Lecture 10: Nucleic acids (DNA & RNA)
... deoxyribose, which contains a hydrogen (H) at the second (2') carbon. The sugar in RNA is ribose, which contains a hydroxyl (OH) group at the 2' carbon. The carbons of the sugars are numbered 1 to 5. ...
... deoxyribose, which contains a hydrogen (H) at the second (2') carbon. The sugar in RNA is ribose, which contains a hydroxyl (OH) group at the 2' carbon. The carbons of the sugars are numbered 1 to 5. ...
lesson viii - MisterSyracuse.com
... 8. So there is a helicase that opens up the DNA, and then other proteins like gyrases and single-strand binding proteins to keep it open. 9. This time, though, we are making only a single-stranded copy. So we only need to use one side, and there will be only one leading strand. No Okazaki fragments ...
... 8. So there is a helicase that opens up the DNA, and then other proteins like gyrases and single-strand binding proteins to keep it open. 9. This time, though, we are making only a single-stranded copy. So we only need to use one side, and there will be only one leading strand. No Okazaki fragments ...
File
... 2. This causes the DNA to unzip like a zipper. 3. Enzymes in the nucleus called _DNA polymerase directs free floating nucleotides in the nucleus to attach to each strand following the rules of base pairing. 4. Each strand serves as a template for the new strand. 5. This results in two identical stra ...
... 2. This causes the DNA to unzip like a zipper. 3. Enzymes in the nucleus called _DNA polymerase directs free floating nucleotides in the nucleus to attach to each strand following the rules of base pairing. 4. Each strand serves as a template for the new strand. 5. This results in two identical stra ...
QCM2 - GIGA
... genetically linked with the disease gene in order to determine its chromosomal location, a procedure known as mapping. ...
... genetically linked with the disease gene in order to determine its chromosomal location, a procedure known as mapping. ...
Chapter 8 Bacterial Genetics
... You should be able to find the information necessary to answer these questions in Tortora, Funke, and Case, or in lecture. However, for a fuller understanding of the concept, or to add more detail to your answer you are encouraged to use other sources (see on-line resources by chapter) 1. Use exampl ...
... You should be able to find the information necessary to answer these questions in Tortora, Funke, and Case, or in lecture. However, for a fuller understanding of the concept, or to add more detail to your answer you are encouraged to use other sources (see on-line resources by chapter) 1. Use exampl ...
Introduction to high-‐throughput experiments and data analysis
... DNA microarrays • A collection many spots, each has a certain type of probes (short segments of DNAs). • Detect and quantify target sequence (e.g., mRNA) by hybridization: sequence-‐specific interaction between two ...
... DNA microarrays • A collection many spots, each has a certain type of probes (short segments of DNAs). • Detect and quantify target sequence (e.g., mRNA) by hybridization: sequence-‐specific interaction between two ...
Restriction Digestion and Analysis of Lambda DNA
... nucleotides. Each reaction contains the DNA polymerase, the single-stranded DNA template to be sequenced to which a synthetic primer has been hybridized, the four deoxyribonucleotide triphosphates (dATP, dGTP, dCTP, dTTP), often ad isotopically labeled deoxynucleotide triphosphate, such as 32P, and ...
... nucleotides. Each reaction contains the DNA polymerase, the single-stranded DNA template to be sequenced to which a synthetic primer has been hybridized, the four deoxyribonucleotide triphosphates (dATP, dGTP, dCTP, dTTP), often ad isotopically labeled deoxynucleotide triphosphate, such as 32P, and ...
Intermediate Inheritance or Incomplete Dominance
... • Injected a mix of heat-killed S bacteria and living R bacteria into mice • Hypothesized mice would not be affected by the mixture • However – mice died of pneumonia • What killed the mice? • Allowed bacteria from dead mice to reproduce • Offspring had the mucous coats • Reasoned somehow a transfor ...
... • Injected a mix of heat-killed S bacteria and living R bacteria into mice • Hypothesized mice would not be affected by the mixture • However – mice died of pneumonia • What killed the mice? • Allowed bacteria from dead mice to reproduce • Offspring had the mucous coats • Reasoned somehow a transfor ...
Foundations in Microbiology
... removal of genetic material from one organism and combining it with that of a different organism – Objective of recombinant technology is cloning which requires that the desired donor gene be selected, excised by restriction endonucleases, and isolated. – The gene is inserted into a vector (plasmid, ...
... removal of genetic material from one organism and combining it with that of a different organism – Objective of recombinant technology is cloning which requires that the desired donor gene be selected, excised by restriction endonucleases, and isolated. – The gene is inserted into a vector (plasmid, ...
As a group, quietly discuss each question and agree
... • As a group, quietly discuss each question and agree upon one correct answer. The group with the most correct answers will win. ...
... • As a group, quietly discuss each question and agree upon one correct answer. The group with the most correct answers will win. ...
4A DNA Pre-Standard ANSWER KEY DNA STRUCTURE What type
... 10. What molecules make up the rungs of a DNA molecule? NITROGEN BASES BONDED TOGETHER WITH HYDROGEN BONDS 11. What are the complementary base pairs? (Which base pairs with which?) A-T. G-C ...
... 10. What molecules make up the rungs of a DNA molecule? NITROGEN BASES BONDED TOGETHER WITH HYDROGEN BONDS 11. What are the complementary base pairs? (Which base pairs with which?) A-T. G-C ...
13. DNA Replication
... 1. Review of DNA structure DNA double helix model: DNA made of nucleotide building blocks linked into polymer chains Bases are on inside, sugars and phosphates form a backbone on outside Two strands exist in an antiparallel arrangement ...
... 1. Review of DNA structure DNA double helix model: DNA made of nucleotide building blocks linked into polymer chains Bases are on inside, sugars and phosphates form a backbone on outside Two strands exist in an antiparallel arrangement ...
Answer all the questions Time allowed : 49 minutes 1. State two
... Each DNA molecule is formed from two complementary polynucleotide chains running anti-parallel to each other. In a polynucleotide chain, adjacent nucleotides are joined together by phosphodiester bridges . A phosphate gro up, a deoxyribose and a nitrogenous base join together to form a nucleotide. P ...
... Each DNA molecule is formed from two complementary polynucleotide chains running anti-parallel to each other. In a polynucleotide chain, adjacent nucleotides are joined together by phosphodiester bridges . A phosphate gro up, a deoxyribose and a nitrogenous base join together to form a nucleotide. P ...
tested
... 2. SEQUENCE ANALYSES - Human and chimp DNA is 98.4% similar in nitrogenous base sequence. - well, we are similar (mammals, primates, etc.) So, to be similar, don’t we need similar recipes? ...
... 2. SEQUENCE ANALYSES - Human and chimp DNA is 98.4% similar in nitrogenous base sequence. - well, we are similar (mammals, primates, etc.) So, to be similar, don’t we need similar recipes? ...
notes_14C_nucacids
... nucleic acids supercoil and wrap around histones (proteins) - In eukaryotic cells (plants, animals, fungi, & protists), DNA is located in the cell nucleus. - In prokaryotic cells (eubacteria & archaea), DNA is located in the nucleoid; there is no nuclear envelope to separate DNA from the cytoplasm. ...
... nucleic acids supercoil and wrap around histones (proteins) - In eukaryotic cells (plants, animals, fungi, & protists), DNA is located in the cell nucleus. - In prokaryotic cells (eubacteria & archaea), DNA is located in the nucleoid; there is no nuclear envelope to separate DNA from the cytoplasm. ...
Nucleotides and Nucleic Acids
... nucleic acids supercoil and wrap around histones (proteins) - In eukaryotic cells (plants, animals, fungi, & protists), DNA is located in the cell nucleus. - In prokaryotic cells (eubacteria & archaea), DNA is located in the nucleoid; there is no nuclear envelope to separate DNA from the cytoplasm. ...
... nucleic acids supercoil and wrap around histones (proteins) - In eukaryotic cells (plants, animals, fungi, & protists), DNA is located in the cell nucleus. - In prokaryotic cells (eubacteria & archaea), DNA is located in the nucleoid; there is no nuclear envelope to separate DNA from the cytoplasm. ...
Electrochemical DNA Biosensors
... • Adsorptive accumulation onto carbon-paste or disposable strip electrodes. ...
... • Adsorptive accumulation onto carbon-paste or disposable strip electrodes. ...
DNA
... 3. What is the function of RNA? _____________________________ A random change in the DNA code 4. What is a mutation? ______________________________________ 5. What term best describes what a DNA looks like? _________________ Double helix 6. Explain the relationship between DNA, chromosomes, and gene ...
... 3. What is the function of RNA? _____________________________ A random change in the DNA code 4. What is a mutation? ______________________________________ 5. What term best describes what a DNA looks like? _________________ Double helix 6. Explain the relationship between DNA, chromosomes, and gene ...
DNA Extraction Lab
... Observations: What do the following items look like? Stage of Procedure Mashed Strawberry Filtered Strawberry Strawberry with Extraction Solution Strawberry with Isopropyl Alcohol DNA ...
... Observations: What do the following items look like? Stage of Procedure Mashed Strawberry Filtered Strawberry Strawberry with Extraction Solution Strawberry with Isopropyl Alcohol DNA ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).