Genetic Engineering
... – The use of microorganisms to make cheese and wine – Selective breeding of livestock and crops – Production of antibiotics from ...
... – The use of microorganisms to make cheese and wine – Selective breeding of livestock and crops – Production of antibiotics from ...
Plasmid
... Plasmid can be used for gene transfer into human cells so that it may express the protein that is lacking in the cells. ...
... Plasmid can be used for gene transfer into human cells so that it may express the protein that is lacking in the cells. ...
Additional Slides Ch Biotech Dr Violet
... the disease is actually one and the same as the mutation that gives rise to the polymorphism. Direct detection by RFLPs of diseases that result from point mutations is at present limited to only a few genetic diseases. • Sickle cell anemia is caused by a point mutation. The sequence altered by the m ...
... the disease is actually one and the same as the mutation that gives rise to the polymorphism. Direct detection by RFLPs of diseases that result from point mutations is at present limited to only a few genetic diseases. • Sickle cell anemia is caused by a point mutation. The sequence altered by the m ...
Pre – AP Biology
... – The machine uses heat, DNA Primers, enzymes and a constant supply of nucleosides to build DNA molecules that are identical to the original molecule in nucleotide sequence. – First step: Heat is used to separate the DNA double helix so that replication can occur. – Second step: The attachment of a ...
... – The machine uses heat, DNA Primers, enzymes and a constant supply of nucleosides to build DNA molecules that are identical to the original molecule in nucleotide sequence. – First step: Heat is used to separate the DNA double helix so that replication can occur. – Second step: The attachment of a ...
Study Guide MBMB 451A Fall 2002
... 9. How do you determine the L, T, and W values for a closed circular DNA? 10. Define DNA supercoiling and topoisomerases. 11. How does writhe changes when DNA is wrapped around protein in either a left-handed or right-handed direction? 12. What are DNase hypersensitive sites and what is their relati ...
... 9. How do you determine the L, T, and W values for a closed circular DNA? 10. Define DNA supercoiling and topoisomerases. 11. How does writhe changes when DNA is wrapped around protein in either a left-handed or right-handed direction? 12. What are DNase hypersensitive sites and what is their relati ...
Strawberry DNA extraction:
... Today we will isolate DNA from strawberry cell. Ripe fruit is a good material for isolating plant DNA since the cell walls are already weakened by the ripening process. Commercial strawberries 8 sets of each type of chromosome (this is called octoploid). Wild strawberries have only 2 sets of chromos ...
... Today we will isolate DNA from strawberry cell. Ripe fruit is a good material for isolating plant DNA since the cell walls are already weakened by the ripening process. Commercial strawberries 8 sets of each type of chromosome (this is called octoploid). Wild strawberries have only 2 sets of chromos ...
cloning
... (2) The antibodies can be make by injecting the protein into an animal, and collecting the antibodies that animal made to the protein b) This method only works with expression libraries, since these actually produce the gene product (protein) c) Monoclonal antibodies (1) These are antibodies made fr ...
... (2) The antibodies can be make by injecting the protein into an animal, and collecting the antibodies that animal made to the protein b) This method only works with expression libraries, since these actually produce the gene product (protein) c) Monoclonal antibodies (1) These are antibodies made fr ...
Southern Blotting
... • Polymorphism: is a genetic variant that appears in at least 1% of a population • Such variations include: ABO blood type, Rhesus factor, and major histocompatibility complex (MHC). • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different len ...
... • Polymorphism: is a genetic variant that appears in at least 1% of a population • Such variations include: ABO blood type, Rhesus factor, and major histocompatibility complex (MHC). • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different len ...
Genetics - CBSD.org
... General Definitions • Allele alternate form of a gene • Complete dominance one allele completely hides the other • Incomplete dominance both alleles influence the phenotype (blending) • Codominance Neither allele completely hides the other (both are seen) (blood typing & spots) • Trait an expr ...
... General Definitions • Allele alternate form of a gene • Complete dominance one allele completely hides the other • Incomplete dominance both alleles influence the phenotype (blending) • Codominance Neither allele completely hides the other (both are seen) (blood typing & spots) • Trait an expr ...
transformation
... DNA Transformation is a common technique used in genetic engineering. It involves inserting a target sequence of DNA into an organism that didn’t originally contain that target sequence. Very often, the target sequence is a gene of interest and the organism is a bacterium. Once the gene is inserted ...
... DNA Transformation is a common technique used in genetic engineering. It involves inserting a target sequence of DNA into an organism that didn’t originally contain that target sequence. Very often, the target sequence is a gene of interest and the organism is a bacterium. Once the gene is inserted ...
Sophomore Dental and Optometry Microbiology
... – Thermophiles - >50oC – Psychrophiles - 4oC to 20oC – Mesophiles - 20oC to 40oC ...
... – Thermophiles - >50oC – Psychrophiles - 4oC to 20oC – Mesophiles - 20oC to 40oC ...
Exam 1 - Faculty Web Pages
... 8. By using maps to localize the source of a cholera epidemic in London to a specific public water pump, he was able to reduce its spread by implementing public health measures. 9. He developed a vaccine for rabies. 10. Using colony isolation techniques on agar media, he isolated the anthrax and tub ...
... 8. By using maps to localize the source of a cholera epidemic in London to a specific public water pump, he was able to reduce its spread by implementing public health measures. 9. He developed a vaccine for rabies. 10. Using colony isolation techniques on agar media, he isolated the anthrax and tub ...
DNA Isolation from small tissue samples using salt and spermine
... washing the remaining DNA pellet with 90% ethanol, dissolve in 200 ixl TE buffer, and add 0.1 ml of 5 M ammonium acetate and 0.4 ml of cold absolute ethanol. The DNA is allowed to precipitate either at -20°C overnight or at -80°C for 30 min. Centrifuge the samples for 15 minutes at 4°C at 17,000 g a ...
... washing the remaining DNA pellet with 90% ethanol, dissolve in 200 ixl TE buffer, and add 0.1 ml of 5 M ammonium acetate and 0.4 ml of cold absolute ethanol. The DNA is allowed to precipitate either at -20°C overnight or at -80°C for 30 min. Centrifuge the samples for 15 minutes at 4°C at 17,000 g a ...
AP genetic technology
... • DNA from areas with tandem repeats is cut with restriction enzymes • Because of the variation in the amount of repeated DNA, the restriction fragments vary in size • Variation is detected by gel electrophoresis ...
... • DNA from areas with tandem repeats is cut with restriction enzymes • Because of the variation in the amount of repeated DNA, the restriction fragments vary in size • Variation is detected by gel electrophoresis ...
Gene targeting (contd)
... – not readily possible to synthesize Electroporation - electricity driven transfection • principle is that brief, strong electrical pulse creates transient pores in the cell membrane that allows exchange of molecules • cells and DNA are placed into a cuvette between two plates. – High DC voltage(500 ...
... – not readily possible to synthesize Electroporation - electricity driven transfection • principle is that brief, strong electrical pulse creates transient pores in the cell membrane that allows exchange of molecules • cells and DNA are placed into a cuvette between two plates. – High DC voltage(500 ...
EDVOTEK 225 DNA Fingerprinting
... • Restriction enzymes are endonucleases that catalyze cleavage of phosphate bonds • Require Mg-2 for activity • Generate 5’ phosphae and 3’ hydroxyl group • Endonuclease claves at specific sequence of bases. • Produce by bacteria ...
... • Restriction enzymes are endonucleases that catalyze cleavage of phosphate bonds • Require Mg-2 for activity • Generate 5’ phosphae and 3’ hydroxyl group • Endonuclease claves at specific sequence of bases. • Produce by bacteria ...
DNA gel electrophoresis
... 3-Sample combs: Are used to form sample wells in the gel. 4-Electrophoresis buffer, usually Tris-acetate-EDTA (TAE) or Tris-borate-EDTA (TBE). 5-Loading buffer, which contains something dense (e.g. glycerol or sucrose) to allow the sample to "fall" into the sample wells, and one or two tracking dyes ...
... 3-Sample combs: Are used to form sample wells in the gel. 4-Electrophoresis buffer, usually Tris-acetate-EDTA (TAE) or Tris-borate-EDTA (TBE). 5-Loading buffer, which contains something dense (e.g. glycerol or sucrose) to allow the sample to "fall" into the sample wells, and one or two tracking dyes ...
BIO-RAD Lambda DNA Kit, AP Bio Lab 6B, and BIO
... • Prepare electrophoresis TAE buffer • 11/13 at before 5th period Pour 8 gels (1%, EtBr, 10 well comb) • Prep. HindIII standard (right before class) ...
... • Prepare electrophoresis TAE buffer • 11/13 at before 5th period Pour 8 gels (1%, EtBr, 10 well comb) • Prep. HindIII standard (right before class) ...
Marine Bacteria and Archaea
... C. The new copy of DNA is attached at a membrane site near the parent DNA molecule. ...
... C. The new copy of DNA is attached at a membrane site near the parent DNA molecule. ...
Ch11 Answers to Concept Check Questions
... Concept check: Explain why the mouse in part (d) died. Answer: In this experiment, the type R bacteria had taken up genetic material from the heat-killed type S bacteria, which converted the type R bacteria into type S. This enabled them to proliferate within the mouse and kill it. FIGURE 11.2 Conce ...
... Concept check: Explain why the mouse in part (d) died. Answer: In this experiment, the type R bacteria had taken up genetic material from the heat-killed type S bacteria, which converted the type R bacteria into type S. This enabled them to proliferate within the mouse and kill it. FIGURE 11.2 Conce ...
Pre-lab 1 and Lab 1 2010 - Sonoma Valley High School
... The core of the genetic engineering - carefully planned changes to DNA lead to production of specific proteins Genetic disease can be treated using proteins produced by bacteria whose DNA has been changed by the addition of the corresponding human gene. Those who carry out genetic engineering use ve ...
... The core of the genetic engineering - carefully planned changes to DNA lead to production of specific proteins Genetic disease can be treated using proteins produced by bacteria whose DNA has been changed by the addition of the corresponding human gene. Those who carry out genetic engineering use ve ...
Recombinant DNA - Fulton County Schools
... – Transgenic organisms can be produced by the insertion of recombinant DNA into the genome of a host organism. – Like bacterial plasmids, the DNA molecules used for transformation of plant and animal cells contain genetic markers that help scientists identify which cells have been transformed. ...
... – Transgenic organisms can be produced by the insertion of recombinant DNA into the genome of a host organism. – Like bacterial plasmids, the DNA molecules used for transformation of plant and animal cells contain genetic markers that help scientists identify which cells have been transformed. ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".