Summer 2003 Test 3
... 19) Which of the following tools of recombinant DNA technology is incorrectly paired with its use? a) restriction enzyme and production of RFLPs b) DNA ligase and enzyme that cuts DNA, creating the sticky ends of restriction fragments c) DNA polymerase and its use in a PCR to amplify sections of DNA ...
... 19) Which of the following tools of recombinant DNA technology is incorrectly paired with its use? a) restriction enzyme and production of RFLPs b) DNA ligase and enzyme that cuts DNA, creating the sticky ends of restriction fragments c) DNA polymerase and its use in a PCR to amplify sections of DNA ...
Clone Unstable DNA by Lowering the Copy Number of Common Vectors
... many others. In standard E. coli strains the copy number of pUC19 can reach well over 100 copies per cell.1 As shown in Table 1, the copy number of this vector is reduced by approximately 25-fold in the CopyCutter EPI400 strain compared to the parental TransforMAX EC100 strain, grown under the same ...
... many others. In standard E. coli strains the copy number of pUC19 can reach well over 100 copies per cell.1 As shown in Table 1, the copy number of this vector is reduced by approximately 25-fold in the CopyCutter EPI400 strain compared to the parental TransforMAX EC100 strain, grown under the same ...
Computers of the Future? Moore`s Law Ending in 2018?
... – Encodes the genetic information of cellular organisms. – Consists of polymer chains, or strands – Each strand may be viewed as a chain of nucleotides, or bases, of length n. – The four DNA nucleotides are adenine, guanine, cytosine and thymine, commonly abbreviated to A,G,C and T respectively. – B ...
... – Encodes the genetic information of cellular organisms. – Consists of polymer chains, or strands – Each strand may be viewed as a chain of nucleotides, or bases, of length n. – The four DNA nucleotides are adenine, guanine, cytosine and thymine, commonly abbreviated to A,G,C and T respectively. – B ...
Slide 1
... • A repeated sequence of 2-5 nucleotides e.g. ACACACACACACACAC = AC8 • Usable repeat lengths are 8-40 copies • Occur in many locations in genome, usually in non-coding regions • Mutation prone (slippage replication) (High mutation rate – 10-2 to 10-5) • Thus any given population may contain variants ...
... • A repeated sequence of 2-5 nucleotides e.g. ACACACACACACACAC = AC8 • Usable repeat lengths are 8-40 copies • Occur in many locations in genome, usually in non-coding regions • Mutation prone (slippage replication) (High mutation rate – 10-2 to 10-5) • Thus any given population may contain variants ...
18.1 Mutations Are Inherited Alterations in the DNA Sequence
... Types of Gene Mutations (based on their molecular nature) • Base substitutions • Transition-Pu for Pu; Py for Py • Transversion-Pu for Py; Py for Pu • Insertions and deletions • Frameshift mutations-disrupts codon pattern • In-frame insertions and deletions-insert or delete number of bases that is ...
... Types of Gene Mutations (based on their molecular nature) • Base substitutions • Transition-Pu for Pu; Py for Py • Transversion-Pu for Py; Py for Pu • Insertions and deletions • Frameshift mutations-disrupts codon pattern • In-frame insertions and deletions-insert or delete number of bases that is ...
Alternative splicing
... After finish the genome sequencing projects, it was realized that only less of the genes had been previously characterized. Two methods are currently used to assign the function of a gene based only on its sequence. ...
... After finish the genome sequencing projects, it was realized that only less of the genes had been previously characterized. Two methods are currently used to assign the function of a gene based only on its sequence. ...
future
... – Encodes the genetic information of cellular organisms. – Consists of polymer chains, or strands – Each strand may be viewed as a chain of nucleotides, or bases, of length n. – The four DNA nucleotides are adenine, guanine, cytosine and thymine, commonly abbreviated to A,G,C and T respectively. – B ...
... – Encodes the genetic information of cellular organisms. – Consists of polymer chains, or strands – Each strand may be viewed as a chain of nucleotides, or bases, of length n. – The four DNA nucleotides are adenine, guanine, cytosine and thymine, commonly abbreviated to A,G,C and T respectively. – B ...
(2) Excision Repair
... • Recent research in bacteria, yeast and mammalian cells • most of the mutations arise by transletion bypass • when highly processive semiconservative DNA replication is arrested at DNA lesions • translesion synthesis (TLS) polymerases allows them to insert nucleotides opposite DNA lesions, but at t ...
... • Recent research in bacteria, yeast and mammalian cells • most of the mutations arise by transletion bypass • when highly processive semiconservative DNA replication is arrested at DNA lesions • translesion synthesis (TLS) polymerases allows them to insert nucleotides opposite DNA lesions, but at t ...
Current Microbiology 40:
... Purification of plasmid DNA, restriction analysis, and gel electrophoresis. For most analytical purposes, plasmids were prepared from overnight cultures by the alkaline lysis method as described [15]. Restriction enzymes were purchased from Boehringer-Mannheim (Montreal, QC) and used with the buffer ...
... Purification of plasmid DNA, restriction analysis, and gel electrophoresis. For most analytical purposes, plasmids were prepared from overnight cultures by the alkaline lysis method as described [15]. Restriction enzymes were purchased from Boehringer-Mannheim (Montreal, QC) and used with the buffer ...
Genomics and Behavior “Central Dogma” Outline
... (group A has greater expression than group B), count the number of transcripts • Can identify splice variants – Some genes like brain derived neurotrophic factor have many splice variants ...
... (group A has greater expression than group B), count the number of transcripts • Can identify splice variants – Some genes like brain derived neurotrophic factor have many splice variants ...
Telomeres - OpenWetWare
... identity between Rpa3 and Ten1, and therefore we cannot conclude whether Ten1 contains an OB-fold domain or not. This may be a reflection of the fact that both proteins have diverged rapidly at the primary sequence level, as revealed by the alignments of Rpa3 and Ten1 sequences from fungal genomes. ...
... identity between Rpa3 and Ten1, and therefore we cannot conclude whether Ten1 contains an OB-fold domain or not. This may be a reflection of the fact that both proteins have diverged rapidly at the primary sequence level, as revealed by the alignments of Rpa3 and Ten1 sequences from fungal genomes. ...
DIFFERENT LEVELS OF PROTEIN STRUCTURE PRIMARY
... In DNA and RNA, the phosphodiester bond is the linkage between the 3' carbon of one sugar and the 5' carbon of the next sugar (i.e. between deoxyribose sugars in DNA and ribose sugars in RNA). It is a group of strong covalent bonds between the phosphorus atom in a phosphate group and two other molec ...
... In DNA and RNA, the phosphodiester bond is the linkage between the 3' carbon of one sugar and the 5' carbon of the next sugar (i.e. between deoxyribose sugars in DNA and ribose sugars in RNA). It is a group of strong covalent bonds between the phosphorus atom in a phosphate group and two other molec ...
BIOL 222 - philipdarrenjones.com
... 3) Where in a DNA molecule can DNA polymerase add new nucleotides? A) 5’ end of new strand B) 3’ end of new strand C) randomly D) alternating 5’ and 3’ ends ...
... 3) Where in a DNA molecule can DNA polymerase add new nucleotides? A) 5’ end of new strand B) 3’ end of new strand C) randomly D) alternating 5’ and 3’ ends ...
SBI 3C genetics Study Guide (SPRING 2015)
... Describe the 3 reasons why cells need to divide Describe the phases of the cell cycle (including mitosis and cytokinesis and the 3 phases of interphase) What is asexual reproduction? Provide examples of organisms that divide through asexual reproduction and compare the DNA in the parent to the DNA i ...
... Describe the 3 reasons why cells need to divide Describe the phases of the cell cycle (including mitosis and cytokinesis and the 3 phases of interphase) What is asexual reproduction? Provide examples of organisms that divide through asexual reproduction and compare the DNA in the parent to the DNA i ...
DNA replication machinery
... action of helicase, which breaks the hydrogen bonds holding the two DNA strands together. The resulting structure has two branching "prongs", each one made up of a single strand of DNA. Leading strand synthesis In DNA replication, the leading strand is defined as the new DNA strand at the replicatio ...
... action of helicase, which breaks the hydrogen bonds holding the two DNA strands together. The resulting structure has two branching "prongs", each one made up of a single strand of DNA. Leading strand synthesis In DNA replication, the leading strand is defined as the new DNA strand at the replicatio ...
Section 8.7 Mutations
... Two Categories of Mutations: 1.Single Gene – affects one gene – usually caused by an error in DNA replication 2. Chromosomal – affects chromosomes – usually error in meiosis . Usually more harmful since many genes are affected. ...
... Two Categories of Mutations: 1.Single Gene – affects one gene – usually caused by an error in DNA replication 2. Chromosomal – affects chromosomes – usually error in meiosis . Usually more harmful since many genes are affected. ...
DNA technologies
... 4. Heat-stable DNA polymerase. Three steps in PCR: 1. Denaturation. Heat to 95°C. Double stranded template DNA denatures (the double stranded DNA helix becomes two separate single stranded templates for PCR). 2. Annealing. Reaction is cooled to temperature below the Annealing temperature of the prim ...
... 4. Heat-stable DNA polymerase. Three steps in PCR: 1. Denaturation. Heat to 95°C. Double stranded template DNA denatures (the double stranded DNA helix becomes two separate single stranded templates for PCR). 2. Annealing. Reaction is cooled to temperature below the Annealing temperature of the prim ...
Bioinformatics - Welcome to the Official Website of
... applying “informatics techniques” (derived from disciplines such as applied maths, computer science ...
... applying “informatics techniques” (derived from disciplines such as applied maths, computer science ...
week 13_genetic information
... cells and is transmitted to offspring, consists of specific sequence of nitrogenous bases. DNA synthesis involves the complementary pairing of nucleotide bases on 2 strands of DNA. Mechanism by which genetic info is decoded and used to direct cellular processes begins with the synthesis of RNA. RNA ...
... cells and is transmitted to offspring, consists of specific sequence of nitrogenous bases. DNA synthesis involves the complementary pairing of nucleotide bases on 2 strands of DNA. Mechanism by which genetic info is decoded and used to direct cellular processes begins with the synthesis of RNA. RNA ...
BIOL 1107 - Chapter 17
... open and denature the DNA, which sticks to the filter at the site of each colony. The filter is incubated with a radioactively labeled probe that can form hybrids with complementary DNA in the gene of interest. ...
... open and denature the DNA, which sticks to the filter at the site of each colony. The filter is incubated with a radioactively labeled probe that can form hybrids with complementary DNA in the gene of interest. ...
A rough guide to molecular biology.
... be physically removed from the chromosomal DNA and amplified. To remove a piece of DNA, restriction enzymes are required. These enzymes are naturally generated in bacteria where they serve a protective function, cutting up foreign DNA at specific sites. Each enzyme, of which hundreds have been ident ...
... be physically removed from the chromosomal DNA and amplified. To remove a piece of DNA, restriction enzymes are required. These enzymes are naturally generated in bacteria where they serve a protective function, cutting up foreign DNA at specific sites. Each enzyme, of which hundreds have been ident ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.