Viability and Cytotoxicity Assay Kits for Diverse Cell Types
... Reduced handling risks. This kit allows viability staining to take place while the potentially pathogenic sample is well contained. Subsequent treatment with 4% glutaraldehyde (or less effectively with formaldehyde) permits safer handling during analysis, without disrupting the distinctive staining ...
... Reduced handling risks. This kit allows viability staining to take place while the potentially pathogenic sample is well contained. Subsequent treatment with 4% glutaraldehyde (or less effectively with formaldehyde) permits safer handling during analysis, without disrupting the distinctive staining ...
Competition with pathogens in mucosal colonization
... understanding of the microbial ecology of the gastrointestinal tract. The normal resident gastrointestinal microflora contains many diverse populations of bacteria which play an essential role in the development and well being of the host [67]. By continual release of antibiotic proteins, specialize ...
... understanding of the microbial ecology of the gastrointestinal tract. The normal resident gastrointestinal microflora contains many diverse populations of bacteria which play an essential role in the development and well being of the host [67]. By continual release of antibiotic proteins, specialize ...
Student Handout 1 Key - 3D Molecular Designs
... In particular, the plasma membrane of mammalian red blood cells (erythrocytes) has been the focus of cell membrane study because these cells do not contain nuclei or internal membranes. They represent a source from which a pure plasma membrane may be easily isolated for analysis. In 1925, Dutch scie ...
... In particular, the plasma membrane of mammalian red blood cells (erythrocytes) has been the focus of cell membrane study because these cells do not contain nuclei or internal membranes. They represent a source from which a pure plasma membrane may be easily isolated for analysis. In 1925, Dutch scie ...
Physical properties of lipid bilayer membranes: relevance to
... the purple membrane showed PM about 6 to 10 times smaller than that of a fluid-phase lipid bilayer [37]. The interior of integral membrane proteins is practically not permeable to oxygen [41]. Based on these permeability coefficients the possible oxygen concentration differences across the cell plas ...
... the purple membrane showed PM about 6 to 10 times smaller than that of a fluid-phase lipid bilayer [37]. The interior of integral membrane proteins is practically not permeable to oxygen [41]. Based on these permeability coefficients the possible oxygen concentration differences across the cell plas ...
Pseudomonas spp.-induced systemic resistance
... cultures in response to rhizobacterial elicitors that could be linked to the development of ISR in whole plants (Van Loon et al., 2008). This oxidative burst was similar to that induced by oligosaccharides in grapevine suspension cells (Aziz et al., 2004, 2007), in which this response was found to b ...
... cultures in response to rhizobacterial elicitors that could be linked to the development of ISR in whole plants (Van Loon et al., 2008). This oxidative burst was similar to that induced by oligosaccharides in grapevine suspension cells (Aziz et al., 2004, 2007), in which this response was found to b ...
Microbiology with basis immunology_1
... A. Examination of morphological properties B. Examination of reducing properties C. Examination of biochemical properties D. Examination of tinctorial properties E. Examination of antigenic properties ANSWER: E Humoral factor of a nonspecific resistance to infection is: A. NK cells B. unbroken skin ...
... A. Examination of morphological properties B. Examination of reducing properties C. Examination of biochemical properties D. Examination of tinctorial properties E. Examination of antigenic properties ANSWER: E Humoral factor of a nonspecific resistance to infection is: A. NK cells B. unbroken skin ...
Spatiotemporal distribution of different extracellular polymeric
... compare samples from wild-type and/or mutant cells, cultivated in different conditions, against each other. The observed differences are usually attributed to the altered environmental condition. However, sometimes only the biofilm evolution rate is altered, and not its intrinsic features or evoluti ...
... compare samples from wild-type and/or mutant cells, cultivated in different conditions, against each other. The observed differences are usually attributed to the altered environmental condition. However, sometimes only the biofilm evolution rate is altered, and not its intrinsic features or evoluti ...
Commentary
... of Salp15 to OspC enhances B. burgdorferi infection or whether Salp15 does this alone. Mouse colonization experiments were conducted with a mixture of bacteria and soluble Salp15. A more convincing argument for the in vivo significance of Salp15–B. burgdorferi binding could be made if the bacteria w ...
... of Salp15 to OspC enhances B. burgdorferi infection or whether Salp15 does this alone. Mouse colonization experiments were conducted with a mixture of bacteria and soluble Salp15. A more convincing argument for the in vivo significance of Salp15–B. burgdorferi binding could be made if the bacteria w ...
(FT-IR) spectroscopy: A rapid tool for detection and analysis of
... Gram-positive bacteria have a thicker and rigid layer of peptidoglycan (PG, 40-80% by weight of the cell wall) than Gram-negative bacteria (10% by weight of the cell wall). The primary structure consists of parallel polysaccharide chains of alternating N-acetylglucosamine (NAG) and N-acetylmuramic a ...
... Gram-positive bacteria have a thicker and rigid layer of peptidoglycan (PG, 40-80% by weight of the cell wall) than Gram-negative bacteria (10% by weight of the cell wall). The primary structure consists of parallel polysaccharide chains of alternating N-acetylglucosamine (NAG) and N-acetylmuramic a ...
Presoaking reticular polypropylene meshes in antiseptic solution
... were observed for the mixture allicin-CHX (p<0.05). CHX was more effective than vancomycin (p<0.05), and q Fig 3. Bacterial colonization of the meshes allicin allowed bacterial colonization of the inhibition after 72 h of the contamination (x2,000). zone 24 h after Sa contamination [Figs. 1, 2]. 3.1 ...
... were observed for the mixture allicin-CHX (p<0.05). CHX was more effective than vancomycin (p<0.05), and q Fig 3. Bacterial colonization of the meshes allicin allowed bacterial colonization of the inhibition after 72 h of the contamination (x2,000). zone 24 h after Sa contamination [Figs. 1, 2]. 3.1 ...
Example of a scientific poster - SPUR
... although the data suggested some difference in proportion. Based on these results, we hypothesize that the increase in cell proliferation in CagA Drosophila is dependent upon the microbiota and is incurred because of an altered intestinal bacterial community. To test this hypothesis, we measured rat ...
... although the data suggested some difference in proportion. Based on these results, we hypothesize that the increase in cell proliferation in CagA Drosophila is dependent upon the microbiota and is incurred because of an altered intestinal bacterial community. To test this hypothesis, we measured rat ...
Gram`s staining - Micro-Rao
... Certain group of bacteria can display variable response to the stain, which can be due to growth stress (e.g., unsuitable nutrients, temperatures, pHs, or electrolytes) that results in a number of nonviable, gram-negative cells in a gram positive culture, but certain bacterial species are known for ...
... Certain group of bacteria can display variable response to the stain, which can be due to growth stress (e.g., unsuitable nutrients, temperatures, pHs, or electrolytes) that results in a number of nonviable, gram-negative cells in a gram positive culture, but certain bacterial species are known for ...
Leveau2008 - Johan Leveau
... tissue can be subdivided into three main categories (Fig. 1), similar to those used to classify bacterial interactions with plants or animals: extracellular necrotrophy, extracellular biotrophy, and endocellular biotrophy. Each of these interactions is characterized by a set of mycophagy determinant ...
... tissue can be subdivided into three main categories (Fig. 1), similar to those used to classify bacterial interactions with plants or animals: extracellular necrotrophy, extracellular biotrophy, and endocellular biotrophy. Each of these interactions is characterized by a set of mycophagy determinant ...
MS Prokaryotes
... and recycle nutrients back into the environment. This recycling of nutrients, such as nitrogen, is essential for living organisms. Organisms cannot produce nutrients, so they must come from other sources. We get nutrients from the food we eat; plants get them from the soil. How do these nutrients ge ...
... and recycle nutrients back into the environment. This recycling of nutrients, such as nitrogen, is essential for living organisms. Organisms cannot produce nutrients, so they must come from other sources. We get nutrients from the food we eat; plants get them from the soil. How do these nutrients ge ...
Journal of Bacteriology 186:
... Transparent variants persist in the nasopharynx in vivo and show greater adherence to human lung epithelial cells. However, experiments performed with an adult mouse model of sepsis showed a strong selection for organisms with the opaque morphology during invasive infections (28). Genetic experiment ...
... Transparent variants persist in the nasopharynx in vivo and show greater adherence to human lung epithelial cells. However, experiments performed with an adult mouse model of sepsis showed a strong selection for organisms with the opaque morphology during invasive infections (28). Genetic experiment ...
Journal of Bacteriology
... The gram-negative soil bacterium Rhizobium species attaches to the root hair tips of leguminous plants as a first step in the infection process leading to a nitrogen-fixing symbiosis. In fast-growing rhizobia the nodulation genes are located on a large plasmid, the so-called Sym plasmid. The molecul ...
... The gram-negative soil bacterium Rhizobium species attaches to the root hair tips of leguminous plants as a first step in the infection process leading to a nitrogen-fixing symbiosis. In fast-growing rhizobia the nodulation genes are located on a large plasmid, the so-called Sym plasmid. The molecul ...
First description of giant Archaea (Thaumarchaeota)
... This bacteria-free phenotype occurs much less frequently than the bacteria associated one (F. Muller and O. Gros, personal observations). The cell walls of both archaeal cell genotypes are considerably different from bacterial cell walls described for Gram-negative or Gram-positive marine bacteria ( ...
... This bacteria-free phenotype occurs much less frequently than the bacteria associated one (F. Muller and O. Gros, personal observations). The cell walls of both archaeal cell genotypes are considerably different from bacterial cell walls described for Gram-negative or Gram-positive marine bacteria ( ...
DENTAL PLAQUE
... microorganisms to adhere to one another, a process known as coaggregation. This process occurs primarily through the highly specific stereochemical interaction of protein and carbohydrate molecules located on the bacterial cell surfaces, in addition to the less specific interactions resulting from h ...
... microorganisms to adhere to one another, a process known as coaggregation. This process occurs primarily through the highly specific stereochemical interaction of protein and carbohydrate molecules located on the bacterial cell surfaces, in addition to the less specific interactions resulting from h ...
Expression of and Cytokine Activation by Eschevichia coli Curi
... Curli are a novel class of bacterial surface structures, expressed in both Escherichia coli and Salmonella, that are characterized by their ability to bind serum protein fibronectin [10, 11]. Colonies of curli-expressing strains typically stain positive for Congo red, allowing for an initial rapid s ...
... Curli are a novel class of bacterial surface structures, expressed in both Escherichia coli and Salmonella, that are characterized by their ability to bind serum protein fibronectin [10, 11]. Colonies of curli-expressing strains typically stain positive for Congo red, allowing for an initial rapid s ...
Bacteria viability assessment after photocatalytic treatment Yanling Cai Maria Strømme
... bacterial viability after photocatalytic treatment. When assessing bacterial viability and, in particular, bacteria in biofilm form, it is often necessary to use several methods in concert to get reliable results. Currently, methods widely used in bacterial viability analysis include indirect method ...
... bacterial viability after photocatalytic treatment. When assessing bacterial viability and, in particular, bacteria in biofilm form, it is often necessary to use several methods in concert to get reliable results. Currently, methods widely used in bacterial viability analysis include indirect method ...
Title Nanofiltration and reverse osmosis surface topographical
... filtration processes. The consequence of these pronounced surface defects on membranes should not be neglected since it is unclear how these might contribute to the rate of bacterial adhesion and potentially the characteristics of the subsequent biofilm. This work provides a framework by which novel ...
... filtration processes. The consequence of these pronounced surface defects on membranes should not be neglected since it is unclear how these might contribute to the rate of bacterial adhesion and potentially the characteristics of the subsequent biofilm. This work provides a framework by which novel ...
AN ELECTRON-MICROSCOPE STUDY OF NATURALLY
... 1. One drop was placed on a plate of GC medium and spread evenly with a loop while the plate was rotated horizontally on an electrically operated turntable. 2. One drop was placed on the surface of a freshly split piece of mica and spread evenly over its surface with the edge of another mica strip. ...
... 1. One drop was placed on a plate of GC medium and spread evenly with a loop while the plate was rotated horizontally on an electrically operated turntable. 2. One drop was placed on the surface of a freshly split piece of mica and spread evenly over its surface with the edge of another mica strip. ...
Ranjit Kumar
... Bacterial luminescence has been studied most extensively in several marine bacteria. Bacterial luminescence is due to the action of the enzyme called luciferase. The luminescent bacteria exist in nature either as free living bacteria or in symbiotic association ship with certain marine organisms. Re ...
... Bacterial luminescence has been studied most extensively in several marine bacteria. Bacterial luminescence is due to the action of the enzyme called luciferase. The luminescent bacteria exist in nature either as free living bacteria or in symbiotic association ship with certain marine organisms. Re ...
Structural Medicine II - European Science Foundation
... the systematic study of the unique chemical fingerprints linked to all cellular processes. Lipids have long been neglected. Why? Because tools to analyse lipid diversity have not been available until recently. Through the development of mass spectrometric methods we can now for the first time quanti ...
... the systematic study of the unique chemical fingerprints linked to all cellular processes. Lipids have long been neglected. Why? Because tools to analyse lipid diversity have not been available until recently. Through the development of mass spectrometric methods we can now for the first time quanti ...
Root Hairs. Plant Cell Monographs.
... that result in illness or pollution. For example, nitrogen-fixing root nodules will not develop without rhizobia attaching to and colonizing the root surface. (For more on nodule development, see Limpens and Bisseling 2008). Although a completely attachment-minus (Att−) rhizobial mutant has not been ...
... that result in illness or pollution. For example, nitrogen-fixing root nodules will not develop without rhizobia attaching to and colonizing the root surface. (For more on nodule development, see Limpens and Bisseling 2008). Although a completely attachment-minus (Att−) rhizobial mutant has not been ...
Lipopolysaccharide
Lipopolysaccharides (LPS), also known as lipoglycans and endotoxin, are large molecules consisting of a lipid and a polysaccharide composed of O-antigen, outer core and inner core joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, and elicit strong immune responses in animals.The term lipooligosaccharide (""LOS"") is used to refer to a low molecular weight form of bacterial lipopolysaccharides.