Table S4.
... 11. Gimeno CJ, Ljungdahl PO, Styles CA, Fink GR (1992) Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell 68: 1077-1090. 12. Herrero AB, Lopez MC, Fernandez-Lago L, Dominguez A (1999) Candida albicans and Yarrowia lipolytica as alter ...
... 11. Gimeno CJ, Ljungdahl PO, Styles CA, Fink GR (1992) Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell 68: 1077-1090. 12. Herrero AB, Lopez MC, Fernandez-Lago L, Dominguez A (1999) Candida albicans and Yarrowia lipolytica as alter ...
Higher Biology Course Assessment Specification
... cells by reference to the repair of damaged or diseased organs or tissues. Stem cell research provides information on how cell processes such as cell growth, differentiation and gene regulation work. Stem cells can be used as model cells to study how diseases develop or for drug testing. The ethical ...
... cells by reference to the repair of damaged or diseased organs or tissues. Stem cell research provides information on how cell processes such as cell growth, differentiation and gene regulation work. Stem cells can be used as model cells to study how diseases develop or for drug testing. The ethical ...
ch4-TheGenomicBiologistsToolKit_1.3
... recombinant DNA. Molecular cloning methods are central to many areas of biology, biotechnology, and medicine, including DNA sequencing. The DNA from host organism in a cloning experiment, often called a vector, typically has 3 things: 1) Sequences necessary to produce recombinant DNA and facilitate ...
... recombinant DNA. Molecular cloning methods are central to many areas of biology, biotechnology, and medicine, including DNA sequencing. The DNA from host organism in a cloning experiment, often called a vector, typically has 3 things: 1) Sequences necessary to produce recombinant DNA and facilitate ...
CH4. The Genomic Biologists Toolkit
... recombinant DNA. Molecular cloning methods are central to many areas of biology, biotechnology, and medicine, including DNA sequencing. The DNA from host organism in a cloning experiment, often called a vector, typically has 3 things: 1) Sequences necessary to produce recombinant DNA and facilitate ...
... recombinant DNA. Molecular cloning methods are central to many areas of biology, biotechnology, and medicine, including DNA sequencing. The DNA from host organism in a cloning experiment, often called a vector, typically has 3 things: 1) Sequences necessary to produce recombinant DNA and facilitate ...
Guanine-Plus-Cytosine Content of Rothia dentocaviosa
... figures, 47 to 53 mol% G + C , for five strains and cited unpublished data from Hammond. These data were cited by Schaal and Pulverer (9). To resolve this discrepancy, we determined the G+C contents of two American Type Culture Collection strains of R. dentocariosa and one clinical isolate. Strains ...
... figures, 47 to 53 mol% G + C , for five strains and cited unpublished data from Hammond. These data were cited by Schaal and Pulverer (9). To resolve this discrepancy, we determined the G+C contents of two American Type Culture Collection strains of R. dentocariosa and one clinical isolate. Strains ...
Glencoe Biology
... A person wishes to raise guinea pigs with black fur, the dominant trait. She selects a male black guinea pig and performs a test cross with a female that has white fur, the recessive trait. What is the black guinea pig’s genotype if any of the offspring are white? ...
... A person wishes to raise guinea pigs with black fur, the dominant trait. She selects a male black guinea pig and performs a test cross with a female that has white fur, the recessive trait. What is the black guinea pig’s genotype if any of the offspring are white? ...
Replication of a DNA Microarray
... replicas are exposed to fluorescently labeled, noncomplementary ssDNA. Third, the amount of DNA transferred decreases with each successive array replication. Specifically, the net fluorescence intensities arising from the first, second, and third replicas are respectively 16, 14, and 11 counts/s. Th ...
... replicas are exposed to fluorescently labeled, noncomplementary ssDNA. Third, the amount of DNA transferred decreases with each successive array replication. Specifically, the net fluorescence intensities arising from the first, second, and third replicas are respectively 16, 14, and 11 counts/s. Th ...
Factors affecting the amount of genomic DNA
... It can be imagined that two main factors affect the amount of ‘donor’ DNA that can be extracted from faeces. The first is the amount of DNA initially present in the sample and the second is the amount still present after collection and storage. Until now, research focusing on the former topic was li ...
... It can be imagined that two main factors affect the amount of ‘donor’ DNA that can be extracted from faeces. The first is the amount of DNA initially present in the sample and the second is the amount still present after collection and storage. Until now, research focusing on the former topic was li ...
PDF - 167.13 kbytes - Istituto Superiore di Sanità
... The LAMP is a molecular biology technique that allows the amplification of specific nucleic acid fragments, which initial and terminal nucleotide sequences are known (oligonucleotide pair). If a species has its own characteristic DNA portion, due to its composition, it is possible to design 2 or 3 p ...
... The LAMP is a molecular biology technique that allows the amplification of specific nucleic acid fragments, which initial and terminal nucleotide sequences are known (oligonucleotide pair). If a species has its own characteristic DNA portion, due to its composition, it is possible to design 2 or 3 p ...
Recombinant DNA Technology
... dissociates into two single strands. This process, called DNA denaturation, was for many years thought to be irreversible. In 1961, however, it was discovered that complementary single strands of DNA will readily re-form double helices (a process called DNA renaturation or hybridization) if they are ...
... dissociates into two single strands. This process, called DNA denaturation, was for many years thought to be irreversible. In 1961, however, it was discovered that complementary single strands of DNA will readily re-form double helices (a process called DNA renaturation or hybridization) if they are ...
Platinum DNA polymerases
... Fidelity compared to Taq Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq ...
... Fidelity compared to Taq Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq ...
A BB B BB - AIMS Press
... starting amount of material is at a very low concentration. qPCR increased the rapidity of the detection in comparison to the agarose gel electrophoresis by the inclusion of fluorescent reporter molecules that enable the quantification of PCR products in real time by monitoring the amplification of ...
... starting amount of material is at a very low concentration. qPCR increased the rapidity of the detection in comparison to the agarose gel electrophoresis by the inclusion of fluorescent reporter molecules that enable the quantification of PCR products in real time by monitoring the amplification of ...
used without further purification. Methicillin
... plate and then incubated for 24 hours at 370C. At the end, a single colony from tryptic agar plate was inoculated into 10 ml of Tryptic Soy Broth and then it was incubated at 37oC for 16 hours in a shaker at 150 rpm which has an inoculum of 108 CFU/ml. All the growth medium and agar were autoclaved ...
... plate and then incubated for 24 hours at 370C. At the end, a single colony from tryptic agar plate was inoculated into 10 ml of Tryptic Soy Broth and then it was incubated at 37oC for 16 hours in a shaker at 150 rpm which has an inoculum of 108 CFU/ml. All the growth medium and agar were autoclaved ...
Preparation of MyoD mRNA for the differentiation of stem cells into
... found that transfecting the cell with mRNAs is the better transfection method, since mRNAs are the templates for translation to make proteins and there is little concern for their possible integration into the genome, which would be a complication of the DNA transfection method. By transfecting the ...
... found that transfecting the cell with mRNAs is the better transfection method, since mRNAs are the templates for translation to make proteins and there is little concern for their possible integration into the genome, which would be a complication of the DNA transfection method. By transfecting the ...
United States District Court, D. Delaware UNITED STATES OF
... genetic code. A gene is a particular DNA sequence located along a chromosome, ranging from a few thousand to tens of thousands of base pairs, that produces a specific product in the body. In other words, a gene is a site (a sequence of letters) on the DNA that encodes for a protein. A marker is a si ...
... genetic code. A gene is a particular DNA sequence located along a chromosome, ranging from a few thousand to tens of thousands of base pairs, that produces a specific product in the body. In other words, a gene is a site (a sequence of letters) on the DNA that encodes for a protein. A marker is a si ...
Microbiology 201
... 2. It can be grown in pure culture. {Problem: viruses & intracellular parasitic bacteria can't be grown outside living cells} 3. A lab animal can be infected with the agent and it suffers the disease. {Problem: host species specificity} 4. The microbe can be reisolated from the lab animal. Potato sl ...
... 2. It can be grown in pure culture. {Problem: viruses & intracellular parasitic bacteria can't be grown outside living cells} 3. A lab animal can be infected with the agent and it suffers the disease. {Problem: host species specificity} 4. The microbe can be reisolated from the lab animal. Potato sl ...
Microbiology 201
... 2. It can be grown in pure culture. {Problem: viruses & intracellular parasitic bacteria can't be grown outside living cells} 3. A lab animal can be infected with the agent and it suffers the disease. {Problem: host species specificity} 4. The microbe can be reisolated from the lab animal. Potato sl ...
... 2. It can be grown in pure culture. {Problem: viruses & intracellular parasitic bacteria can't be grown outside living cells} 3. A lab animal can be infected with the agent and it suffers the disease. {Problem: host species specificity} 4. The microbe can be reisolated from the lab animal. Potato sl ...
16_Lecture_Presentation
... various proteins within the white blood cells. • he came across a substance from the cell nuclei that had chemical properties unlike any protein, – -a much higher phosphorous ...
... various proteins within the white blood cells. • he came across a substance from the cell nuclei that had chemical properties unlike any protein, – -a much higher phosphorous ...
Comparative studies on molecular techniques for detecting
... Sensitivity of PCR, hybridization and PCR-hybridization to detect R. tsutsugamushi Seven serial dilutes of Karp strain DNA were detected by the above three methods. It was demonstrated that the highest diluted concentration which could be detected by PCR was 1:105 (Fig. 1), by hybridization was 1:1 ...
... Sensitivity of PCR, hybridization and PCR-hybridization to detect R. tsutsugamushi Seven serial dilutes of Karp strain DNA were detected by the above three methods. It was demonstrated that the highest diluted concentration which could be detected by PCR was 1:105 (Fig. 1), by hybridization was 1:1 ...
Cloning, Expression, and Nucleotide Sequence of lid?
... strain AE79 (ZivR, leu). This strain shows a derepressed rate of leucine transport in the presence of leucine (open bar) which was significantly reduced when the plasmid PANT1 was present (stippled bar). To determine whether the inserted fragment of the plasmid PANT1 also complemented the lstR mutat ...
... strain AE79 (ZivR, leu). This strain shows a derepressed rate of leucine transport in the presence of leucine (open bar) which was significantly reduced when the plasmid PANT1 was present (stippled bar). To determine whether the inserted fragment of the plasmid PANT1 also complemented the lstR mutat ...
Protocol for RiboShredder™ RNase Blend
... of applications. When used for DNA purification, all unwanted RNA can be removed using a simple 10-minute procedure. After the reaction is complete, RiboShredder RNase Blend can be removed using a phenol-chloroform extraction procedure. It’s broad range of salt tolerance makes it ideal for use in ma ...
... of applications. When used for DNA purification, all unwanted RNA can be removed using a simple 10-minute procedure. After the reaction is complete, RiboShredder RNase Blend can be removed using a phenol-chloroform extraction procedure. It’s broad range of salt tolerance makes it ideal for use in ma ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".