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Supplementary Methods
Supplementary Methods

as PDF
as PDF

... essential for existence of each bacterial cell due to its involvement in protein synthesis, and their sequences are generally highly conserved among bacteria, however some variable regions are present within these sequences. The sequence of 16S rRNA gene is conserved even at the strain level. As a r ...
Question 1
Question 1

... ii) The side chain of Ser 161 interacts with the region of the substrate shown above. Draw the structure of Ser 161 and the relevant portion of the substrate as they would interact with each other. Be sure to indicate: (1) the interaction (use a dotted line) (2) the places where Ser 161 connects wit ...
2017 Lecture 10, student version
2017 Lecture 10, student version

... - born in what is now Pakistan, biochemist - worked at Univ. Wisconsin - Madison - Nobel prize in physiology or medicine 1968 with Nirenberg ...
The evolutionary history of the stearoyl
The evolutionary history of the stearoyl

... tropicalis we find a single SCD1 gene but not SCD5, though this could be due to genome incompleteness. In the analysed teleost species no SCD5 is found, while the surrounding SCD5-less locus is conserved in comparison to tetrapods. In addition, the teleost SCD1 gene repertoire expanded to two copies ...
The Carbon Cycle
The Carbon Cycle

...  Some xenobiotics can be degraded partially or completely if another organic material is present as a primary energy source (cometabolism) ...
Chapter 12 - WordPress.com
Chapter 12 - WordPress.com

... the information needed to construct a building. But builders never bring the valuable master plan to the building site, where it might be damaged or lost. Instead, they prepare inexpensive, disposable copies of the master plan called blueprints. The master plan is safely stored in an office, and the ...
PORTER, KAREN G., AND YVETTE S. FEIG. The use of DAPI for
PORTER, KAREN G., AND YVETTE S. FEIG. The use of DAPI for

... time required for staining, we stained samples for 2,5, and 30 min, and 2 and 24 h with DAPI. Stain was applied in the filter apparatus for 2 and 5 min and to samples stored in vials at 4°C for 0.5, 2, and 24 h. The stored samples were allowed to stand in the filter apparatus for 5 min before filtra ...
Official pGLO GFP powerpoint Spring 2005
Official pGLO GFP powerpoint Spring 2005

... Central Framework of Molecular Biology ...
Hydrogen Sulfide Sewer IND 08 Case Study
Hydrogen Sulfide Sewer IND 08 Case Study

... control of grease, fats, oils and hydrogen sulfide generation within city sewers, wet wells, lift stations and wastewater systems. MICROBE-LIFT/IND is designed specific to control the odor related to the production of hydrogen sulfide within anaerobic systems and processes. MICROBE-LIFT/IND differs ...
PowerPoint 簡報
PowerPoint 簡報

... Patterns—>evolutionary processes? ...
DNA
DNA

... 2. Translation uses the mRNA to build a protein a. In the cytoplasm of the cell, translation occurs at the ribosome. Ribosomes are made of rRNA (ribosomal RNA) and proteins. b. The mRNA “start” codon attaches to the ribosome. The ribosome holds mRNA in place and helps link amino acids together to ma ...
File
File

... 26. You have just hooked 4 amino acids together in the process of protein synthesis. This was done using the “DNA code” found on the genes. Hopefully, you have discovered how important the exact sequence of nucleotides is to this process. For example: The DNA code on the portion of the globin gene w ...
LIGATION AND TRANSFORMATION
LIGATION AND TRANSFORMATION

... Two crucial procedures in cloning are the ligation of the foreign DNAs to the vector DNA and the transformation of bacteria using those ligated DNA constructs (the recombinant molecules). Ligation is accomplished using the enzyme DNA ligase (usually from the bacteriophage T4). It requires ATP and ma ...
Scanning promoters to predict TF binding sites and
Scanning promoters to predict TF binding sites and

... Choose the option 'CDS' (coding sequence). In the menu sequence type, select the option 'upstream'. For the next option 'From' and 'to', you can click on them to see the suggested positions for TF activators or repressors. To know if your selected TF is activator or repressor you can search in Regul ...
Bacterial Growth Metabolism  - King George`s Medical University
Bacterial Growth Metabolism - King George`s Medical University

... • Families - related bacteria eg. Enterobacteriaceae • Within a family - numerous genus eg. Escherichia, Klebsiella, Salmonella ...
Biology Dictionary
Biology Dictionary

... phosphate and the pentose sugar deoxyribose. DNA is the genetic material of most organisms and usually exists as a double-stranded molecule in which two antiparallel strands are held together by hydrogen bonds between adeninethymine and cytosine-guanine. (See b-DNA, cDNA, Complementary DNA or RNA, D ...
Project: Large scale identification of MHC haplotypes in diverse
Project: Large scale identification of MHC haplotypes in diverse

... The objective of this project is to generate full length MHC gene sequences from a range of diverse cattle breeds using next generation sequencing (NGS) methods. A recently published study established a highthroughput protocol to determine MHC class I allele fragments from cDNA (Vasoya et al. 2016). ...
Document
Document

... Construction of Genomic DNA and cDNA libraries, screening of recombinants. DNA analysis: labeling of DNA and RNA probes. Southern and fluorescence in situ hybridization, DNA fingerprinting, chromosome walking. Techniques for gene expression: Northern and Western blotting, gel retardation technique, ...
Document
Document

... Please follow the "BEH.109 Guidelines for Module Reports" handout that was given to you previously. The report will be DUE ON MONDAY, APRIL 29th BY 5 PM. ...
Gene mutation and DNA polymorphism
Gene mutation and DNA polymorphism

... The occurrence in a population of two or more genetically determined forms in such frequencies that the rarest of them could not be maintained by mutation alone A polymorphic locus is one at which there are at least two alleles, each with a frequency greater than 1%. Alleles with frequencies less th ...
Cloning and characterization of CmGPD1, the Candida magnoliae
Cloning and characterization of CmGPD1, the Candida magnoliae

... No claim to original Korean government works ...
control of gene expression
control of gene expression

Molecular identification of tephritid fruit flies
Molecular identification of tephritid fruit flies

... The exotic tephritid Bactrocera invadens (syn. Bactrocera dorsalis) and the native Ceratitis species constitute serious pests and major impediment to Citrus production in Nigeria. Lack of accurate and reliable diagnostic methods independent of the life stages intercepted for both genera hampers impl ...
Worksheet 2
Worksheet 2

... The lowest position no. of these four numbers is: __________________ The highest position no. of these four numbers is: __________________ These two numbers stretch a region of how many nucleotides? __________________ Record the gi-number for this entry: _______________________________________ What ...
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