Antibiotic resistance genes are carried on plasmids
... The main differences among commercially available plasmids are the number of restriction enzyme sites, their order in the multiple cloning site, the type of antibiotic resistance that the plasmid confers, and some other genetic information that makes the plasmid useful for a specific purpose. Bacter ...
... The main differences among commercially available plasmids are the number of restriction enzyme sites, their order in the multiple cloning site, the type of antibiotic resistance that the plasmid confers, and some other genetic information that makes the plasmid useful for a specific purpose. Bacter ...
File
... chemicals, known as restriction enzymes, to cut DNA into fragments that can later be incorporated into another DNA strand. • Restriction enzymes can be thought of as highly specialized scissors that cut a DNA molecule when it recognizes a specific sequence of bases. • Once a portion of the DNA stran ...
... chemicals, known as restriction enzymes, to cut DNA into fragments that can later be incorporated into another DNA strand. • Restriction enzymes can be thought of as highly specialized scissors that cut a DNA molecule when it recognizes a specific sequence of bases. • Once a portion of the DNA stran ...
DNA replication - U of L Class Index
... yeast, and SV40 are very different, they share several properties: Replication origins are unique DNA segments that contain multiple short repeated sequences. These short repeat units are recognized by multimeric originbinding proteins. These proteins play a key role in assembling DNA polymerases ...
... yeast, and SV40 are very different, they share several properties: Replication origins are unique DNA segments that contain multiple short repeated sequences. These short repeat units are recognized by multimeric originbinding proteins. These proteins play a key role in assembling DNA polymerases ...
Supplementary Text 1 (doc 52K)
... sequences not included in the MMC showed no mismatch. As the first primer set covers more sequences of the MMC than the second, the first system was chosen for screening of environmental samples. To determine the optimal annaeling temperatures for PCR and to avoid unspecific amplification, DNA from ...
... sequences not included in the MMC showed no mismatch. As the first primer set covers more sequences of the MMC than the second, the first system was chosen for screening of environmental samples. To determine the optimal annaeling temperatures for PCR and to avoid unspecific amplification, DNA from ...
On Limits of Performance of DNA Microarrays
... the probabilistic and quantum mechanical nature of molecular interactions present in these system [3]. Finally, the fluorescent labels in each spot are measured to obtain an image, having correlation to the hybridization process, and thus the gene expression levels. Today, the sensitivity, dynamic ra ...
... the probabilistic and quantum mechanical nature of molecular interactions present in these system [3]. Finally, the fluorescent labels in each spot are measured to obtain an image, having correlation to the hybridization process, and thus the gene expression levels. Today, the sensitivity, dynamic ra ...
L.R. Huang, J.O. Tegenfeldt, J.J. Kraeft, J.C. Sturm, R.H. Austin, E.C. Cox, "Generation of large-area tunable uniform electric fields in microfluid arrays for rapid DNA separation," Tech. Dig. Int. Elect. Dev. Mtg., pp. 363-366 (2001).
... molecules in microfluidic systems. In this paper we present a novel method for generating tunable uniform electric fields over large microfluidic arrays in two dimensions, and its application to a microfabricated device that separates genomic DNA. The device fractionates large DNA molecules over thr ...
... molecules in microfluidic systems. In this paper we present a novel method for generating tunable uniform electric fields over large microfluidic arrays in two dimensions, and its application to a microfabricated device that separates genomic DNA. The device fractionates large DNA molecules over thr ...
Document
... Genetic information written in codons is translated into amino acid sequences • The “words” of the DNA “language” are triplets of bases called codons – 3 bases or nucleotides make one codon – Each codon specifies an amino acid – The codons in a gene specify the amino acid sequence of a polypeptide ...
... Genetic information written in codons is translated into amino acid sequences • The “words” of the DNA “language” are triplets of bases called codons – 3 bases or nucleotides make one codon – Each codon specifies an amino acid – The codons in a gene specify the amino acid sequence of a polypeptide ...
L 04 _transcription
... messenger RNA. DNA is in the nucleus, but protein synthesis occurs in the cytoplasm. The DNA sequence of a gene is copied into an RNA sequence by transcription; the RNA copy of a gene is the mRNA. About 2-3% of the total RNA in a cell. transfer RNA. There is no chemical basis for amino acids to reco ...
... messenger RNA. DNA is in the nucleus, but protein synthesis occurs in the cytoplasm. The DNA sequence of a gene is copied into an RNA sequence by transcription; the RNA copy of a gene is the mRNA. About 2-3% of the total RNA in a cell. transfer RNA. There is no chemical basis for amino acids to reco ...
THINK ABOUT IT
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
... Molecular biology seeks to explain living organisms by studying them at the molecular level, using molecules like DNA and RNA. The central dogma of molecular biology is that information is transferred from DNA to RNA to protein. ...
Lecture 7 - School of Science and Technology
... number of sequences covering the whole genome also varies very significantly 10 – 106. • DNA fragments presented in DB have not only very different lengths but also diverse origin. Some are large fragments of genome, other represent genes or their fragments, some are repeats and noncoding sequences, ...
... number of sequences covering the whole genome also varies very significantly 10 – 106. • DNA fragments presented in DB have not only very different lengths but also diverse origin. Some are large fragments of genome, other represent genes or their fragments, some are repeats and noncoding sequences, ...
File - Year 11 Science
... (i) As a result of genetic modification, these tomatoes produce a new substance which has health benefits and turns the tomatoes purple. Name this substance. ...
... (i) As a result of genetic modification, these tomatoes produce a new substance which has health benefits and turns the tomatoes purple. Name this substance. ...
DNA Testing of Seafood: Technical Guide
... There are many scientific uses for these techniques. For example Bui et al [11] used DNA methods to determine the species of eggs in plankton samples, in order to map spawning grounds of cod off eastern Canada. Fish stocks are assessed in given geographical areas described as ‘management stocks’. ...
... There are many scientific uses for these techniques. For example Bui et al [11] used DNA methods to determine the species of eggs in plankton samples, in order to map spawning grounds of cod off eastern Canada. Fish stocks are assessed in given geographical areas described as ‘management stocks’. ...
Simulating Protein Synthesis to create a CHNOPS! Read the
... mRNA leaves the nucleus and travels to the ribosome where proteins are made. The 3-base codons in the mRNA strand will pair up with anticodons on the transfer RNA (tRNA) molecules. Each tRNA carries an amino acid to the ribosome, and these amino acids link together to form a protein. The process i ...
... mRNA leaves the nucleus and travels to the ribosome where proteins are made. The 3-base codons in the mRNA strand will pair up with anticodons on the transfer RNA (tRNA) molecules. Each tRNA carries an amino acid to the ribosome, and these amino acids link together to form a protein. The process i ...
Multiple PCR analyses on trace amounts of DNA
... were applied to increase yield of the products. Sequencing and SSCP analysis of the specific PCR products confirmed the high fidelity of the RP-PCR. In titration tests of high molecular weight template DNA RP-PCR increased the sensitivity of specific PCR by over 100 times. Amplification of DNA fragm ...
... were applied to increase yield of the products. Sequencing and SSCP analysis of the specific PCR products confirmed the high fidelity of the RP-PCR. In titration tests of high molecular weight template DNA RP-PCR increased the sensitivity of specific PCR by over 100 times. Amplification of DNA fragm ...
Questions
... one with two new strands and the other with two original each with one new strand and one original strand each with two original strands 6. The backbone of a DNA molecule is made up of alternating _______ and _____ groups. Hide answers nitrogen bases, phosphate enzymes, nitrogen deoxyribose sugar, p ...
... one with two new strands and the other with two original each with one new strand and one original strand each with two original strands 6. The backbone of a DNA molecule is made up of alternating _______ and _____ groups. Hide answers nitrogen bases, phosphate enzymes, nitrogen deoxyribose sugar, p ...
5 min Insect DNA/RNA Preservation and Extraction Kit
... Biofactories’ 5 min Insect DNA/RNA Preservation and Extraction Kit provides the fastest method for the storage/preservation and isolation/purification of total DNA/RNA from insect samples. The kit is specially designed for preservation and extraction of cellular and viral DNA/RNA from insect such as ...
... Biofactories’ 5 min Insect DNA/RNA Preservation and Extraction Kit provides the fastest method for the storage/preservation and isolation/purification of total DNA/RNA from insect samples. The kit is specially designed for preservation and extraction of cellular and viral DNA/RNA from insect such as ...
2011
... It produces 3 times as much because each molecule of pyruvate produces three (3) molecules of CO2 as it moves into and through the Krebs cycle. +4 points ...
... It produces 3 times as much because each molecule of pyruvate produces three (3) molecules of CO2 as it moves into and through the Krebs cycle. +4 points ...
Karyotyping, FISH and CGH array
... In principle, both karyotyping and arrays are genome-wide technologies which can be used to assess the presence of genomic imbalance such as copy number variations (CNVs). Although they may look like very different technologies, the primary difference between them is in the resolution, which is a me ...
... In principle, both karyotyping and arrays are genome-wide technologies which can be used to assess the presence of genomic imbalance such as copy number variations (CNVs). Although they may look like very different technologies, the primary difference between them is in the resolution, which is a me ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.