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DNA sequencing by the Sanger method
DNA sequencing by the Sanger method

... DNA sequencing by the Sanger method The standard DNA sequencing technique is the Sanger method, named for its developer, Frederick Sanger, who shared the 1980 Nobel Prize in Chemistry. This method begins with the use of special enzymes to synthesize fragments of DNA that terminate when a selected ba ...
Genetics: Chapter 7
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... • Bacterial cells may also contain small circular chromosomes called plasmids (4kb - 100kb; 1 - 1000 copies) that code for optional functions such as antibiotic resistance • Will look at circular DNA in this lecture • The bacterial chromosome is 1000 times longer than the cell - it is not tangled up ...
Chapter 7_microbialgeneticspart1_7e
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... • The science of heredity; includes the study of genes, how they carry information, how they are replicated, how they are expressed ...
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DNA, RNA, Genes, Chromosomes

... Genes are the biological units of heredity. They determine obvious traits, such as hair and eye color, as well as more subtle characteristics, such as the oxygencarrying ability of the blood. Complex traits, such as IQ and physical strength, may be shaped by the interaction of a number of different ...
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Biotechnology Part 3 Outline
Biotechnology Part 3 Outline

... Bacterial Cloning Process A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These en ...
6 Day 9 Biotechnology Part 3 Outline
6 Day 9 Biotechnology Part 3 Outline

... Bacterial Cloning Process A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These en ...
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... has allowed the use or small and degraded DNA samples which has been a major breakthrough  especially within the field of Forensics. Even though PCR/STR is the most common technique used today  Y‐STR and mitochondrial DNA typing are new technologies that can often prove to be very valuable  when dea ...
Chapter 15 - jl041.k12.sd.us
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... DNA molecule is not bound up with histones. Thus, gene regulation in prokaryotes is unique. One of the best known pathways of gene recognition is the lac Operon, a regulatory pathway by which bacteria are able to produce the enzyme to digest lactose only when necessary (when lactose is present in th ...
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... fecal contamination of surface drinking water sources, it is very difficult to distinguish between contamination from human versus animal sources. Presently used methods of microbial source tracking (MST) such as antibiotic resistance, phenotype number ratios, DNA sequencing and ribotyping all have ...
Genetic Engineering
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... DNA cloning One approach is to isolate the gene(s) responsible for the expression of a protein or the formation of a product. The solution to this dilemma is to place a relatively short fragment of a genome, which might contain the gene or other sequence of interest, in an autonomously replicating p ...
CONFOUNDING PHYLOGENETIC TREES
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... -according to rRNA based phylogenies, there are 3 kingdoms of life – bacteria, archaea and eukarya with eukarya derived from archaea -the sequencing of hundreds of genomes has called into question this tree because many proteins in any one organism can be archaeal or bacterial in origin -gene swappi ...
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... • Most eukaryotic introns have “canonical splice sites,” GT---AG (mRNA: GU---AG). • Gene prediction programs search for patterns to predict genes and their structure. • Different gene prediction programs may predict different genes and/or structures. ...
Chapter 9, part A
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Biology Ch. 12 Vocab
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Biology 105: Biology Science for Life with Physiology, 3rd Ed., Belk
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... recognized as safe (GRAS); 20 genetically modified organism (GMO); 21 genetic code; 22 genome;23 helicase; 24 in vitro; 25 messenger RNA (mRNA); 26 model organisms; 27 mutations; 28 nitrogenous base; 29 nucleases; 30 nucleotide; 31neutral mutation; #s in bold are from Holt’s Modern Biology text. 1 e ...
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... Very sensitive . + rapid & time consuming test. ...
FOR IMMEDIATE RELEASE: Contact Information
FOR IMMEDIATE RELEASE: Contact Information

Chapter 20 PowerPoint
Chapter 20 PowerPoint

Chapter on Biotechnology
Chapter on Biotechnology

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Sequencing
Sequencing

... templates, we amplified overlapping fragments with an average size of 2.2 kbp (3191934070, 33005-35010, 34006-36244, 33005-34070, 34006-35438, 41312-43805, 3912941531, 35997-39437, 43518-45937) and sequenced these with internal primers. If needed, we designed species specific primers (seven for oran ...
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