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mutations[1]
mutations[1]

... Tautomerism - A base is changed by the repositioning of a hydrogen atom. An example is 5-bromo-deoxyuridine (5BU), which can exist in two tautomeric forms: typically it exists in a keto form (T mimic) that pairs with A, but it can also exist in an enol form (C mimic) that pairs with G. Depurination ...
3.3 How Do You Identify and Clone a Gene of Interest?
3.3 How Do You Identify and Clone a Gene of Interest?

... – Techniques involve analyzing mRNA produced by a tissue – Northern blot analysis • Basic method is similar to Southern blotting • RNA is isolated from a tissue of interest, separated by gel electrophoresis, blotted onto a membrane, and hybridized to a probe – Reverse transcription PCR • Reverse tra ...
Scrotal asymmetry in man and in ancient sculpture
Scrotal asymmetry in man and in ancient sculpture

... were suspended in 0.3 ml trichloroacetic acid (TCA) at 0.3 M, kept at room temperature for 10 min and the concentration of acid soluble sulphydryls measured in the cell-free extract. tions, a higher yield of strand brealcs is generally found in oxic than in anoxic conditions, both for chromosomal an ...
here - IMSS Biology 2014
here - IMSS Biology 2014

... • In some instances, an advantage is conferred when carrying one copy of a deleterious allele, so natural selection will not remove the allele from the population • E.g. allele that causes sickle cell anemia is deleterious if you carry two copies of it, but carrying one copy confers malaria resistan ...
dominant gene
dominant gene

... adding specific characteristics that give the plant new benefits. For example, biotech rice contains more iron than regular rice. 2. A specific gene is taken from one plant and placed into the DNA of a second plant. The second plant will then grow with all its own traits plus the one from the first ...
CHNOPS Lab
CHNOPS Lab

... Then the mRNA carries this information in the form of a code to the ribosomes, where protein synthesis takes place. The code, in DNA or mRNA, specifies the order in which the amino acids are joined together to form a polypeptide. As the code carried by mRNA is “read” on a ribosome, the amino acids a ...
Gene Regulation
Gene Regulation

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PCR-based Detection of Silkworm Diseases
PCR-based Detection of Silkworm Diseases

Molecular Biology of the Cell
Molecular Biology of the Cell

... Critical step: Strand invasion by RecA • ATP-bound RecA binds single stranded DNA holding it in an elongated form (groups of nucleotide triplets separated) • Protein-DNA filaments binds to dsDNA and stretches it destabilizing the helix • Invading strand probes the DNA for complementary regions usin ...
Supporting Information. Molecular diagnosis of Usher syndrome
Supporting Information. Molecular diagnosis of Usher syndrome

8.4 Transcription
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... • The two processes have different end results. – Replication copies all the DNA; one gene growing RNA strands transcription copies a gene. – Replication makes DNA one copy; transcription can make many copies. ...
Introduction to molecular biology
Introduction to molecular biology

... genes correspond perfectly to the DNA sequences of the genome (with the exception of uracil, which is used in place of thymine), and the steps of transcription and translation are partially overlapped In eukaryotes, the two phases of gene expression are physically separated by the nuclear membrane: ...
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... How does Dr. K “look” at figures? Figure out the experimental rationale, design Make predictions Look at figures – what are the results? Predictions VS Results -> Do I believe it? Missing information? Data? Controls? Each individual figure <-> Main purpose ...
ATP - mustafaaltinisik.org.uk
ATP - mustafaaltinisik.org.uk

... • Inhibits DNA synthesis without affecting RNA synthesis or other nucleotide pools • Cleared from the body rapidly so not used extensively in the clinic ...
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Summary notes for ch1-6

... - The enormous amount of data produced by DNA and protein sequencing can be managed and analysed using computer technology and shared over the internet. - Computer programs can be used to identify gene sequences by looking for coding sequences similar to known genes, start sequences or sequences lac ...
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... Cilia of windpipe cells. The cells that line the human windpipe are equipped with cilia that help keep the lungs clean by moving a film of debris-trapping mucus upward. ...
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FP-123

... When repeatedly reading DNA along the linear range of spectrophotometer, the OD at A260 and the standard deviation will increase. Similarly, as repetitive readings are made at decreasing ODs of A260 the standard deviation will increase. Graphically this is a parabolic function indicating the linear ...
CPS 302 Society, Science, and Technology
CPS 302 Society, Science, and Technology

... for new antibiotics. This year's three Laureates have all generated 3D models that show how different antibiotics bind to the ribosome. These models are now used by scientists in order to develop new antibiotics, directly assisting the saving of lives and decreasing humanity's suffering. ...
Gene Regulation III Reminder
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... molecules to regulate gene expression • 2 sources of RNA ‣ cell’s own genes - MicroRNA (miRNA) ‣ viruses and transposons - Small Interfering RNA (siRNA) • Process likely evolved to inactivate viral genes and transposons ...
BIOL 105 S 2013 Practice Quiz Supp DNA
BIOL 105 S 2013 Practice Quiz Supp DNA

... Which of the following tasks is not accomplished by DNA? A) undergoes mutations that can provide variation B) provides energy for the cell C) stores information D) replicates to pass a copy to the next generation Answer B Which of the following statements is incorrect concerning deoxyribonucleic aci ...
DNA, The Genetic Material
DNA, The Genetic Material

... experienced the transformation. They concluded that a nucleic acid of deoxyribose type was the fundamental unit of the transforming principle; in essence, DNA is the hereditary material. The Hershey-Chase Experiment – Avery’s result was not widely accepted at first; many still believed that proteins ...
Camp 1 - UCSC Directory of individual web sites
Camp 1 - UCSC Directory of individual web sites

... • Triplets of bases on mRNA are called codons. e.g. AUG • The 20 amino acids are then brought to the mRNA-ribosome complex, each amino acid by its own particular tRNA (e.g. w/ Met). ...
DNA Mutations - Cloudfront.net
DNA Mutations - Cloudfront.net

... – Mutations provide genetic variation in species which increase their chances of survival – Some mutations can fix genetic problems if they alter a diseased gene and make it normal – EX. Changes in the gene for bone density can cause individuals to have increased bone density ...
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... 1. Compare the steps of transcription and translation to the processes that take place within a cookie factory. Make analogies for each step along the way. 2. If you are made up of thousands of unique proteins in your body, what conclusions can you make about your DNA sequence? What could happen if ...
Lecture 13
Lecture 13

... In Southern blotting, DNA fragments from transgenic plants generated by digestion with restriction enzyme(s) are first separated according to fragment size by electrophoresis through an agarose gel. The DNA fragments then are transferred to a solid support, such as a nylon membrane or a nitrocellulo ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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