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DNA Test Study Guide
DNA Test Study Guide

... 22. What is the importance of mRNA to the protein making process? 23. Use the codon chart on the next page to look up amino acids coded for by this mRNA strand: UUA AAA GCG __________ ____________ _____________ 24. What is the final product of protein synthesis?_______________ 25. If you took a pic ...
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... before addition of secondary antibodies bound to DNA:Au nanoparticle conjugates. • Unreacted Au nanoparticle conjugates are removed after magnetic separation, then elevated temperature release the barcode DNA for analysis. • Each Au nanosphere carries hundreds of identical barcode DNA strands, provi ...
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Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
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