• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Chapter 14 When Allele Frequencies Stay Constant
Chapter 14 When Allele Frequencies Stay Constant

... 14.4 DNA Profiling is Based on Hardy-Weinberg Assumptions 1. Hardy-Weinberg equilibrium only applies when natural selection is not occurring. It applies to DNA repeats and other sequences that do not alter evolutionary fitness. 2. DNA repeats are found throughout the genome. 3. Copy number variants ...
Provincial Exam Questions
Provincial Exam Questions

... B. helicase C. nuclease D. peptidase 14. Which of the following occurs during complementary base pairing? A. Bonds form between uracil and thymine. B. Bonds form between cytosine and guanine. C. Bonds break between phosphates and sugars. D. Bonds break between amino acids and phosphates. ...
Cell Nucleus Quiz Answers
Cell Nucleus Quiz Answers

Modern Genetics Notes
Modern Genetics Notes

... 1. Messenger RNA (mRNA). It carries messages directly from DNA in the nudeus to the cytoplasm during the making of protein. The triplet nucleotides of mRNA (such as AAC or UUU) are called codons. 2. Transfer RNA (tRNA). It is shaped like a cloverleaf and carries amino acids to the mRNA at the riboso ...
Editorial: Modulating Prokaryotic Lifestyle by DNA
Editorial: Modulating Prokaryotic Lifestyle by DNA

... Within the research in Molecular Biology, one important field along the years has been the analyses on how prokaryotes regulate the expression of their genes and what the consequences of these activities are. Prokaryotes have attracted the interests of researchers not only because the processes taki ...
January 7, 2014 Notes Transcription: process of copying DNA into
January 7, 2014 Notes Transcription: process of copying DNA into

... January 7, 2014 Notes Transcription: process of copying DNA into an RNA template. (Occurs in nucleus) ...
Ch_ 19_2
Ch_ 19_2

... adjust levels of enzymes for synthesis & digestion ...
BTCH Reg Course Rev Sem2
BTCH Reg Course Rev Sem2

... intron ...
DNA (Gene) Mutations
DNA (Gene) Mutations

... Every part of your DNA doesn’t make a protein or trait. ...
Forensic DNA Analysis
Forensic DNA Analysis

... • Each individual will have different numbers of these repeats at each of these spots – the numbers of repeats at each location in you are a random combination of the repeats in your parents • Each of these spots, or loci, are given different names. The most common are: variable number of tandem rep ...
Microbial Metabolism
Microbial Metabolism

... • Disinfection is the process of limiting or inhibiting microbial growth on a surface • Antisepsis is the process of reducing or limiting microorganisms on a living tissue • Sepsis is bacterial contamination • -cide = to kill • -stat = to inhibit ...
DNA: the Genetic Material Chapter 9.1
DNA: the Genetic Material Chapter 9.1

...  When Griffith looked at the blood of the dead mouse he noticed that the living R type had acquired capsules. The harmless R type had transformed into harmful S types. ...
Exam IV 1710_1711 F'01.doc
Exam IV 1710_1711 F'01.doc

... The nucleotide sequence in DNA recognized by an RNA polymerase which indicates that this location is the start of a gene/operon (a transcription unit if you prefer) and to which the RNA polymerase binds is called a/an: a. ...
Genetic engineering NOTES File
Genetic engineering NOTES File

... In the 1980s, the options were a little limited – the human genome had not been sequenced so finding a gene of 153 bases (51 amino acids x 3 bases per amino acid) really was needle in a haystack territory; constructing a DNA base sequence based on the amino acid composition of insulin was possible, ...
The first midterm will consist of 20 four
The first midterm will consist of 20 four

... assumption commonly applied in twin design studies? Should it be? (1 pt) ...
Table 2A. Summary of Genetics Activities Activity 1: Mitosis and
Table 2A. Summary of Genetics Activities Activity 1: Mitosis and

... Activity 6: Forensic DNA Summary of Forensic DNA…How is forensic DNA used? How could forensic DNA be used in finding an answer to the viewer question? ...
DNA, RNA, and Protein Synthesis Notes
DNA, RNA, and Protein Synthesis Notes

... A section of DNA, with many bases that codes for a protein Proteins are the building blocks for organisms…TRAITS ...
DNA, restriction enzymes
DNA, restriction enzymes

... a) In this case, a Southern blot of HindIII-digested genomic DNA is performed, using one entire exon of the wild-type gene as a labelled probe. The probe is hybridized to the nitrocellulose filter at a temperature of 41C. The blot reveals a band at 4.3 kb for the wild-type strain but, for the mutant ...
Replication Study Guide
Replication Study Guide

... • G always binds to C and A always binds to T because they fit with each other both physically and chemically. • The two strands are complementary, so that the composition of one can be determined by examining the other. • They are also oriented in opposite directions. • In the animation of replicat ...
The Cell
The Cell

... parent must have the disease to pass it to their offspring Ex. Huntington’s Disease • Sex linked: A recessive gene that mother’s carry on their X chromosomes pass it to their sons Ex. Hemophilia • Remember!!! Look to see if there is a key describing the chart!!! ...
AP Biology: Unit 3A Homework
AP Biology: Unit 3A Homework

... 20. What is genomic imprinting? Briefly describe the Igf2 example. 21. Which organelles have genes and how are they inherited? ...
RBC seminar Transcriptional repression of DNA repair genes is a
RBC seminar Transcriptional repression of DNA repair genes is a

... metabolic stress as well as oxidative stress, (ii) it is characterized by a stable proliferation arrest, and (iii) it results in the acquisition of a specific secretome. Timely regulated senescence is thought to be beneficial as it exerts tumor suppressive activity, it contributes to wound healing a ...
Protein Synthesis Simulation Lab
Protein Synthesis Simulation Lab

... Part 1: Introduction ...
Academic Biology
Academic Biology

... Evolutionary theory explains the existence of these adapted to different purposes as result of descent with modification from common ancestor ...
Environmental Health: sanitation, water, pesticide and - Wk 1-2
Environmental Health: sanitation, water, pesticide and - Wk 1-2

< 1 ... 383 384 385 386 387 388 389 390 391 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report