AP Biology Chapter 20 Biotechnology Guided Notes
... Gel Electrophoresis and Southern Blotting • One indirect method of rapidly analyzing and comparing genomes is ___________________ • This technique uses a gel as a _______________ to separate nucleic acids or proteins by ______ ___________________, and other properties • A ________________ is applie ...
... Gel Electrophoresis and Southern Blotting • One indirect method of rapidly analyzing and comparing genomes is ___________________ • This technique uses a gel as a _______________ to separate nucleic acids or proteins by ______ ___________________, and other properties • A ________________ is applie ...
Explain the difference between the following types of genome maps
... copies of the same gene that occur near each other. They are transcribed simultaneously , increasing the amount of mRNA available for protein synthesis. Tandem clusters also include genes that do not encode proteins, such as clusters of rRNA genes. ...
... copies of the same gene that occur near each other. They are transcribed simultaneously , increasing the amount of mRNA available for protein synthesis. Tandem clusters also include genes that do not encode proteins, such as clusters of rRNA genes. ...
protocol: restriction endonuclease digestion/analysis of
... A single PCR-generated band in a gel does not always indicate a single amplification product. One way to quickly determine if there is more than one product is to digest the PCR product with restriction endonucleases. For example, following restriction endonuclease digestion, if DNA fragments are vi ...
... A single PCR-generated band in a gel does not always indicate a single amplification product. One way to quickly determine if there is more than one product is to digest the PCR product with restriction endonucleases. For example, following restriction endonuclease digestion, if DNA fragments are vi ...
Untitled
... the DNase after the treatment. Fast and easy method. The DNase removal step takes place in just 3 minutes. ...
... the DNase after the treatment. Fast and easy method. The DNase removal step takes place in just 3 minutes. ...
Ultrafast Excited-State Dynamics in Nucleic Acids
... electronic energy relaxation greatly reduces the likelihood of photochemical damage. We have recently shown that covalently modified nucleobases [3] and even individual tautomers [4] differ dramatically in their excited-state dynamics. The S1 lifetime of 9-methyladenine is ≈ 200 fs, while the isomer ...
... electronic energy relaxation greatly reduces the likelihood of photochemical damage. We have recently shown that covalently modified nucleobases [3] and even individual tautomers [4] differ dramatically in their excited-state dynamics. The S1 lifetime of 9-methyladenine is ≈ 200 fs, while the isomer ...
Bacterial Conjugation
... the entire bacterial chromosome to the F- cell. The first DNA to be transferred is chromosomal DNA, and the last DNA to be transferred will be the F factor DNA. ...
... the entire bacterial chromosome to the F- cell. The first DNA to be transferred is chromosomal DNA, and the last DNA to be transferred will be the F factor DNA. ...
Chapter 12: DNA & RNA
... DNA – Structure Questions 1.What pair of scientists are largely credited for discovering the shape of the DNA molecule? 2.Name the scientist whose photographs helped solve the mystery of DNA’s structure 3.DNA is in the shape of a _______ _______. 4.What are the sides of the DNA molecule made of? ...
... DNA – Structure Questions 1.What pair of scientists are largely credited for discovering the shape of the DNA molecule? 2.Name the scientist whose photographs helped solve the mystery of DNA’s structure 3.DNA is in the shape of a _______ _______. 4.What are the sides of the DNA molecule made of? ...
Griffith`s Experiment
... 1. RNA polymerase binds to DNA in the nucleus and separates the DNA strand for 1 gene. 2. RNA polymerase “reads” 1 strand of DNA to produce a strand of messenger RNA (mRNA). 3. Complementary RNA nucleotides pair across from the DNA nucleotides (A-U; G-C, C-G; T-A) 4. RNA polymerase links the nucleot ...
... 1. RNA polymerase binds to DNA in the nucleus and separates the DNA strand for 1 gene. 2. RNA polymerase “reads” 1 strand of DNA to produce a strand of messenger RNA (mRNA). 3. Complementary RNA nucleotides pair across from the DNA nucleotides (A-U; G-C, C-G; T-A) 4. RNA polymerase links the nucleot ...
Human Heredity
... repeats that do not code for proteins. This DNA varies from person to person. Here, one sample has 12 repeats between genes A and B, while the second sample has 9 repeats. ...
... repeats that do not code for proteins. This DNA varies from person to person. Here, one sample has 12 repeats between genes A and B, while the second sample has 9 repeats. ...
Forensic-identification
... Let's look at two people and the segments of DNA they carry that contain this RFLP (for clarity, we will only see one of the two stands of DNA). Since Jack and Jill are both diploid organisms, they have two copies of this RFLP. When we examine one copy from Jack and one copy from Jill, we see that ...
... Let's look at two people and the segments of DNA they carry that contain this RFLP (for clarity, we will only see one of the two stands of DNA). Since Jack and Jill are both diploid organisms, they have two copies of this RFLP. When we examine one copy from Jack and one copy from Jill, we see that ...
AP Biology - TeacherWeb
... Transformation = change in phenotype something in heat-killed bacteria could still transmit AP Biology ...
... Transformation = change in phenotype something in heat-killed bacteria could still transmit AP Biology ...
How do I use qPCR to determine the concentration of my material
... Quantitative PCR (qPCR) uses real-time fluorescence to measure the quantity of DNA present at each cycle during a PCR. A wide variety of approaches have been developed for generating a fluorescent signal, the most common of which use either hydrolysis probes (e.g., TaqMan®), or a double-stranded DNA ...
... Quantitative PCR (qPCR) uses real-time fluorescence to measure the quantity of DNA present at each cycle during a PCR. A wide variety of approaches have been developed for generating a fluorescent signal, the most common of which use either hydrolysis probes (e.g., TaqMan®), or a double-stranded DNA ...
DNA Replication - Gadjah Mada University
... Watson and Crick the two strands of the parental molecule separate, and each functions as a template for synthesis of a new complementary strand. DNA Template ...
... Watson and Crick the two strands of the parental molecule separate, and each functions as a template for synthesis of a new complementary strand. DNA Template ...
Heterologous Protein Production in Eukaryotic Cells
... • When cells are grown on glucose, transcription from the GAL4 promoter is down-regulated, there is less Gal4p in the cells and reduced GAL4 gene under the GAL1 promoter control level of activator binding. The protein production is initiated by switching the cells into a galactose-containing medium. ...
... • When cells are grown on glucose, transcription from the GAL4 promoter is down-regulated, there is less Gal4p in the cells and reduced GAL4 gene under the GAL1 promoter control level of activator binding. The protein production is initiated by switching the cells into a galactose-containing medium. ...
The relationship between genes and traits is often complex
... (only if needed) Bonus #2 posted Year End Topics: •mtDNA •Mapping •Probability •Evolution and the Origin of Humans ...
... (only if needed) Bonus #2 posted Year End Topics: •mtDNA •Mapping •Probability •Evolution and the Origin of Humans ...
Rapid Communication: Mapping of the Titin (TTN) Gene to Pig
... Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragmen ...
... Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragmen ...
Taq Polymerase - cloudfront.net
... Taq polymerase can withstand temperatures needed to produce the best effects in this machine, so scientists can run many PCR cycles automatically. PCR involves denaturing, annealing and replication steps, usually repeated 20 to 30 times. Denaturing separates the double-stranded DNA into single stran ...
... Taq polymerase can withstand temperatures needed to produce the best effects in this machine, so scientists can run many PCR cycles automatically. PCR involves denaturing, annealing and replication steps, usually repeated 20 to 30 times. Denaturing separates the double-stranded DNA into single stran ...
DISCOVERING DNA Biology Practical—DNA extraction
... regulatory sequences, introns and ‘junk’ DNA. Some of these noncoding sequences may have as yet undiscovered functions. In addition to genomic DNA, mitochondria contain their own DNA which replicates independently from cell chromosomal DNA. All plant and animal cells contain DNA, but there are some ...
... regulatory sequences, introns and ‘junk’ DNA. Some of these noncoding sequences may have as yet undiscovered functions. In addition to genomic DNA, mitochondria contain their own DNA which replicates independently from cell chromosomal DNA. All plant and animal cells contain DNA, but there are some ...
Datasheet - BioVision
... Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines for amplification reactions Isolation of mRNA or genomic DNA from different tissues including mouse tail or from cultured cells For modifying glycoprotein for structural studies To treat tissue sections for in situ hy ...
... Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines for amplification reactions Isolation of mRNA or genomic DNA from different tissues including mouse tail or from cultured cells For modifying glycoprotein for structural studies To treat tissue sections for in situ hy ...
Constructing and Screening a Recombinant DNA Library
... Bacterial AND yeast origin of replications, a selection marker in bacteria, multiple cloning site/restriction enzyme site. A yeast promoter is not required because we are looking at genomic DNA. d) You digest both the yeast genomic DNA and many copies of the vector with the BamH1 restriction enzyme. ...
... Bacterial AND yeast origin of replications, a selection marker in bacteria, multiple cloning site/restriction enzyme site. A yeast promoter is not required because we are looking at genomic DNA. d) You digest both the yeast genomic DNA and many copies of the vector with the BamH1 restriction enzyme. ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.