Association of polymorphisms in kappa casein gene with milk traits
... substitution effect) were estimated through adding an additional regression covariate with value 0, 1 and 2 to account for number of different genotypes. ...
... substitution effect) were estimated through adding an additional regression covariate with value 0, 1 and 2 to account for number of different genotypes. ...
Identification of the five most common cystic fibrosis mutations in
... of the most common mutations in the standard kit, and up to 12 mutations in the standard plus kit (Cellmark), but neither has been adapted to detect these mutations from a single cell as a diagnostic procedure. The ARMS procedure relies on the introduction of a mismatch between a polmerase chain rea ...
... of the most common mutations in the standard kit, and up to 12 mutations in the standard plus kit (Cellmark), but neither has been adapted to detect these mutations from a single cell as a diagnostic procedure. The ARMS procedure relies on the introduction of a mismatch between a polmerase chain rea ...
CF Overview of CF Genotypin and NSQAP services
... Was the baby homozygous or hemizygous for Y1092H T>C? hemizygous is when there is only 1 member of a chromosome segment rather than the usual 2 ...
... Was the baby homozygous or hemizygous for Y1092H T>C? hemizygous is when there is only 1 member of a chromosome segment rather than the usual 2 ...
Supplementary Methods Tables
... The open access tier is publically accessible, and contains data that are considered by TCGA to present a low risk of re-identification of individual participants. The open access data tier does not require user certification for data access. The controlled access tier contains data, including all ...
... The open access tier is publically accessible, and contains data that are considered by TCGA to present a low risk of re-identification of individual participants. The open access data tier does not require user certification for data access. The controlled access tier contains data, including all ...
The_RAY_Manual
... ii) mutations engineered into the recombinogenic arms will get incorporated into the gene segment with a frequency inversly related to their distance from the genetic elements. Selection strategies The recombination frequencies we observed are high enough to allow a PCR-based identification of posit ...
... ii) mutations engineered into the recombinogenic arms will get incorporated into the gene segment with a frequency inversly related to their distance from the genetic elements. Selection strategies The recombination frequencies we observed are high enough to allow a PCR-based identification of posit ...
Chapter 20~ DNA Technology & Genomics
... DNA ligase joins the strands sticky ends stick together ...
... DNA ligase joins the strands sticky ends stick together ...
DNA Shape Dominates Sequence Affinity in Nucleosome Formation
... evidence produced over the last few years suggests that nucleosome preference is directly encoded by DNA [6–9]. Different sequence motifs possess unique structural properties—intrinsic curvature, minor groove dimensions, and local flexibility—that render them more or less favorable for protein bindi ...
... evidence produced over the last few years suggests that nucleosome preference is directly encoded by DNA [6–9]. Different sequence motifs possess unique structural properties—intrinsic curvature, minor groove dimensions, and local flexibility—that render them more or less favorable for protein bindi ...
University of Bucharest, Faculty of Biology, Molecular Biology Center
... 136bp. After endonuclease digestion two fragments of 108 and 28bp characteristics for the normal cattle were detected (figure 2). Normal homozygote for DUMPS should shown three bands of 51, 36 and 21bp, carrier heterozygote four bands of 87, 51, 36 and 21bp and affected homozygote only two bands of ...
... 136bp. After endonuclease digestion two fragments of 108 and 28bp characteristics for the normal cattle were detected (figure 2). Normal homozygote for DUMPS should shown three bands of 51, 36 and 21bp, carrier heterozygote four bands of 87, 51, 36 and 21bp and affected homozygote only two bands of ...
Identification of markers tightly linked to tomato yellow
... The tomato (Solanum lycopersicon) is an economically important species of the Solanaceae family, and it is cultivated all over the world for human consumption. Recently, tomato crops have often been infected by tomato yellow leaf curl virus (TYLCV), which causes significant yield losses in tomato (S ...
... The tomato (Solanum lycopersicon) is an economically important species of the Solanaceae family, and it is cultivated all over the world for human consumption. Recently, tomato crops have often been infected by tomato yellow leaf curl virus (TYLCV), which causes significant yield losses in tomato (S ...
Chromosomes Identification
... FISH applies molecular genetic techniques to chromosome preparations in metaphase or interphase nuclei, an approach called molecularcytogenetics. The aim is to to map genes and to detect small chromosomal rearrangements that cannot be detected by microscopy . Conventional chromosomal analysis can de ...
... FISH applies molecular genetic techniques to chromosome preparations in metaphase or interphase nuclei, an approach called molecularcytogenetics. The aim is to to map genes and to detect small chromosomal rearrangements that cannot be detected by microscopy . Conventional chromosomal analysis can de ...
A simplified subtractive hybridization protocol used to isolate DNA
... A simplified protocol of subtractive hybridization based on the technique of L. M. Kunkel, A. P. Monaco, W. Middlesworth, H. D. Ochs & 5. A. Latt (1985, Proc Natl Acad Sci USA, 82,47784782) was used to obtain DNA sequences specific to Xy/el/a fastidiosa isolated from diseased citrus plants. As a dri ...
... A simplified protocol of subtractive hybridization based on the technique of L. M. Kunkel, A. P. Monaco, W. Middlesworth, H. D. Ochs & 5. A. Latt (1985, Proc Natl Acad Sci USA, 82,47784782) was used to obtain DNA sequences specific to Xy/el/a fastidiosa isolated from diseased citrus plants. As a dri ...
Cloning of genes from genomic DNA Parts 4 and 5: Ligation and
... Ligations. In addition, Tina and I (which really means Tina) have linearized the plasmid vector, pBluescript, by cutting it with the appropriate enzymes (XbaI and HinDIII). This week, we will ligate the cut PCR product and the cut vector together, thereby cloning our gene. The “sticky” ends (5’ over ...
... Ligations. In addition, Tina and I (which really means Tina) have linearized the plasmid vector, pBluescript, by cutting it with the appropriate enzymes (XbaI and HinDIII). This week, we will ligate the cut PCR product and the cut vector together, thereby cloning our gene. The “sticky” ends (5’ over ...
Sheared DNA fragment sizing: comparison of techniques
... Fig. 4 Size Distribution of Sheared DNA Fragments. Panels a, b and c show the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative m ...
... Fig. 4 Size Distribution of Sheared DNA Fragments. Panels a, b and c show the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative m ...
An exo-b-( 1,3)-glucanase of Candida albicans
... Determination of enzyme activity. Exoglucanase activity was determined with laminaran, 2 mg ml-', in 80 mM-sodium acetate buffer, pH 5.6. Assays (250 pl) were incubated for 30 min at 37 "C and the reactions were stopped by heating to 100 "C for 10 min. Glucose formation was measured by the glucose o ...
... Determination of enzyme activity. Exoglucanase activity was determined with laminaran, 2 mg ml-', in 80 mM-sodium acetate buffer, pH 5.6. Assays (250 pl) were incubated for 30 min at 37 "C and the reactions were stopped by heating to 100 "C for 10 min. Glucose formation was measured by the glucose o ...
Exome Sequencing Project release
... at American Society of Human Genetics 2012 meeting A multi-institutional team of researchers has sequenced the DNA of 6,700 exomes, the portion of the genome that contains protein-coding genes, as part of the National Heart, Lung and Blood Institute (NHLBI)-funded Exome Sequencing Project, one of th ...
... at American Society of Human Genetics 2012 meeting A multi-institutional team of researchers has sequenced the DNA of 6,700 exomes, the portion of the genome that contains protein-coding genes, as part of the National Heart, Lung and Blood Institute (NHLBI)-funded Exome Sequencing Project, one of th ...
Mutations in a gene encoding a novel protein tyrosine
... identity with transcript A, except for the omission of a 1,770-bp segment due to splicing (Figs 3, 4). The common origin of transcripts A and B suggests they are alternative forms of the same gene, the products of which would be predicted to have unique carboxy-terminal amino acid sequences (Fig. 4b ...
... identity with transcript A, except for the omission of a 1,770-bp segment due to splicing (Figs 3, 4). The common origin of transcripts A and B suggests they are alternative forms of the same gene, the products of which would be predicted to have unique carboxy-terminal amino acid sequences (Fig. 4b ...
Direct Sequence Analysis of the 14q+ and 18q
... volume included 1 pg of tumor DNA, oligonucleotide primers (1 60% of the breakpoints occur within 150 base pairs (bp) mmol/L) (Boehringer Mannheim, FRG), 1.5 U of Taq DNA known as the “major breakpoint region” (mbr) in a 3’ polymerase (Amplitaq-Cetus),and gelatin (0.01% wt/vol) in 100 r L untranslat ...
... volume included 1 pg of tumor DNA, oligonucleotide primers (1 60% of the breakpoints occur within 150 base pairs (bp) mmol/L) (Boehringer Mannheim, FRG), 1.5 U of Taq DNA known as the “major breakpoint region” (mbr) in a 3’ polymerase (Amplitaq-Cetus),and gelatin (0.01% wt/vol) in 100 r L untranslat ...
Analysis of the DNA microarray hybridization images using
... many thousands of spots. Main idea of microarray experiment relies on hybridization of known DNA strands from BioChip with an unknown and fluorescently tagged nucleic acid sample in solution. After hybridization, the microarray is scanned using specialized scanners. The obtained hybridization pictur ...
... many thousands of spots. Main idea of microarray experiment relies on hybridization of known DNA strands from BioChip with an unknown and fluorescently tagged nucleic acid sample in solution. After hybridization, the microarray is scanned using specialized scanners. The obtained hybridization pictur ...
Lab 6: Electrophoresis
... helix at the same position on both strands to produce fragments with blunt ends (Figure 1). Other endonucleses cleave each strand off-center at specific nucleotides to produce fragments with “overhangs” or sticky ends. By using the same restriction enzyme to “cut” DNA from two different organisms, c ...
... helix at the same position on both strands to produce fragments with blunt ends (Figure 1). Other endonucleses cleave each strand off-center at specific nucleotides to produce fragments with “overhangs” or sticky ends. By using the same restriction enzyme to “cut” DNA from two different organisms, c ...
Lab Recap: Miniprep (MP)
... In PCR, you added a bunch of stuff to a tube and a machine made all the PCR reactions happen. We gave you a bunch of notes explaining how the PCR process was going to happen on a molecular level, but you never saw any of the important reactions taking place. The one problem with the PCR lab is th ...
... In PCR, you added a bunch of stuff to a tube and a machine made all the PCR reactions happen. We gave you a bunch of notes explaining how the PCR process was going to happen on a molecular level, but you never saw any of the important reactions taking place. The one problem with the PCR lab is th ...
file - UCL Discovery
... To perform synteny analysis, you will need to repeat the above analysis for equivalent regions in other species of interest. Once you have this done, you will have CTCF predicted results from all species. The next step is to look at synteny across these species to see whether the same set of genes i ...
... To perform synteny analysis, you will need to repeat the above analysis for equivalent regions in other species of interest. Once you have this done, you will have CTCF predicted results from all species. The next step is to look at synteny across these species to see whether the same set of genes i ...
Open Access - Scientific Research Publishing
... A.A. (2008) Database Indexing for Production MegaBLAST Searches. Bioinformatics, 24, 1757-1764. [13] Choi, Y., Sims, G.E., Murphy, S., Miller, J.R. and Chan, A.P. (2012) Predicting the Functional Effect of Amino Acid Substitutions and Indels. PLoS ONE, 7, e46688. ...
... A.A. (2008) Database Indexing for Production MegaBLAST Searches. Bioinformatics, 24, 1757-1764. [13] Choi, Y., Sims, G.E., Murphy, S., Miller, J.R. and Chan, A.P. (2012) Predicting the Functional Effect of Amino Acid Substitutions and Indels. PLoS ONE, 7, e46688. ...
NOTE slides 15-21
... What information did they use and where did they get it? Describe this model, in general. What two major functions of DNA did this model support? What did they win? Who didn't win that should have? ...
... What information did they use and where did they get it? Describe this model, in general. What two major functions of DNA did this model support? What did they win? Who didn't win that should have? ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).