File - western undergrad. by the students, for the students.
... 2. The DNA sequences required for transcriptional termination are called the terminator. 3. Transcription is catalyzed by the enzyme RNA polymerase. 4. Gene specific regulatory proteins (or transcription factors) are the key molecules in the differential transcription of genes. Bacterial transcripti ...
... 2. The DNA sequences required for transcriptional termination are called the terminator. 3. Transcription is catalyzed by the enzyme RNA polymerase. 4. Gene specific regulatory proteins (or transcription factors) are the key molecules in the differential transcription of genes. Bacterial transcripti ...
Molecular Cell Biology
... RNA = ribonucleotides (OH-groups at the 2’ position) Note the directionality of DNA (5’-3’ & 3’5’) or RNA (5’-3’) DNA = A, G, C, T ; RNA = A, G, C, U ...
... RNA = ribonucleotides (OH-groups at the 2’ position) Note the directionality of DNA (5’-3’ & 3’5’) or RNA (5’-3’) DNA = A, G, C, T ; RNA = A, G, C, U ...
4.4 Genetic modification and clonng
... Steps in inserting a gene into a plasmid: 6. Ligase enzyme seals up the plasmid, returning it back to a circle. 7. Plasmids are inserted into bacteria cells. 8. Bacteria cells with plasmids & genes of interest are reproduced inside of bacteria. ...
... Steps in inserting a gene into a plasmid: 6. Ligase enzyme seals up the plasmid, returning it back to a circle. 7. Plasmids are inserted into bacteria cells. 8. Bacteria cells with plasmids & genes of interest are reproduced inside of bacteria. ...
3.5 Genetic modification and clonng 2012
... Steps in inserting a gene into a plasmid: 6. Ligase enzyme seals up the plasmid, returning it back to a circle. 7. Plasmids are inserted into bacteria cells. 8. Bacteria cells with plasmids & genes of interest are reproduced inside of bacteria. ...
... Steps in inserting a gene into a plasmid: 6. Ligase enzyme seals up the plasmid, returning it back to a circle. 7. Plasmids are inserted into bacteria cells. 8. Bacteria cells with plasmids & genes of interest are reproduced inside of bacteria. ...
An enlarged largest subunit or Plasmodium falciparum RNA
... falciparum. The RPII gene is expressed in the asexual erythrocytic stages of the parasite as a 9 kb mRNA, and is present as a single copy gene located on chromosome 3. The P. falciparum RPII subunit is the largest (2452 amino acids) eukaryotic RPII subunit, and it contains enlarged variable regions ...
... falciparum. The RPII gene is expressed in the asexual erythrocytic stages of the parasite as a 9 kb mRNA, and is present as a single copy gene located on chromosome 3. The P. falciparum RPII subunit is the largest (2452 amino acids) eukaryotic RPII subunit, and it contains enlarged variable regions ...
PASS MOCK EXAM
... • Attempt every question. After the time limit, go back over your work with a different colour or on a separate piece of paper and try to do the questions you are unsure of. Record your ideas in the margins to remind yourself of what you were thinking when you take it up at PASS. The purpose of this ...
... • Attempt every question. After the time limit, go back over your work with a different colour or on a separate piece of paper and try to do the questions you are unsure of. Record your ideas in the margins to remind yourself of what you were thinking when you take it up at PASS. The purpose of this ...
Ensembl Mart
... multiple terms and put them into a table format. • Such as: mouse gene (IDs), chromosome and base pair position • No programming required! ...
... multiple terms and put them into a table format. • Such as: mouse gene (IDs), chromosome and base pair position • No programming required! ...
protein synthesis and mutations
... The mRNA leaves the nucleus and binds to a ribosome in the cytoplasm. The ribosome reads that mRNA code in groups of 3 called codons, and tRNA hooks on the correct amino acids that is coded for. Once a stop codon is read, the polypeptide chain is released and a new protein is formed. ...
... The mRNA leaves the nucleus and binds to a ribosome in the cytoplasm. The ribosome reads that mRNA code in groups of 3 called codons, and tRNA hooks on the correct amino acids that is coded for. Once a stop codon is read, the polypeptide chain is released and a new protein is formed. ...
Network Dynamics
... becomes extremely complex. Thus a series of techniques have been evolved for analysis of metabolisms. •Kinetic Modeling Rarely undertaken since all reactions are sufficiently well known or parameters known under the different conditions (pH, temperature,..). This will change due to the rise of syste ...
... becomes extremely complex. Thus a series of techniques have been evolved for analysis of metabolisms. •Kinetic Modeling Rarely undertaken since all reactions are sufficiently well known or parameters known under the different conditions (pH, temperature,..). This will change due to the rise of syste ...
Survival of the Fittest Molecule
... of its mechanism. DNA breeding makes use of established biochemical techniques to apply strong selective pressure to molecules rather than whole animals. And because the evolution takes place in test tubes rather than in kennels, the entire process is notably faster. The basic advance of DNA breedin ...
... of its mechanism. DNA breeding makes use of established biochemical techniques to apply strong selective pressure to molecules rather than whole animals. And because the evolution takes place in test tubes rather than in kennels, the entire process is notably faster. The basic advance of DNA breedin ...
ChIP-seq - STAT 115
... • Could also be used to examine known motif enrichment • Is motif enrichment correlated with ChIP-seq enrichment? • Is motif more enriched in peak summits than peak flanks? • Motif analysis could identify transcription factor partners of ChIP-seq factors ...
... • Could also be used to examine known motif enrichment • Is motif enrichment correlated with ChIP-seq enrichment? • Is motif more enriched in peak summits than peak flanks? • Motif analysis could identify transcription factor partners of ChIP-seq factors ...
Supplementary Text S1: Detailed PCR Protocols
... first PCR products were added to each of 2 wells, mixed with 8µl PCR reaction mix [5µl of 2x Lightcycler Probe Master Mix, 0.2μM final concentration of allele specific primer set IFNB1 MF/R, and 0.1μM final concentration of Roche LNA probe #79]. The second PCR amplification was carried out using a ...
... first PCR products were added to each of 2 wells, mixed with 8µl PCR reaction mix [5µl of 2x Lightcycler Probe Master Mix, 0.2μM final concentration of allele specific primer set IFNB1 MF/R, and 0.1μM final concentration of Roche LNA probe #79]. The second PCR amplification was carried out using a ...
Cloning Vectors A cloning vector is a DNA molecule that can carry
... Cosmids are able to contain 37 to 52 kb of DNA, while normal plasmids are able to carry only 1–20 kb. They can replicate as plasmids if they have a suitable origin of replication: for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication or f1 ori for single-stranded DNA ...
... Cosmids are able to contain 37 to 52 kb of DNA, while normal plasmids are able to carry only 1–20 kb. They can replicate as plasmids if they have a suitable origin of replication: for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication or f1 ori for single-stranded DNA ...
Lecture 7: Life`s Information Molecule II
... molecule are cut out, and the other parts spliced together ...
... molecule are cut out, and the other parts spliced together ...
Supplementary Information (doc 62K)
... The second version of the micro-array contained 42 additional probes targeting micro-organisms that were selected after 454-sequencing of a highly diverse set of 100 cervicovaginal samples (from African, American and European women of different ages with and without HIV, other STIs, pelvic inflammat ...
... The second version of the micro-array contained 42 additional probes targeting micro-organisms that were selected after 454-sequencing of a highly diverse set of 100 cervicovaginal samples (from African, American and European women of different ages with and without HIV, other STIs, pelvic inflammat ...
PC Pc pC pc PC PPCC (purple) PPCc (purple) PpCC
... II-6: No, since you don’t know the parents’ genotypes (or phenotypes) so you won’t know the second allele until after they have a child. Once the child (III-4) is born homozygous recessive, then you know that II-6 must be a carrier for Tay-Sachs disease. 3) The mRNA for a newly discovered gene has a ...
... II-6: No, since you don’t know the parents’ genotypes (or phenotypes) so you won’t know the second allele until after they have a child. Once the child (III-4) is born homozygous recessive, then you know that II-6 must be a carrier for Tay-Sachs disease. 3) The mRNA for a newly discovered gene has a ...
Lect 6 JF 2012.pptx
... - Will grow if supplied with either ornithine or citrulline or arginine - Therefore the metabolic block must lie upstream of ornithine ...
... - Will grow if supplied with either ornithine or citrulline or arginine - Therefore the metabolic block must lie upstream of ornithine ...
Genetic Engineering
... History of Genetic Engineering Modern genetic engineering began in 1973 when Herbert Boyer and Stanley Cohen used enzymes to cut a bacteria plasmid and insert another strand of DNA in the gap. Both bits of DNA were from the same type of bacteria, but this milestone, the invention of recombinant DNA ...
... History of Genetic Engineering Modern genetic engineering began in 1973 when Herbert Boyer and Stanley Cohen used enzymes to cut a bacteria plasmid and insert another strand of DNA in the gap. Both bits of DNA were from the same type of bacteria, but this milestone, the invention of recombinant DNA ...
Adaptive evolution or genetic drift? Does genome
... power of random genetic drift and allowed the proliferation of genome features that would have been eliminated by purifying selection in larger populations. Once these features were in place, they provided the raw material for evolution of phenotypic complexity by natural selection. In support of th ...
... power of random genetic drift and allowed the proliferation of genome features that would have been eliminated by purifying selection in larger populations. Once these features were in place, they provided the raw material for evolution of phenotypic complexity by natural selection. In support of th ...
Part 1 – History, DNA Structure, DNA Replication
... Read the text and answer the following questions. 1. What have people wondered since the beginning of human history? _________________________________ 2. Who discovered that individual traits are passed on from one generation to the next? In what year?________________________________________________ ...
... Read the text and answer the following questions. 1. What have people wondered since the beginning of human history? _________________________________ 2. Who discovered that individual traits are passed on from one generation to the next? In what year?________________________________________________ ...
B3.3 Genetics ANSWERS Worksheet Two Molecular Genetics 1
... nucleotides. Whereas DNA replication uses DNA nucleotides to produce an identical copy. DNA replication uses both sides of the DNA, whereas transcription only uses the coding strand. The enzymes are also different; DNA replication uses helicase, DNA polymerase and DNA ligase, whereas transcription u ...
... nucleotides. Whereas DNA replication uses DNA nucleotides to produce an identical copy. DNA replication uses both sides of the DNA, whereas transcription only uses the coding strand. The enzymes are also different; DNA replication uses helicase, DNA polymerase and DNA ligase, whereas transcription u ...
concepts for episode 1 - Austin Community College
... region, making it more suitable for other kinds of organisms, and they in turn alter it by their presence and activities, making it more suitable for other kinds of organisms, etc. B. Secondary succession begins on sites where a disturbance destroys many but not all members of a community. example: ...
... region, making it more suitable for other kinds of organisms, and they in turn alter it by their presence and activities, making it more suitable for other kinds of organisms, etc. B. Secondary succession begins on sites where a disturbance destroys many but not all members of a community. example: ...
200B lect # 21 (Conservation) - Integrative Biology
... named species. There are lineages smaller and larger than the traditional species level. Furthermore, species are not comparable between lineages in any manner. Thus counting species or measuring their ranges and abundances is a poor measure of biodiversity; we must develop measures of the actual di ...
... named species. There are lineages smaller and larger than the traditional species level. Furthermore, species are not comparable between lineages in any manner. Thus counting species or measuring their ranges and abundances is a poor measure of biodiversity; we must develop measures of the actual di ...