• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses

... Natural selection favors bacterial mutants with receptor sites that are no longer recognized by a particular type of phage ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... 4. tRNA: type of RNA molecule that transfers amino acids to ribosomes during protein synthesis 5. transcription: process in which part of the nucleotide sequence of DNA is copied into a complementary sequence in RNA 6. RNA polymerase: enzyme similar to DNA polymerase that binds to DNA and separates ...
Replication 1
Replication 1

... 1. At a nick (free 3’ OH) in the DNA the DNA pol I binds and digests nucleotides in a 5’-3’ direction 2. The DNA polymerase activity synthesizes a new DNA strand 3. A nick remains as the DNA pol I dissociates from the ds DNA. 4. The nick is closed via DNA ligase ...
The title: A Structure for Deoxyribose Nucleic Acid
The title: A Structure for Deoxyribose Nucleic Acid

... polymer such as proteins that are composed of 20 different letters. The date: 2 April 1953. (Note: Also the year of the Hershey-Chase experiment that tested whether DNA or protein was the informational molecule.) The title: A Structure for Deoxyribose Nucleic Acid Note the use of "deoxyribose nuclei ...
DNA sequencing
DNA sequencing

... © 2016 Paul Billiet ODWS ...
Transcription PPT
Transcription PPT

The Genetic Code and Transcription Chapter 12 Honors Genetics
The Genetic Code and Transcription Chapter 12 Honors Genetics

AP Biology Review Chapters 11-12 Review Questions Chapter 11
AP Biology Review Chapters 11-12 Review Questions Chapter 11

... 9. What is the rate of mistakes in DNA synthesis before and after DNA repair enzymes? (Go with # in text on page 233). What causes mutations? 10. What are nucleosomes? 11. With which organism did Beadle and Tatum experiment? What was their conclusion and the reasoning behind it? 12. What did Paulin ...
Lesson Overview
Lesson Overview

... Base pairing in the double helix explained how DNA could be copied, or replicated, because each base on one strand pairs with only one base on the opposite strand. Each strand of the double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. Because e ...
Protein Synthesis Simulation Activity
Protein Synthesis Simulation Activity

... Part 1: Introduction ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... eutherian mammals this requires a random switch and subsequent maintenance of the active and inactive states. Holliday and Pugh discussed these fundamental features in the wider context of development. Both publications proposed that the hemimethylated DNA after replication is a substrate for a main ...
Name: Date: Period:___ Midterm Review: Study Guide # 4 TOPICS
Name: Date: Period:___ Midterm Review: Study Guide # 4 TOPICS

...  Label the following structures in the diagram using the letters given below. A. Original bacteria cell B. Plasmid C. Human DNA D. Plasmid with human DNA E. Reproduced Bacteria Cell ...
FAQs (frequently asked questions) Q.1 What are plasmids? Ans
FAQs (frequently asked questions) Q.1 What are plasmids? Ans

... This was one of the first artificial cloning vectors to be constructed, and is undoubtedly the most widely used cloning vector till now. It is a 4.36kb double stranded cloning vector. This plasmid vector has been put together from fragments ...
Practice Exam Answers
Practice Exam Answers

... 1. DNA helicase disrupts hydrogen bonds and unwinds the helix. 2. DNA gyrase relieves the tension from the unwinding DNA. 3. Replication bubble is formed. 4. Proteins bind to the origin of replication. 5. Single-stranded binding proteins bind to exposed bases. 6. Primase makes RNA primers that bind ...
View ePoster - 2015 AGU Fall Meeting
View ePoster - 2015 AGU Fall Meeting

... euryarchaeote, beta-proteobacteria, gamma-proteobacteria, and a large number of alpha-proteobacteria (mostly rhizobia) • Shared OTU’s between cDNA and DNA further confirmed that presence of active members • D. audaxviator found in both DNA results and cDNA nifH results - it is active in the subsurfa ...
Unit review questions
Unit review questions

... 54. Where does RNA polymerase bind to the DNA it is transcribing? 55.What makes the beginning of a new gene on DNA in eukaryotes? 56. What do promoters mark the beginning of on prokaryotic DNA? 57. When a promoter binds to DNA, What happens to the double helix? 58. Are both strands of DNA copied dur ...
BIOLOGICAL MOLECULES
BIOLOGICAL MOLECULES

... must first be made to respire anaerobically. To do this to the sample of yeast in a boiling tube add a small amount of Diazin green indicator which will turn ................................. when no oxygen is available and then add a thin layer of paraffin oil to stop oxygen entering. The yeast wil ...
RNA - jpsaos
RNA - jpsaos

... How is transcription different from DNA replication? ...
Teacher PowerPoint - UNC Institute for the Environment
Teacher PowerPoint - UNC Institute for the Environment

MASTER SYLLABUS
MASTER SYLLABUS

... explain how crossing over produces recombination between linked genes. perform gene mapping to illustrate the order and distances of genes along a chromosome. compare and contrast DNA and RNA structure. describe how the structure of DNA was determined. explain the determination of DNA as genetic mat ...
Companion PowerPoint slide
Companion PowerPoint slide

... Refers to changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence. Enables a cell/organism to respond to its dynamic external environment during development and throughout life! Epigenetic changes to the genome can be inherited if these changes occur in cell ...
Gene 5102-96
Gene 5102-96

... Describe one example that supports this principle. ...
replicates
replicates

... Cells function according to the information contained in the master code of DNA (i.e., cell cycle, DNA to DNA, and DNA to RNA). ...
unit-4-genetics-transmission-storage
unit-4-genetics-transmission-storage

... • a. Discuss Gregor Mendel’s importance as the “father of genetics”. (STSE, K) • b. Discuss the historical development of scientific understanding of Mendelian genetics, including the importance of statistical analysis, probability and significance. (STSE, K) • c. Distinguish among the mechanisms of ...
DNA
DNA

... • Define genetics, genome, chromosome, gene, genetic code, genotype, phenotype, and genomics. • Describe how DNA serves as genetic information. • Describe the process of DNA replication. • Describe protein synthesis, including transcription, RNA processing, and translation. • Describe the operon mod ...
< 1 ... 359 360 361 362 363 364 365 366 367 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report