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Nucleic Acid Structure:
Nucleic Acid Structure:

... replication fork to which a new nucleotide can be added. - Lagging Strand: the opposite strand, DNA synthesis must occur discontinuously (bc there is no 3’OH at the replication fork to which a new nucleotide can ...
DNA
DNA

... constituents of ribosomes, the intricate cellular machines that synthesize proteins. ...
DNA STRUCTURE AND FUNCTION
DNA STRUCTURE AND FUNCTION

... Why do we need to cool the mixture? DNases or enzymes that destroy DNA are present in the cell’s cytoplasm. They are there to protect the cell from invasion by viruses. Once the nuclear membrane is destroyed by the soap the DNA is now susceptible to the DNases and will quickly be degraded. However, ...
regulation of a bacteriophage t4 late gene, soc, which
regulation of a bacteriophage t4 late gene, soc, which

... T h e position and orientation of the soc gene are unusual for a late gene. soc maps between known early genes (see KUTTER and RUGER 1983), e.g., dam (HATTMAN1983) and mod (HORVITZ 1974). T h e restriction mapping (MACDONALD and MOSIG 1983) and sequence data (Figure 1) together with the Northern blo ...
Review sheet – Chapter 10
Review sheet – Chapter 10

... Understand that transfer RNA molecules take the information of the messenger RNA molecule and builds a polypeptide by laying down the appropriate amino acid one by one; this process is called translation and occurs at a ribosome in the cytoplasm ...
MICROBIAL GENETICS
MICROBIAL GENETICS

... The genetic informations in a cell is called the Genome. A cell's genome (chromosomes and plasmids). Chromosomes are structures containing DNA that physically carry hereditary information's; the chromosomes contain the Genes; Genes are segments of DNA The DNA within a cell exists as long strands of ...
`known unknown` genes in marine bacteria
`known unknown` genes in marine bacteria

... organisms known (2, 3). However, even within these ‘minimal’-sized genomes there exists a plethora of genes with no known biological function, so-called ‘hypothetical’ genes. This situation is magnified with the recent explosion in next generation sequencing which has led to an extensive inventory o ...
Chapter 2 Chemistry of nucleic acid
Chapter 2 Chemistry of nucleic acid

... that readily fits inside the cell  To protect DNA from damage  DNA in a chromosome can be transmitted efficiently to both daughter cells during cell division  Chromosome confers an overall organization to each molecule of DNA, which facilitates gene expression as well as recombination. ...
Tutorial: Protein Synthesis - Integrated DNA Technologies
Tutorial: Protein Synthesis - Integrated DNA Technologies

... found that many such gaps existed in genes sequenced in animals and plants. In fact, the only place where these gaps, now called introns, did not occur was in the genes of bacteria. A survey of animal and plant gene sequences also showed that all introns started with the DNA sequence GT and ended wi ...
Proteomics pathway Most common properties of proteins
Proteomics pathway Most common properties of proteins

... This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes denaturing the proteins to break non-covalent interactions, to break disulfide bonds and to disrupt non-co ...
Information Extraction from Biomedical Text
Information Extraction from Biomedical Text

... What is known about protein X (subcellular & tissue localization, associations with diseases, interactions with drugs, …)? –! assisting scientific discovery by detecting previously unknown relationships, annotating experimental data ...
The_Human_Microbiome_Congress_
The_Human_Microbiome_Congress_

... Afternoon Networking Break: Coffee, Tea & Refreshments in the Exhibition Hall Part 2: Functions of the Human Microbiome ...
DNA measurements in low volume samples
DNA measurements in low volume samples

... (A977-A900) 1cm (Water/Buffer*) ...
Some words to think about
Some words to think about

... • Remember that a nucleotide is made up of three parts: 1. Phosphate group 2. 5 carbon sugar 3. Nitrogenous base • The nitrogenous base differs • A, T, C or G in DNA • A, U, C or G in RNA ...
Biol120 Mock Final Examination (v2.0)
Biol120 Mock Final Examination (v2.0)

... 35. There are 4 alleles for a given gene within a population of snowy owls. If an individual is heterozygous for that gene, how many alleles for that gene will be present in each of their gametes? a) 1 b) 2 c) 3 d) 4 36. The term gene expression refers to the a) the flow of information through gene ...
The Blueprint of Life, From DNA to Protein
The Blueprint of Life, From DNA to Protein

... Regulation of Gene Expression • Mechanisms controlling transcription – Often controlled by regulatory region near promoter • Protein binds to region and acts as “on/off” switch – Binding protein can act as repressor or activator » Repressor blocks transcription » Activator facilitates transcription ...
Lecture 2 - CSB@Pitt
Lecture 2 - CSB@Pitt

... in lower case; non-repeating sequence is shown in upper case. • hg38.fa.masked.gz - "Hard-masked" assembly sequence in one file. Repeats are masked by capital Ns; non-repeating sequence is shown in upper case. • RepeatMasker screens DNA sequences for interspersed repeats and low complexity DNA seque ...
Microbial recognition and activation of plant defense systems
Microbial recognition and activation of plant defense systems

Full Paper - Biotechniques.org
Full Paper - Biotechniques.org

Introduction to Special Issue: A New Paradigm of Gene Therapy
Introduction to Special Issue: A New Paradigm of Gene Therapy

... The delivery systems of nucleic acids are particularly important, and in this issue we have many studies related to this issue using cationic lipids [3,4], polymers [5–7], and functional peptides [8,9]. As is well known, there is a long history of developing effective delivery systems from various f ...
Biology DNA and Protein Syn
Biology DNA and Protein Syn

... helix is separated into two individual strands. • The rules of base pairing are applied, and the result is two identical new DNA strands: each with one side from the original. ...
Lect-7
Lect-7

... host genome, a process referred to as transposition. This phage replicates by transposition.  Mu uses multiple rounds of replicate transposition to amplify it during lytic growth.  During lytic cycle Mu completes about 100 rounds of transposition per hour, making it most efficient transposition kn ...
03-131 Genes Drugs and Diseases Problem Set 7 Due November 1, 2015
03-131 Genes Drugs and Diseases Problem Set 7 Due November 1, 2015

... to tRNAPhe (tRNAPhe is the tRNA that normally brings the amino acid phenylalanine to the ribosome). The mutation causes the enzyme to also add Phe to a tRNA that has the sequence 3’-CCA-5’ as its anticodon. How will this mutation affect protein synthesis and the sequence of proteins that are produce ...
Chlamydia pneumoniae
Chlamydia pneumoniae

... • The greatest advancement in classifying organisms • Differences in the nucleotide sequence are used to classify prokaryotes Actually look at the DNA that • 16S rRNA sequences codes for the rRNA • 23S rRNA sequences ...
A Simply Fruity DNA Extraction
A Simply Fruity DNA Extraction

... All  organisms  are  made  up  of  cells,  from  simple  single-­‐cell  bacteria  to  multi-­‐cell  humans  and  plants.  It   doesn’t  matter  if  you  are  human,  a  bacteria  or  a  strawberry,  every  cell  contains  deoxyribonucleic ...
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