Construction of recombinant plasmid carrying the λ DNA fragment
... cells is one of the major problems of recombinant DNA research. The stabilization of foreign DNA sequences in the cell can be achieved by integration of these sequences into host chromosome. It may be carried out by means of A vectors containing the genes necessary for stable lysogenization. However ...
... cells is one of the major problems of recombinant DNA research. The stabilization of foreign DNA sequences in the cell can be achieved by integration of these sequences into host chromosome. It may be carried out by means of A vectors containing the genes necessary for stable lysogenization. However ...
Self-Adaptation of Genome Size in Artificial Organisms
... stabilise genome size. Other hypotheses accounting for the steady genome sizes of natural organisms involve natural selection acting directly at the genomic level, either as a stabilising force maintaining the DNA content at a physiological optimum [9], or as a directional pressure counterbalancing ...
... stabilise genome size. Other hypotheses accounting for the steady genome sizes of natural organisms involve natural selection acting directly at the genomic level, either as a stabilising force maintaining the DNA content at a physiological optimum [9], or as a directional pressure counterbalancing ...
Biofuel alternatives to ethanol: pumping the microbial well
... that have higher growth-rates, better yields, lower soil-impacts, and lower water, fertilizer and pesticide requirements than currently used crops such as oil palms, corn or soybeans [67]. These characteristics should serve to make biofuels more competitive with petroleum, while making them less com ...
... that have higher growth-rates, better yields, lower soil-impacts, and lower water, fertilizer and pesticide requirements than currently used crops such as oil palms, corn or soybeans [67]. These characteristics should serve to make biofuels more competitive with petroleum, while making them less com ...
Molecular Plant-Microbe Interaction
... tropicii, and Rhizobium etli, which remained in the Rhizobium genus. Azorhizobium caulinodans falls into a separate genus and the phylogenetic position of Rhizobium galageae is still unclear, being as distant from Agrobacterium as it is from Rhizobium. In this paper, representative species from each ...
... tropicii, and Rhizobium etli, which remained in the Rhizobium genus. Azorhizobium caulinodans falls into a separate genus and the phylogenetic position of Rhizobium galageae is still unclear, being as distant from Agrobacterium as it is from Rhizobium. In this paper, representative species from each ...
26493 Purify Nucleic Acids
... of study leading to that assessment. Industry Training Organisations must be granted consent to assess against standards by NZQA before they can register credits from assessment against unit standards. Providers and Industry Training Organisations, which have been granted consent and which are asses ...
... of study leading to that assessment. Industry Training Organisations must be granted consent to assess against standards by NZQA before they can register credits from assessment against unit standards. Providers and Industry Training Organisations, which have been granted consent and which are asses ...
PDF Links - Journal of the Korean Ceramic Society
... The addition of Asn, Gly and Asp into HAp/GEL nanocomposites could be analyzed by using XRD and FT-IR. One effect of side-group additives is on the organic-inorganic interaction between HAp and the GEL matrix. Another effect is on the organic-organic interaction between the GEL macromolecules matrix ...
... The addition of Asn, Gly and Asp into HAp/GEL nanocomposites could be analyzed by using XRD and FT-IR. One effect of side-group additives is on the organic-inorganic interaction between HAp and the GEL matrix. Another effect is on the organic-organic interaction between the GEL macromolecules matrix ...
Patterns of cooccurrences in a killifish
... To analyse the effect of organism size on community structure, we sorted all individuals by biovolume and then grouped them into a number of size-classes that exactly matched the number of species observed in each sample. For each sampling occasion, we constructed four presence (1)/ absence (0) matr ...
... To analyse the effect of organism size on community structure, we sorted all individuals by biovolume and then grouped them into a number of size-classes that exactly matched the number of species observed in each sample. For each sampling occasion, we constructed four presence (1)/ absence (0) matr ...
DNA Replication and Telomere Maintenance
... • Internal sites on linear chromosomes. • Mice have 25,000 origins, spanning ~150 kb each. • Humans have 10,000 to 100,000 origins. ...
... • Internal sites on linear chromosomes. • Mice have 25,000 origins, spanning ~150 kb each. • Humans have 10,000 to 100,000 origins. ...
Intrastrand Self-complementary Sequences in Bacillus subtilis DNA
... 60 % of the HA HI1 DNA was resistant to S 1 nuclease, whereas thermal denaturation profiles (data not shown) indicated that only 25 % of HA HI1 DNA forms helical regions, suggesting that the resistant DNA contains single-stranded, presumably unpaired, loop regions. Thus the self-complementary sequen ...
... 60 % of the HA HI1 DNA was resistant to S 1 nuclease, whereas thermal denaturation profiles (data not shown) indicated that only 25 % of HA HI1 DNA forms helical regions, suggesting that the resistant DNA contains single-stranded, presumably unpaired, loop regions. Thus the self-complementary sequen ...
Lecture 8
... gradiennt in the presence of high voltage. • Can also use gels with immobilized pH gradients -made of acrylamide derivatives that are covalently linked to ampholytes. Used with a gradient maker to ensure continuously varied mixture when the gel is made. ...
... gradiennt in the presence of high voltage. • Can also use gels with immobilized pH gradients -made of acrylamide derivatives that are covalently linked to ampholytes. Used with a gradient maker to ensure continuously varied mixture when the gel is made. ...
MGI-Jan2007 - Gene Ontology Consortium
... as well as the provision of definitions for many existing cell types, is_a links to root for all hemopoietic cell types, and extensive develops_from links for many cell lineages. This revision was implemented in June 2006. The immunologically related GO process terms involving cell types are now in ...
... as well as the provision of definitions for many existing cell types, is_a links to root for all hemopoietic cell types, and extensive develops_from links for many cell lineages. This revision was implemented in June 2006. The immunologically related GO process terms involving cell types are now in ...
Comparing the Prevalence of Verotoxin
... Multiplex PCR uses probes and molecular beacons to select specific DNA regions from specific pathogens and amplify them simultaneously. As amplification increases, so does the fluorescence of the probes, allowing for the quantitative measurement of multiple pathogens. ...
... Multiplex PCR uses probes and molecular beacons to select specific DNA regions from specific pathogens and amplify them simultaneously. As amplification increases, so does the fluorescence of the probes, allowing for the quantitative measurement of multiple pathogens. ...
Chapter 18: REGULATION OF GENE EXPRESSION
... as a single unit (encoding proteins of the same metabolic pathway). A regulator gene, located outside the operon, codes for a repressor that controls whether the operon is active or not. An operon includes the following elements: Promoter—A short sequence of DNA where RNA polymerase first attach ...
... as a single unit (encoding proteins of the same metabolic pathway). A regulator gene, located outside the operon, codes for a repressor that controls whether the operon is active or not. An operon includes the following elements: Promoter—A short sequence of DNA where RNA polymerase first attach ...
Monoamine Oxidase B Enzyme Specific Activity
... Note: This protocol contains detailed steps for measuring MAOB activity in human samples. Be completely familiar with the protocol before beginning the assay. Do not deviate from the specified protocol steps or optimal results may not be obtained. A. Preparation of buffers 1. Prepare 1X Wash Buffer ...
... Note: This protocol contains detailed steps for measuring MAOB activity in human samples. Be completely familiar with the protocol before beginning the assay. Do not deviate from the specified protocol steps or optimal results may not be obtained. A. Preparation of buffers 1. Prepare 1X Wash Buffer ...
Transformation of Clostridium Thermocellum by Electroporation
... 1000 bp in length and are referred to as “50 flank” and “30 flank.” Additionally, there is a third region with homology to the gene target. Typically, this region referred to as the “int region” is internal to the gene of interest and is also 500–1000 bp in length. Note: if the gene target is <500 b ...
... 1000 bp in length and are referred to as “50 flank” and “30 flank.” Additionally, there is a third region with homology to the gene target. Typically, this region referred to as the “int region” is internal to the gene of interest and is also 500–1000 bp in length. Note: if the gene target is <500 b ...
Inquiry into Life Twelfth Edition
... – E site is inverted relative to its position in the A site (active) where phosphodiester bonds form – E and A sites partially overlap – Rotation of nucleotide between the E and A sites may play a role in base and sugar specificity ...
... – E site is inverted relative to its position in the A site (active) where phosphodiester bonds form – E and A sites partially overlap – Rotation of nucleotide between the E and A sites may play a role in base and sugar specificity ...
Microbial Degradation of Cycloparaffinic
... 1973), cyclohexane may lack the capacity to induce the requisite oxidative enzymes responssible for its degradation. Studies are being continued on this aspect of cycloparaffin metabolism. The inability to isolate organisms from soil that can utilize cycloparaffinic hydrocarbons as carbon and energy ...
... 1973), cyclohexane may lack the capacity to induce the requisite oxidative enzymes responssible for its degradation. Studies are being continued on this aspect of cycloparaffin metabolism. The inability to isolate organisms from soil that can utilize cycloparaffinic hydrocarbons as carbon and energy ...
Microbial Degradation of Cycloparaffinic
... 1973), cyclohexane may lack the capacity to induce the requisite oxidative enzymes responssible for its degradation. Studies are being continued on this aspect of cycloparaffin metabolism. The inability to isolate organisms from soil that can utilize cycloparaffinic hydrocarbons as carbon and energy ...
... 1973), cyclohexane may lack the capacity to induce the requisite oxidative enzymes responssible for its degradation. Studies are being continued on this aspect of cycloparaffin metabolism. The inability to isolate organisms from soil that can utilize cycloparaffinic hydrocarbons as carbon and energy ...
Abstract - Site de utilizadores
... fluorometric methods for the extraction and quantification of nucleic acids in individual estuarine gobiid larvae are compared. A total of 141 gobiid larvae, collected in the Mira and Guadiana estuaries (Portugal), were analysed. This study indicated that the nucleic acids content and RNA/DNA ratios ...
... fluorometric methods for the extraction and quantification of nucleic acids in individual estuarine gobiid larvae are compared. A total of 141 gobiid larvae, collected in the Mira and Guadiana estuaries (Portugal), were analysed. This study indicated that the nucleic acids content and RNA/DNA ratios ...