by restriction patterns LH-PCR (length heterogeneity polymerase chain reaction): Separates amplified 16S molecules by length RISA (ribosomal intergenic spacer analysis): Separates amplified 16S-23S intergen ...
Code Breaker - Georgia Tech ISyE
Code Breaker - Georgia Tech ISyE

... other diseases. If just one part of the histeam are investigating the histone code tone code malfunctions, the wrong gene could from a broad perspective, using chrystallograbe silenced. In the case of a growth-regulating gene, phy, while Vertino concentrates on cancer. the result could be tumor form ...
demonstating sequence-specific cleavage by a restriction enzyme
demonstating sequence-specific cleavage by a restriction enzyme

... into subsamples known as fractions. After each step in the purification, every fraction was separately assayed for the ability to cleave P22 DNA. Fractions that contained the enzyme activity were subjected to yet another purification method, and the process was continued until a pure enzyme was obta ...
Unit 2 Lesson 6
Unit 2 Lesson 6

... • During replication, the two strands of DNA separate. • The bases on each side of the molecule are used as a pattern for a new strand. ...
16 ge_vs_sb_NOTES
16 ge_vs_sb_NOTES

... Dog breeders wanted to breed a dog that would run fast but also be born with long, shiny fur, looking for the best characteristics from the parents. ...
Grade 9 Science - hrsbstaff.ednet.ns.ca
Grade 9 Science - hrsbstaff.ednet.ns.ca

... Explain the importance of using precise language in science and technology. (109-14) Illustrate and describe the basic processes of mitosis and meiosis (304-11). Distinguish between sexual and asexual reproduction in representative organisms (305-2) Compare sexual and asexual reproduction in terms o ...
Genetic Profiling using Short Tandem Repeat Analysis
Genetic Profiling using Short Tandem Repeat Analysis

... one allele mismatch.  A positive identification is based on the unlikely probability  that agreement in allele constellations is due to chance alone.  In  an  actual  genotyping  lab,  scientists  extract  DNA  from  cells  found  in  teeth,  bone,  tissue,  blood,  semen,  or  commonly  from  bucca ...
DNA and PROTEIN SYNTHESIS
DNA and PROTEIN SYNTHESIS

... ex) If mRNA codes UUU, t RNA anticodon is AAA and it will get the amino acid PHE ...
Media Release
Media Release

... “While police are using the latest technology to fight crime, the increased use has led to delays,” Mr Achterstraat said. There is a backlog of around 6,400 cases waiting for DNA evidence to be analysed. “It will take more than a year to process this backlog with current resources even if no more ca ...
Bacteria Evolving: - American Museum of Natural History
Bacteria Evolving: - American Museum of Natural History

... researchers were able to compare the DNA of USA300 with the genomes of other strains of S. aureus bacteria. When researchers did that, important differences jumped out at them. The USA300 MRSA had genes that had never been seen before in S. aureus bacteria, including a set of 34 genes called the Arg ...
Classical and Modern Genetics
Classical and Modern Genetics

... • Fidelity in copying information • Specificity in information • Expression of gene via manufacturing of polypeptide leading to protein (e.g., enzyme) • Genetic Code is conserved in evolution – all organisms use the exact same coding process • Example of Genetic Code: laboratory exercise ...
Biology and computers - Cal State LA
Biology and computers - Cal State LA

... is/are the same in all 3 organisms). Start your paragraph as a hypothesis as to which parts are most important, and write your discussion as a defense of your hypothesis. Find out the chromosomal location of the gene that causes sickle cell anemia. Give the name of the gene. State the nucleotide cha ...
INTRODUCTION TO BIOLOGY Course Description This class
INTRODUCTION TO BIOLOGY Course Description This class

... 1) To study the structure, function and organization of cells and cellular organelles. (Midterm I) 2) To explore cellular processes such as cellular respiration, reproduction and communication. (Midterms I and II) 3) To understand the principles governing the genetics of inheritance. (Midterm III) 4 ...
Biological vocabulary glossary, part 1
Biological vocabulary glossary, part 1

... Together, these three elements create a ​nucleotide​ - the basic unit of DNA (and RNA). Consecutive nucleotides hold together by binding the sugar to the phosphate group of the next nucleotide via covalent bonds. The bases on opposing strands are bound together by hydrogen bonds. Adding nucleotides ...
RF cloning: A restriction-free method for inserting target genes into
RF cloning: A restriction-free method for inserting target genes into

... circumvent these limitations, different approaches have been taken to enhance cloning efficiency, such as positive selection, in which only colonies containing plasmids with inserts survive or discrimination on the basis of blue/white screening. Alternative methods, including GatewayR technology (In ...
Biotechnology - Glen Rose FFA
Biotechnology - Glen Rose FFA

... Contribute to Biotechnology? ...
Biotechnology - drzapbiology
Biotechnology - drzapbiology

... Two types of Cut Sites • Sticky Ends • restriction enzyme cuts the two strands in a staggered way • This results in one end of DNA being single-stranded and overhangs the other strand ...
LCHS Biology Quizdom Review
LCHS Biology Quizdom Review

... 8. Which type of RNA molecule is directly involved with creating the amino acid sequence of the Protein at the ribosome? A) B) C) D) ...
Valhalla High School
Valhalla High School

... needed to get to the ribosome. DNA is converted into a single stranded RNA molecule, called mRNA. This process is called transcription. Draw your codon lines to separate the triplets. Using the base pairing rules for DNA to RNA, find the anticodons for the DNA strand first. Then convert that strand ...
File
File

< 1 ... 398 399 400 401 402 403 404 405 406 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report